Formulation and evaluation of texture nutritional and sensory properties of plant-based cheese alternatives

This research studied the production processes and the physical, chemical, microbial, and sensory properties of appropriate formulas for plant-based cheese analogs products. The study investigated three factors: modified starch, categorized into three levels (8.00 %, 10.00 %, and 12.00 %); soy milk,...

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Main Authors: Orawan Oupathumpanont, Suchanart Thippayajan, Natthakan Pannarat, Piangthan Chaisingkan, Sopida Wisansakkul
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Applied Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772502225001878
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author Orawan Oupathumpanont
Suchanart Thippayajan
Natthakan Pannarat
Piangthan Chaisingkan
Sopida Wisansakkul
author_facet Orawan Oupathumpanont
Suchanart Thippayajan
Natthakan Pannarat
Piangthan Chaisingkan
Sopida Wisansakkul
author_sort Orawan Oupathumpanont
collection DOAJ
description This research studied the production processes and the physical, chemical, microbial, and sensory properties of appropriate formulas for plant-based cheese analogs products. The study investigated three factors: modified starch, categorized into three levels (8.00 %, 10.00 %, and 12.00 %); soy milk, classified into three levels (46.00 %, 49.00 %, and 52.00 %); and buttermilk flavor, differentiated into two levels (0.75 % and 1.00 %). A factorial design experiment resulted in 18 production formulas. The appropriate formula was selected based on chemical properties such as moisture, physical properties such as color, texture, meltability, and flowability with surface structural characteristics determined by scanning electron microscopy (SEM). The values obtained were grouped using hierarchical cluster analysis and the preferences of 60 consumers were tested using a 9-point hedonic scale. The plant-based cheese analogs were analyzed for nutritional properties, microorganisms, and consumer acceptance. The suitable formula for producing plant-base cheese analogs is formula number 4 with the following ingredients: casein protein powder, protein sludge from soy milk, soy milk, coconut oil, modified starch, carrageenan, xanthan gum, and buttermilk flavoring agent with percentages of 15.00, 15.00, 49.00, 11.00, 8.00, 0.70, 0.30. and 1.00, respectively. The nutritional value of 100 g. of plant-based cheese analogs product consisted of total energy 335.88 kilocalories, protein 11.42 g., fat 22.88 g., carbohydrates 21.07 g., dietary fiber 8.33 g., vitamin B2 0.193 mg., iron 0.93 mg., and calcium 104.82 mg. According to the Ministry of Public Health Announcement No 416 BE. 2021, microbiological quality is within the standard criteria for food pathogenic microorganisms. The overall liking score was moderate, suggesting that this product met the needs of lacto-vegetarian and plant-based consumers. This research results in plant-based products with taste, texture, and nutritional adequacy that are acceptable to consumers, and increase the choice of general consumers and lacto-vegetarian groups.
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publishDate 2025-06-01
publisher Elsevier
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series Applied Food Research
spelling doaj-art-eeddd10b0e2e46ce988a0f3e5cd295c52025-08-20T03:45:18ZengElsevierApplied Food Research2772-50222025-06-015110087710.1016/j.afres.2025.100877Formulation and evaluation of texture nutritional and sensory properties of plant-based cheese alternativesOrawan Oupathumpanont0Suchanart Thippayajan1Natthakan Pannarat2Piangthan Chaisingkan3Sopida Wisansakkul4Department of Food and Nutrition, Faculty of Home Economics Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand; Corresponding authors.Department of Home Economics Technology, Faculty of Home Economics Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, ThailandDepartment of Home Economics Technology, Faculty of Home Economics Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, ThailandDepartment of Home Economics Technology, Faculty of Home Economics Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, ThailandDepartment of Home Economics, Faculty of Home Economics Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand; Corresponding authors.This research studied the production processes and the physical, chemical, microbial, and sensory properties of appropriate formulas for plant-based cheese analogs products. The study investigated three factors: modified starch, categorized into three levels (8.00 %, 10.00 %, and 12.00 %); soy milk, classified into three levels (46.00 %, 49.00 %, and 52.00 %); and buttermilk flavor, differentiated into two levels (0.75 % and 1.00 %). A factorial design experiment resulted in 18 production formulas. The appropriate formula was selected based on chemical properties such as moisture, physical properties such as color, texture, meltability, and flowability with surface structural characteristics determined by scanning electron microscopy (SEM). The values obtained were grouped using hierarchical cluster analysis and the preferences of 60 consumers were tested using a 9-point hedonic scale. The plant-based cheese analogs were analyzed for nutritional properties, microorganisms, and consumer acceptance. The suitable formula for producing plant-base cheese analogs is formula number 4 with the following ingredients: casein protein powder, protein sludge from soy milk, soy milk, coconut oil, modified starch, carrageenan, xanthan gum, and buttermilk flavoring agent with percentages of 15.00, 15.00, 49.00, 11.00, 8.00, 0.70, 0.30. and 1.00, respectively. The nutritional value of 100 g. of plant-based cheese analogs product consisted of total energy 335.88 kilocalories, protein 11.42 g., fat 22.88 g., carbohydrates 21.07 g., dietary fiber 8.33 g., vitamin B2 0.193 mg., iron 0.93 mg., and calcium 104.82 mg. According to the Ministry of Public Health Announcement No 416 BE. 2021, microbiological quality is within the standard criteria for food pathogenic microorganisms. The overall liking score was moderate, suggesting that this product met the needs of lacto-vegetarian and plant-based consumers. This research results in plant-based products with taste, texture, and nutritional adequacy that are acceptable to consumers, and increase the choice of general consumers and lacto-vegetarian groups.http://www.sciencedirect.com/science/article/pii/S2772502225001878Soy milkPlant-basedConsumer acceptanceLacto-veganCheese
spellingShingle Orawan Oupathumpanont
Suchanart Thippayajan
Natthakan Pannarat
Piangthan Chaisingkan
Sopida Wisansakkul
Formulation and evaluation of texture nutritional and sensory properties of plant-based cheese alternatives
Applied Food Research
Soy milk
Plant-based
Consumer acceptance
Lacto-vegan
Cheese
title Formulation and evaluation of texture nutritional and sensory properties of plant-based cheese alternatives
title_full Formulation and evaluation of texture nutritional and sensory properties of plant-based cheese alternatives
title_fullStr Formulation and evaluation of texture nutritional and sensory properties of plant-based cheese alternatives
title_full_unstemmed Formulation and evaluation of texture nutritional and sensory properties of plant-based cheese alternatives
title_short Formulation and evaluation of texture nutritional and sensory properties of plant-based cheese alternatives
title_sort formulation and evaluation of texture nutritional and sensory properties of plant based cheese alternatives
topic Soy milk
Plant-based
Consumer acceptance
Lacto-vegan
Cheese
url http://www.sciencedirect.com/science/article/pii/S2772502225001878
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AT natthakanpannarat formulationandevaluationoftexturenutritionalandsensorypropertiesofplantbasedcheesealternatives
AT piangthanchaisingkan formulationandevaluationoftexturenutritionalandsensorypropertiesofplantbasedcheesealternatives
AT sopidawisansakkul formulationandevaluationoftexturenutritionalandsensorypropertiesofplantbasedcheesealternatives