Study on Immobilization Method of Two-species Mycelial Pellet for Degradation of Lignocellulose
In our research，enzyme production ability of immobilization mycelial pellet was used as an index， the immobilization mycelial pellet was immobilized with lignin-degrading fungus G-13 and cellulose-degrading bacteria X10-1-2. The influence of five factors ( addition time，inoculation amount of cellulo...
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Harbin University of Science and Technology Publications
2018-08-01
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| Series: | Journal of Harbin University of Science and Technology |
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| Online Access: | https://hlgxb.hrbust.edu.cn/#/digest?ArticleID=1570 |
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| author | GUO Wei YAN Hong PEI Lei |
| author_facet | GUO Wei YAN Hong PEI Lei |
| author_sort | GUO Wei |
| collection | DOAJ |
| description | In our research，enzyme production ability of immobilization mycelial pellet was used as an index， the immobilization mycelial pellet was immobilized with lignin-degrading fungus G-13 and cellulose-degrading bacteria X10-1-2. The influence of five factors ( addition time，inoculation amount of cellulose-degrading bacteria， pH，temperature and rotational speed) to Mycelium pellet，ligninase production and cellulase production were studied. The results indicated that X10-1-2 and G-13 were added simultaneously and the inoculation amount was 20% ，pH was 5，culture temperature was 28℃，rotational speed was 160 r/min，the manganese( MnP) ; laccase ( Lac) ; lignin peroxidase( Lip) ; cellulose; hemicellulose were 31. 246 U/L; 16. 048 U/L; 157. 528 U/L; 197. 063 U/L; 215. 81 U/L，respectively. X10-1-2 and G-13 have the complementary advantage on the immobilization process，develope the synergy effects and promote the production of ligninase. The immobilization mycelial pellet was white，smooth surface，uniform size and good elasticity，which can satisfy practical needs |
| format | Article |
| id | doaj-art-eee56a73e0c24de3b9c56ea2082aab83 |
| institution | Kabale University |
| issn | 1007-2683 |
| language | zho |
| publishDate | 2018-08-01 |
| publisher | Harbin University of Science and Technology Publications |
| record_format | Article |
| series | Journal of Harbin University of Science and Technology |
| spelling | doaj-art-eee56a73e0c24de3b9c56ea2082aab832025-08-22T09:32:25ZzhoHarbin University of Science and Technology PublicationsJournal of Harbin University of Science and Technology1007-26832018-08-01230412713210.15938/j.jhust.2018.04.024Study on Immobilization Method of Two-species Mycelial Pellet for Degradation of LignocelluloseGUO Wei0YAN Hong1PEI Lei2School of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China;Key Laboratory of Green Chemical Technology of College of Heilongjiang Province, Harbin 150040, ChinaSchool of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China;Key Laboratory of Green Chemical Technology of College of Heilongjiang Province, Harbin 150040, ChinaSchool of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China;Key Laboratory of Green Chemical Technology of College of Heilongjiang Province, Harbin 150040, ChinaIn our research，enzyme production ability of immobilization mycelial pellet was used as an index， the immobilization mycelial pellet was immobilized with lignin-degrading fungus G-13 and cellulose-degrading bacteria X10-1-2. The influence of five factors ( addition time，inoculation amount of cellulose-degrading bacteria， pH，temperature and rotational speed) to Mycelium pellet，ligninase production and cellulase production were studied. The results indicated that X10-1-2 and G-13 were added simultaneously and the inoculation amount was 20% ，pH was 5，culture temperature was 28℃，rotational speed was 160 r/min，the manganese( MnP) ; laccase ( Lac) ; lignin peroxidase( Lip) ; cellulose; hemicellulose were 31. 246 U/L; 16. 048 U/L; 157. 528 U/L; 197. 063 U/L; 215. 81 U/L，respectively. X10-1-2 and G-13 have the complementary advantage on the immobilization process，develope the synergy effects and promote the production of ligninase. The immobilization mycelial pellet was white，smooth surface，uniform size and good elasticity，which can satisfy practical needshttps://hlgxb.hrbust.edu.cn/#/digest?ArticleID=1570mycelia pelletslignin-degrading funguscellulose-degrading bacterialigninasecellulase |
| spellingShingle | GUO Wei YAN Hong PEI Lei Study on Immobilization Method of Two-species Mycelial Pellet for Degradation of Lignocellulose Journal of Harbin University of Science and Technology mycelia pellets lignin-degrading fungus cellulose-degrading bacteria ligninase cellulase |
| title | Study on Immobilization Method of Two-species Mycelial Pellet for Degradation of Lignocellulose |
| title_full | Study on Immobilization Method of Two-species Mycelial Pellet for Degradation of Lignocellulose |
| title_fullStr | Study on Immobilization Method of Two-species Mycelial Pellet for Degradation of Lignocellulose |
| title_full_unstemmed | Study on Immobilization Method of Two-species Mycelial Pellet for Degradation of Lignocellulose |
| title_short | Study on Immobilization Method of Two-species Mycelial Pellet for Degradation of Lignocellulose |
| title_sort | study on immobilization method of two species mycelial pellet for degradation of lignocellulose |
| topic | mycelia pellets lignin-degrading fungus cellulose-degrading bacteria ligninase cellulase |
| url | https://hlgxb.hrbust.edu.cn/#/digest?ArticleID=1570 |
| work_keys_str_mv | AT guowei studyonimmobilizationmethodoftwospeciesmycelialpelletfordegradationoflignocellulose AT yanhong studyonimmobilizationmethodoftwospeciesmycelialpelletfordegradationoflignocellulose AT peilei studyonimmobilizationmethodoftwospeciesmycelialpelletfordegradationoflignocellulose |