Analysis of the CPZ/Wnt4 osteogenic pathway for high-bonding-strength composite-coated magnesium scaffolds through transcriptomics
Magnesium (Mg)-based scaffolds are garnering increasing attention as bone repair materials owing to their biodegradability and mechanical resemblance to natural bone. Their effectiveness can be augmented by incorporating surface coatings to meet clinical needs. However, the limited bonding strength...
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Elsevier
2024-10-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424002953 |
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| author | Zewen Shi Fang Yang Tianyu Du Qian Pang Chen Liu Yiwei Hu Weilai Zhu Xianjun Chen Zeming Chen Baiyang Song Xueqiang Yu Zhewei Ye Lin Shi Yabin Zhu Qingjiang Pang |
| author_facet | Zewen Shi Fang Yang Tianyu Du Qian Pang Chen Liu Yiwei Hu Weilai Zhu Xianjun Chen Zeming Chen Baiyang Song Xueqiang Yu Zhewei Ye Lin Shi Yabin Zhu Qingjiang Pang |
| author_sort | Zewen Shi |
| collection | DOAJ |
| description | Magnesium (Mg)-based scaffolds are garnering increasing attention as bone repair materials owing to their biodegradability and mechanical resemblance to natural bone. Their effectiveness can be augmented by incorporating surface coatings to meet clinical needs. However, the limited bonding strength and unclear mechanisms of these coatings have impeded the clinical utility of scaffolds. To address these issues, this study introduces a composite coating of high-bonding-strength polydopamine-microarc oxidation (PDA-MHA) on Mg-based scaffolds. The results showed that the PDA-MHA coating achieved a bonding strength of 40.56 ± 1.426 MPa with the Mg scaffold surface, effectively enhancing hydrophilicity and controlling degradation rates. Furthermore, the scaffold facilitated bone regeneration by influencing osteogenic markers such as RUNX-2, OPN, OCN, and VEGF. Transcriptomic analyses further demonstrated that the PDA-MHA/Mg scaffold upregulated carboxypeptidase Z expression and activated the Wnt-4/β-catenin signaling pathway, thereby promoting bone regeneration. Overall, this study demonstrated that PDA can synergistically enhance bone repair with Mg scaffold, broadening the application scenarios of Mg and PDA in the field of biomaterials. Moreover, this study provides a theoretical underpinning for the application and clinical translation of Mg-based scaffolds in bone tissue engineering endeavors. |
| format | Article |
| id | doaj-art-eedbc2ee174147f5acf445f9f2f85abe |
| institution | OA Journals |
| issn | 2590-0064 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-eedbc2ee174147f5acf445f9f2f85abe2025-08-20T01:54:45ZengElsevierMaterials Today Bio2590-00642024-10-012810123410.1016/j.mtbio.2024.101234Analysis of the CPZ/Wnt4 osteogenic pathway for high-bonding-strength composite-coated magnesium scaffolds through transcriptomicsZewen Shi0Fang Yang1Tianyu Du2Qian Pang3Chen Liu4Yiwei Hu5Weilai Zhu6Xianjun Chen7Zeming Chen8Baiyang Song9Xueqiang Yu10Zhewei Ye11Lin Shi12Yabin Zhu13Qingjiang Pang14Department of Orthopedics, Ningbo No. 2 Hospital, Ningbo, 315010, PR China; Health Science Center, Ningbo University, Ningbo, 315211, PR China; Department of Orthopaedics, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, PR ChinaHealth Science Center, Ningbo University, Ningbo, 315211, PR ChinaHealth Science Center, Ningbo University, Ningbo, 315211, PR ChinaHealth Science Center, Ningbo University, Ningbo, 315211, PR ChinaNingbo Branch of Chinese Academy of Ordnance Science, Ningbo, 315100, PR ChinaHealth Science Center, Ningbo University, Ningbo, 315211, PR ChinaHealth Science Center, Ningbo University, Ningbo, 315211, PR ChinaDepartment of Orthopedics, Ningbo No. 2 Hospital, Ningbo, 315010, PR ChinaDepartment of Orthopedics, Ningbo No. 2 Hospital, Ningbo, 315010, PR ChinaHealth Science Center, Ningbo University, Ningbo, 315211, PR ChinaDepartment of Orthopedics, Ningbo No. 2 Hospital, Ningbo, 315010, PR ChinaDepartment of Orthopaedics, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, PR ChinaDepartment of Orthopedics, Ningbo No. 2 Hospital, Ningbo, 315010, PR China; Corresponding author. Department of Orthopedics, Ningbo No. 2 Hospital, Ningbo, 315010, PR China.Health Science Center, Ningbo University, Ningbo, 315211, PR China; Corresponding author. Health Science Center, Ningbo University, Ningbo, 315211, PR China.Department of Orthopedics, Ningbo No. 2 Hospital, Ningbo, 315010, PR China; Health Science Center, Ningbo University, Ningbo, 315211, PR China; Corresponding author. Department of Orthopedics, Ningbo No. 2 Hospital, Ningbo, 315010, PR China.Magnesium (Mg)-based scaffolds are garnering increasing attention as bone repair materials owing to their biodegradability and mechanical resemblance to natural bone. Their effectiveness can be augmented by incorporating surface coatings to meet clinical needs. However, the limited bonding strength and unclear mechanisms of these coatings have impeded the clinical utility of scaffolds. To address these issues, this study introduces a composite coating of high-bonding-strength polydopamine-microarc oxidation (PDA-MHA) on Mg-based scaffolds. The results showed that the PDA-MHA coating achieved a bonding strength of 40.56 ± 1.426 MPa with the Mg scaffold surface, effectively enhancing hydrophilicity and controlling degradation rates. Furthermore, the scaffold facilitated bone regeneration by influencing osteogenic markers such as RUNX-2, OPN, OCN, and VEGF. Transcriptomic analyses further demonstrated that the PDA-MHA/Mg scaffold upregulated carboxypeptidase Z expression and activated the Wnt-4/β-catenin signaling pathway, thereby promoting bone regeneration. Overall, this study demonstrated that PDA can synergistically enhance bone repair with Mg scaffold, broadening the application scenarios of Mg and PDA in the field of biomaterials. Moreover, this study provides a theoretical underpinning for the application and clinical translation of Mg-based scaffolds in bone tissue engineering endeavors.http://www.sciencedirect.com/science/article/pii/S2590006424002953MagnesiumOsteogenesisPolydopamineHigh-bonding-strength coatingCarboxypeptidase Z |
| spellingShingle | Zewen Shi Fang Yang Tianyu Du Qian Pang Chen Liu Yiwei Hu Weilai Zhu Xianjun Chen Zeming Chen Baiyang Song Xueqiang Yu Zhewei Ye Lin Shi Yabin Zhu Qingjiang Pang Analysis of the CPZ/Wnt4 osteogenic pathway for high-bonding-strength composite-coated magnesium scaffolds through transcriptomics Materials Today Bio Magnesium Osteogenesis Polydopamine High-bonding-strength coating Carboxypeptidase Z |
| title | Analysis of the CPZ/Wnt4 osteogenic pathway for high-bonding-strength composite-coated magnesium scaffolds through transcriptomics |
| title_full | Analysis of the CPZ/Wnt4 osteogenic pathway for high-bonding-strength composite-coated magnesium scaffolds through transcriptomics |
| title_fullStr | Analysis of the CPZ/Wnt4 osteogenic pathway for high-bonding-strength composite-coated magnesium scaffolds through transcriptomics |
| title_full_unstemmed | Analysis of the CPZ/Wnt4 osteogenic pathway for high-bonding-strength composite-coated magnesium scaffolds through transcriptomics |
| title_short | Analysis of the CPZ/Wnt4 osteogenic pathway for high-bonding-strength composite-coated magnesium scaffolds through transcriptomics |
| title_sort | analysis of the cpz wnt4 osteogenic pathway for high bonding strength composite coated magnesium scaffolds through transcriptomics |
| topic | Magnesium Osteogenesis Polydopamine High-bonding-strength coating Carboxypeptidase Z |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424002953 |
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