Mitochondria-Targeting and Oxygen Self-Supplying Eccentric Hollow Nanoplatform for Enhanced Breast Cancer Photodynamic Therapy
Photodynamic therapy (PDT) has received increasing attention for tumor therapy due to its minimal invasiveness and spatiotemporal selectivity. However, the poor targeting of photosensitizer and hypoxia of the tumor microenvironment limit the PDT efficacy. Herein, eccentric hollow mesoporous organic...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2024/6618388 |
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| author | Jing Li Yu Wang Jun Tao Xiaodan Su Feipeng Zhu Wei Lu Xiaolin Han Meng Dang Lixing Weng |
| author_facet | Jing Li Yu Wang Jun Tao Xiaodan Su Feipeng Zhu Wei Lu Xiaolin Han Meng Dang Lixing Weng |
| author_sort | Jing Li |
| collection | DOAJ |
| description | Photodynamic therapy (PDT) has received increasing attention for tumor therapy due to its minimal invasiveness and spatiotemporal selectivity. However, the poor targeting of photosensitizer and hypoxia of the tumor microenvironment limit the PDT efficacy. Herein, eccentric hollow mesoporous organic silica nanoparticles (EHMONs) are prepared by anisotropic encapsulation and hydrothermal etching for constructing PDT nanoplatforms with targeting and hypoxia-alleviating properties. The prepared EHMONs possess a unique eccentric hollow structure, a uniform size (300 nm), a large cavity, and ordered mesoporous channels (2.3 nm). The EHMONs are modified with the mitochondria-targeting molecule triphenylphosphine (CTPP) and photosensitizers chlorin e6 (Ce6). Oxygen-carrying compound perfluorocarbons (PFCs) are further loaded in the internal cavity of EHMONs. Hemolytic assays and in vitro toxicity experiments show that the EHMONs-Ce6-CTPP possesses very good biocompatibility and can target mitochondria of triple-negative breast cancer, thus increasing the accumulation of photosensitizers Ce6 at mitochondria after entering cancer cells. The EHMONs-Ce6-CTPP@PFCs with oxygen-carrying ability can alleviate hypoxia after entering in the cancer cell. Phantom and cellular experiments show that the EHMONs-Ce6-CTPP@PFCs produce more singlet oxygen reactive oxygen species (ROSs). Thus, in vitro and in vivo experiments demonstrated that the EHMONs-Ce6-CTPP@PFCs showed excellent treatment effects for triple-negative breast cancer. This research provides a new method for a targeting and oxygen-carrying nanoplatform for enhancing PDF effectiveness. |
| format | Article |
| id | doaj-art-eedf0a5e0eab41c38ab4db48c9a5f013 |
| institution | Kabale University |
| issn | 1687-479X |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-eedf0a5e0eab41c38ab4db48c9a5f0132025-02-03T01:29:31ZengWileyBioinorganic Chemistry and Applications1687-479X2024-01-01202410.1155/2024/6618388Mitochondria-Targeting and Oxygen Self-Supplying Eccentric Hollow Nanoplatform for Enhanced Breast Cancer Photodynamic TherapyJing Li0Yu Wang1Jun Tao2Xiaodan Su3Feipeng Zhu4Wei Lu5Xiaolin Han6Meng Dang7Lixing Weng8Key Laboratory for Organic Electronics & Information Displays and Jiangsu Key Laboratory for BiosensorsKey Laboratory for Organic Electronics & Information Displays and Jiangsu Key Laboratory for BiosensorsKey Laboratory for Organic Electronics & Information Displays and Jiangsu Key Laboratory for BiosensorsKey Laboratory for Organic Electronics & Information Displays and Jiangsu Key Laboratory for BiosensorsDepartment of RadiologyKey Laboratory for Organic Electronics & Information Displays and Jiangsu Key Laboratory for BiosensorsKey Laboratory for Organic Electronics & Information Displays and Jiangsu Key Laboratory for BiosensorsKey Laboratory for Organic Electronics & Information Displays and Jiangsu Key Laboratory for BiosensorsCollege of Geography and Biological InformationPhotodynamic therapy (PDT) has received increasing attention for tumor therapy due to its minimal invasiveness and spatiotemporal selectivity. However, the poor targeting of photosensitizer and hypoxia of the tumor microenvironment limit the PDT efficacy. Herein, eccentric hollow mesoporous organic silica nanoparticles (EHMONs) are prepared by anisotropic encapsulation and hydrothermal etching for constructing PDT nanoplatforms with targeting and hypoxia-alleviating properties. The prepared EHMONs possess a unique eccentric hollow structure, a uniform size (300 nm), a large cavity, and ordered mesoporous channels (2.3 nm). The EHMONs are modified with the mitochondria-targeting molecule triphenylphosphine (CTPP) and photosensitizers chlorin e6 (Ce6). Oxygen-carrying compound perfluorocarbons (PFCs) are further loaded in the internal cavity of EHMONs. Hemolytic assays and in vitro toxicity experiments show that the EHMONs-Ce6-CTPP possesses very good biocompatibility and can target mitochondria of triple-negative breast cancer, thus increasing the accumulation of photosensitizers Ce6 at mitochondria after entering cancer cells. The EHMONs-Ce6-CTPP@PFCs with oxygen-carrying ability can alleviate hypoxia after entering in the cancer cell. Phantom and cellular experiments show that the EHMONs-Ce6-CTPP@PFCs produce more singlet oxygen reactive oxygen species (ROSs). Thus, in vitro and in vivo experiments demonstrated that the EHMONs-Ce6-CTPP@PFCs showed excellent treatment effects for triple-negative breast cancer. This research provides a new method for a targeting and oxygen-carrying nanoplatform for enhancing PDF effectiveness.http://dx.doi.org/10.1155/2024/6618388 |
| spellingShingle | Jing Li Yu Wang Jun Tao Xiaodan Su Feipeng Zhu Wei Lu Xiaolin Han Meng Dang Lixing Weng Mitochondria-Targeting and Oxygen Self-Supplying Eccentric Hollow Nanoplatform for Enhanced Breast Cancer Photodynamic Therapy Bioinorganic Chemistry and Applications |
| title | Mitochondria-Targeting and Oxygen Self-Supplying Eccentric Hollow Nanoplatform for Enhanced Breast Cancer Photodynamic Therapy |
| title_full | Mitochondria-Targeting and Oxygen Self-Supplying Eccentric Hollow Nanoplatform for Enhanced Breast Cancer Photodynamic Therapy |
| title_fullStr | Mitochondria-Targeting and Oxygen Self-Supplying Eccentric Hollow Nanoplatform for Enhanced Breast Cancer Photodynamic Therapy |
| title_full_unstemmed | Mitochondria-Targeting and Oxygen Self-Supplying Eccentric Hollow Nanoplatform for Enhanced Breast Cancer Photodynamic Therapy |
| title_short | Mitochondria-Targeting and Oxygen Self-Supplying Eccentric Hollow Nanoplatform for Enhanced Breast Cancer Photodynamic Therapy |
| title_sort | mitochondria targeting and oxygen self supplying eccentric hollow nanoplatform for enhanced breast cancer photodynamic therapy |
| url | http://dx.doi.org/10.1155/2024/6618388 |
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