Genotoxicity Assessment of Multispecies Probiotics Using Reverse Mutation, Mammalian Chromosomal Aberration, and Rodent Micronucleus Tests
Genotoxicity assessment is carried out on freeze dried powder of cultured probiotics containing Lactobacillus rhamnosus LCR177, Bifidobacterium adolescentis BA286, and Pediococcus acidilactici PA318. Ames tests, in vitro mammalian chromosome aberration assay, and micronucleus tests in mouse peripher...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/254239 |
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| author | Yi-Jen Chiu Mun-Kit Nam Yueh-Ting Tsai Chun-Chi Huang Cheng-Chih Tsai |
| author_facet | Yi-Jen Chiu Mun-Kit Nam Yueh-Ting Tsai Chun-Chi Huang Cheng-Chih Tsai |
| author_sort | Yi-Jen Chiu |
| collection | DOAJ |
| description | Genotoxicity assessment is carried out on freeze dried powder of cultured probiotics containing Lactobacillus rhamnosus LCR177, Bifidobacterium adolescentis BA286, and Pediococcus acidilactici PA318. Ames tests, in vitro mammalian chromosome aberration assay, and micronucleus tests in mouse peripheral blood are performed. For 5 strains of Salmonella Typhimurium, the Ames tests show no increased reverse mutation upon exposure to the test substance. In CHO cells, the frequency of chromosome aberration does not increase in responding to the treatment of probiotics. Likewise, the frequency of micronucleated reticulocytes in probiotics-fed mice is indistinguishable from that in the negative control group. Taken together, the toxicity assessment studies suggest that the multispecies probiotic mixture does not have mutagenic effects on various organisms. |
| format | Article |
| id | doaj-art-eed18a8140ed448f8bfc68afbe14a055 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-eed18a8140ed448f8bfc68afbe14a0552025-02-03T01:31:13ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/254239254239Genotoxicity Assessment of Multispecies Probiotics Using Reverse Mutation, Mammalian Chromosomal Aberration, and Rodent Micronucleus TestsYi-Jen Chiu0Mun-Kit Nam1Yueh-Ting Tsai2Chun-Chi Huang3Cheng-Chih Tsai4Department of Food Science and Technology, Hungkuang University, No. 1018, Sector 6, Taiwan Boulevard, Shalu District, Taichung 43302, TaiwanSuper laboratory Ltd., New Taipei 24890, TaiwanSuper laboratory Ltd., New Taipei 24890, TaiwanDepartment of Food Science and Technology, Hungkuang University, No. 1018, Sector 6, Taiwan Boulevard, Shalu District, Taichung 43302, TaiwanDepartment of Food Science and Technology, Hungkuang University, No. 1018, Sector 6, Taiwan Boulevard, Shalu District, Taichung 43302, TaiwanGenotoxicity assessment is carried out on freeze dried powder of cultured probiotics containing Lactobacillus rhamnosus LCR177, Bifidobacterium adolescentis BA286, and Pediococcus acidilactici PA318. Ames tests, in vitro mammalian chromosome aberration assay, and micronucleus tests in mouse peripheral blood are performed. For 5 strains of Salmonella Typhimurium, the Ames tests show no increased reverse mutation upon exposure to the test substance. In CHO cells, the frequency of chromosome aberration does not increase in responding to the treatment of probiotics. Likewise, the frequency of micronucleated reticulocytes in probiotics-fed mice is indistinguishable from that in the negative control group. Taken together, the toxicity assessment studies suggest that the multispecies probiotic mixture does not have mutagenic effects on various organisms.http://dx.doi.org/10.1155/2013/254239 |
| spellingShingle | Yi-Jen Chiu Mun-Kit Nam Yueh-Ting Tsai Chun-Chi Huang Cheng-Chih Tsai Genotoxicity Assessment of Multispecies Probiotics Using Reverse Mutation, Mammalian Chromosomal Aberration, and Rodent Micronucleus Tests The Scientific World Journal |
| title | Genotoxicity Assessment of Multispecies Probiotics Using Reverse Mutation, Mammalian Chromosomal Aberration, and Rodent Micronucleus Tests |
| title_full | Genotoxicity Assessment of Multispecies Probiotics Using Reverse Mutation, Mammalian Chromosomal Aberration, and Rodent Micronucleus Tests |
| title_fullStr | Genotoxicity Assessment of Multispecies Probiotics Using Reverse Mutation, Mammalian Chromosomal Aberration, and Rodent Micronucleus Tests |
| title_full_unstemmed | Genotoxicity Assessment of Multispecies Probiotics Using Reverse Mutation, Mammalian Chromosomal Aberration, and Rodent Micronucleus Tests |
| title_short | Genotoxicity Assessment of Multispecies Probiotics Using Reverse Mutation, Mammalian Chromosomal Aberration, and Rodent Micronucleus Tests |
| title_sort | genotoxicity assessment of multispecies probiotics using reverse mutation mammalian chromosomal aberration and rodent micronucleus tests |
| url | http://dx.doi.org/10.1155/2013/254239 |
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