Hydroxylated fullerene promotes fracture healing in ovariectomized combined type 2 diabetic mice
Abstract Background The aim of this investigation was to assess the interventional role of hydroxylated fullerene in this model by histological, imaging, and biomechanical means in order to inform the treatment of fracture healing in a population of menopausal women with type 2 diabetes mellitus (T2...
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| Format: | Article |
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BMC
2025-06-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Online Access: | https://doi.org/10.1186/s13018-025-05997-5 |
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| author | Yunpeng Hu Linjie Feng Jie Li Fuyuan Cao Xiaoli Hou Wei Chen Yimeng Zhang Lei Xing Jingyuan Gao Liu Zhang Ye Liu Faming Tian |
| author_facet | Yunpeng Hu Linjie Feng Jie Li Fuyuan Cao Xiaoli Hou Wei Chen Yimeng Zhang Lei Xing Jingyuan Gao Liu Zhang Ye Liu Faming Tian |
| author_sort | Yunpeng Hu |
| collection | DOAJ |
| description | Abstract Background The aim of this investigation was to assess the interventional role of hydroxylated fullerene in this model by histological, imaging, and biomechanical means in order to inform the treatment of fracture healing in a population of menopausal women with type 2 diabetes mellitus (T2DM). Methods Sixty-four female 8-week-old C57BL/6 mice were randomly divided into four groups: fracture (F), OVX + fracture (OF), T2DM + OVX + fracture (DOF), and hydroxylated fullerene-treated DOF (DOFF). A closed fracture was established in the right tibia of each mouse, which was performed 8 weeks after undergoing ovariectomy (OVX) in the OF, DOF, and DOFF groups. Hydroxylated fullerene (5 mg/kg, every two days) was administered intraperitoneally to the DOFF group starting the day after fracture. The right tibias were collected at 7 and 28 days post-fracture. Results The systemic administration of hydroxylated fullerene significantly increased the microstructural parameters of the callus (including bone volume fraction [BV/TV] and connectivity density [Conn.D]), promoted BMP-2 expression and inhibited TNF-α expression in the callus at 7 days post-fracture, and increased the expression of type I collagen (COL1) and osteocalcin (OCN) in the callus at 28 days post-fracture. Conclusion Hydroxylated fullerene may improve fracture healing in this diabetic mouse model by reducing the inflammatory response and promoting osteogenesis. These results suggest that hydroxylated fullerene may act as a therapeutic agent for fracture healing. |
| format | Article |
| id | doaj-art-eedc540563ab4488ad2b1c16c63f1f82 |
| institution | OA Journals |
| issn | 1749-799X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj-art-eedc540563ab4488ad2b1c16c63f1f822025-08-20T02:37:33ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2025-06-0120111310.1186/s13018-025-05997-5Hydroxylated fullerene promotes fracture healing in ovariectomized combined type 2 diabetic miceYunpeng Hu0Linjie Feng1Jie Li2Fuyuan Cao3Xiaoli Hou4Wei Chen5Yimeng Zhang6Lei Xing7Jingyuan Gao8Liu Zhang9Ye Liu10Faming Tian11School of Public Health, North China University of Science and TechnologyDepartment of Orthopedic Surgery, The 982th Hospital of Joint Logistic Force of Chinese People’s Liberation ArmyBeijing National Research Center for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of SciencesSchool of Public Health, North China University of Science and TechnologySchool of Public Health, North China University of Science and TechnologyDepartment of Orthopedic Surgery, Hebei Medical UniversityMedical Department, The First Hospital of Hebei Medical UniversitySchool of Public Health, North China University of Science and TechnologyDepartment of Rheumatology and Immunology, Affiliated Hospital of North China, University of Science and TechnologySchool of Public Health, North China University of Science and TechnologyDepartment of Anaesthesiology, Beijing Obstetrics and Gynecology Hospital, Capital Medical UniversitySchool of Public Health, North China University of Science and TechnologyAbstract Background The aim of this investigation was to assess the interventional role of hydroxylated fullerene in this model by histological, imaging, and biomechanical means in order to inform the treatment of fracture healing in a population of menopausal women with type 2 diabetes mellitus (T2DM). Methods Sixty-four female 8-week-old C57BL/6 mice were randomly divided into four groups: fracture (F), OVX + fracture (OF), T2DM + OVX + fracture (DOF), and hydroxylated fullerene-treated DOF (DOFF). A closed fracture was established in the right tibia of each mouse, which was performed 8 weeks after undergoing ovariectomy (OVX) in the OF, DOF, and DOFF groups. Hydroxylated fullerene (5 mg/kg, every two days) was administered intraperitoneally to the DOFF group starting the day after fracture. The right tibias were collected at 7 and 28 days post-fracture. Results The systemic administration of hydroxylated fullerene significantly increased the microstructural parameters of the callus (including bone volume fraction [BV/TV] and connectivity density [Conn.D]), promoted BMP-2 expression and inhibited TNF-α expression in the callus at 7 days post-fracture, and increased the expression of type I collagen (COL1) and osteocalcin (OCN) in the callus at 28 days post-fracture. Conclusion Hydroxylated fullerene may improve fracture healing in this diabetic mouse model by reducing the inflammatory response and promoting osteogenesis. These results suggest that hydroxylated fullerene may act as a therapeutic agent for fracture healing.https://doi.org/10.1186/s13018-025-05997-5 |
| spellingShingle | Yunpeng Hu Linjie Feng Jie Li Fuyuan Cao Xiaoli Hou Wei Chen Yimeng Zhang Lei Xing Jingyuan Gao Liu Zhang Ye Liu Faming Tian Hydroxylated fullerene promotes fracture healing in ovariectomized combined type 2 diabetic mice Journal of Orthopaedic Surgery and Research |
| title | Hydroxylated fullerene promotes fracture healing in ovariectomized combined type 2 diabetic mice |
| title_full | Hydroxylated fullerene promotes fracture healing in ovariectomized combined type 2 diabetic mice |
| title_fullStr | Hydroxylated fullerene promotes fracture healing in ovariectomized combined type 2 diabetic mice |
| title_full_unstemmed | Hydroxylated fullerene promotes fracture healing in ovariectomized combined type 2 diabetic mice |
| title_short | Hydroxylated fullerene promotes fracture healing in ovariectomized combined type 2 diabetic mice |
| title_sort | hydroxylated fullerene promotes fracture healing in ovariectomized combined type 2 diabetic mice |
| url | https://doi.org/10.1186/s13018-025-05997-5 |
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