The Modulation of PPARγ1 and PPARγ2 mRNA Expression by Ciglitazone in CD3/CD28-Activated Naïve and Memory CD4+ T Cells
Given their roles in immune regulation, the expression of the nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ) 1 and 2 isoforms was investigated in human naïve (CD45RA+) and memory (CD45RO+) CD4+ T cells. Stimulation of both types of cells via the CD3/CD28 pathway resulted in hi...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/849195 |
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| author | Mohd Nor Norazmi Rafeezul Mohamed Asma Abdullah Nurul Nik Soriani Yaacob |
| author_facet | Mohd Nor Norazmi Rafeezul Mohamed Asma Abdullah Nurul Nik Soriani Yaacob |
| author_sort | Mohd Nor Norazmi |
| collection | DOAJ |
| description | Given their roles in immune regulation, the expression of the nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ) 1 and 2 isoforms was investigated in human naïve (CD45RA+) and memory (CD45RO+) CD4+ T cells. Stimulation of both types of cells via the CD3/CD28 pathway resulted in high expression of both PPARγ receptors as measured by real-time PCR. Treatment with the PPARγ agonist, ciglitazone, increased PPARγ1 expression but decreased PPARγ2 expression in stimulated naïve and memory cells. Furthermore, when present, the magnitude of both PPARγ receptors expression was lower in naïve cells, perhaps suggesting a lower regulatory control of these cells. Similar profiles of selected proinflammatory cytokines were expressed by the two cell types following stimulation. The induction of PPARγ1 and suppression of PPARγ2 expressions in naïve and memory CD4+ T cells in the presence of ciglitazone suggest that the PPARγ subtypes may have different roles in the regulation of T-cell function. |
| format | Article |
| id | doaj-art-eed8630a94154d6ba2e0000bf1d45430 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-eed8630a94154d6ba2e0000bf1d454302025-02-03T06:44:49ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/849195849195The Modulation of PPARγ1 and PPARγ2 mRNA Expression by Ciglitazone in CD3/CD28-Activated Naïve and Memory CD4+ T CellsMohd Nor Norazmi0Rafeezul Mohamed1Asma Abdullah Nurul2Nik Soriani Yaacob3School of Health Sciences, Universiti Sains Malaysia, Kelantan, 16150 Kubang Kerian, MalaysiaSchool of Health Sciences, Universiti Sains Malaysia, Kelantan, 16150 Kubang Kerian, MalaysiaSchool of Dental Sciences, Universiti Sains Malaysia, Kelantan, 16150 Kubang Kerian, MalaysiaSchool of Medical Sciences, Universiti Sains Malaysia, Kelantan, 16150 Kubang Kerian, MalaysiaGiven their roles in immune regulation, the expression of the nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ) 1 and 2 isoforms was investigated in human naïve (CD45RA+) and memory (CD45RO+) CD4+ T cells. Stimulation of both types of cells via the CD3/CD28 pathway resulted in high expression of both PPARγ receptors as measured by real-time PCR. Treatment with the PPARγ agonist, ciglitazone, increased PPARγ1 expression but decreased PPARγ2 expression in stimulated naïve and memory cells. Furthermore, when present, the magnitude of both PPARγ receptors expression was lower in naïve cells, perhaps suggesting a lower regulatory control of these cells. Similar profiles of selected proinflammatory cytokines were expressed by the two cell types following stimulation. The induction of PPARγ1 and suppression of PPARγ2 expressions in naïve and memory CD4+ T cells in the presence of ciglitazone suggest that the PPARγ subtypes may have different roles in the regulation of T-cell function.http://dx.doi.org/10.1155/2012/849195 |
| spellingShingle | Mohd Nor Norazmi Rafeezul Mohamed Asma Abdullah Nurul Nik Soriani Yaacob The Modulation of PPARγ1 and PPARγ2 mRNA Expression by Ciglitazone in CD3/CD28-Activated Naïve and Memory CD4+ T Cells Clinical and Developmental Immunology |
| title | The Modulation of PPARγ1 and PPARγ2 mRNA Expression by Ciglitazone in CD3/CD28-Activated Naïve and Memory CD4+ T Cells |
| title_full | The Modulation of PPARγ1 and PPARγ2 mRNA Expression by Ciglitazone in CD3/CD28-Activated Naïve and Memory CD4+ T Cells |
| title_fullStr | The Modulation of PPARγ1 and PPARγ2 mRNA Expression by Ciglitazone in CD3/CD28-Activated Naïve and Memory CD4+ T Cells |
| title_full_unstemmed | The Modulation of PPARγ1 and PPARγ2 mRNA Expression by Ciglitazone in CD3/CD28-Activated Naïve and Memory CD4+ T Cells |
| title_short | The Modulation of PPARγ1 and PPARγ2 mRNA Expression by Ciglitazone in CD3/CD28-Activated Naïve and Memory CD4+ T Cells |
| title_sort | modulation of pparγ1 and pparγ2 mrna expression by ciglitazone in cd3 cd28 activated naive and memory cd4 t cells |
| url | http://dx.doi.org/10.1155/2012/849195 |
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