Hepatoma cell-derived exosomal SNORD52 mediates M2 macrophage polarization by activating the JAK2/STAT6 pathway
Abstract Background A recent study revealed the oncogenic role of box C/D small nucleolar RNA 52 (SNORD52) in hepatocellular carcinoma (HCC) by facilitating the aggressive phenotypes of hepatoma cells. However, the potential role of exosomal SNORD52 in macrophage polarization during HCC progression...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2025-01-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-024-01700-y |
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| author | Yaqiong Zhang Bo Li Wanhong Gu Linna Fan Xiaofan Wang Meifen Xu Minqi Zhu Chong Jin |
| author_facet | Yaqiong Zhang Bo Li Wanhong Gu Linna Fan Xiaofan Wang Meifen Xu Minqi Zhu Chong Jin |
| author_sort | Yaqiong Zhang |
| collection | DOAJ |
| description | Abstract Background A recent study revealed the oncogenic role of box C/D small nucleolar RNA 52 (SNORD52) in hepatocellular carcinoma (HCC) by facilitating the aggressive phenotypes of hepatoma cells. However, the potential role of exosomal SNORD52 in macrophage polarization during HCC progression remains poorly understood. Methods Exosomes were isolated from hepatoma cells. Western blotting and flow cytometry were performed to determine the levels of M2 macrophage polarization markers. SNORD52 expression was assessed using qRT-PCR. The levels of JAK2/STAT6 pathway-related proteins were analyzed using western blotting. Results SNORD52 was enriched in exosomes derived from hepatoma cells and in plasma samples from patients with HCC. Hepatoma cell-derived exosomal SNORD52 was internalized by THP-1 macrophages. SNORD52 overexpression increased the levels of M2 macrophage polarization markers and JAK2/STAT6 pathway-related proteins Additionally, hepatoma cell-derived exosomal SNORD52 interacted with the JAK2/STAT6 pathway to mediate M2 macrophage polarization. Conclusions Our findings revealed that hepatoma cell-derived exosomal SNORD52 induces M2 macrophage polarization by activating the JAK2/STAT6 pathway. |
| format | Article |
| id | doaj-art-eed0b42cb1194289b4b8d5f84fb5cb0c |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-eed0b42cb1194289b4b8d5f84fb5cb0c2025-01-19T12:29:11ZengSpringerDiscover Oncology2730-60112025-01-0116111310.1007/s12672-024-01700-yHepatoma cell-derived exosomal SNORD52 mediates M2 macrophage polarization by activating the JAK2/STAT6 pathwayYaqiong Zhang0Bo Li1Wanhong Gu2Linna Fan3Xiaofan Wang4Meifen Xu5Minqi Zhu6Chong Jin7Department of Clinical Laboratory, Taizhou Central Hospital (Taizhou University Hospital)Department of Ultrasound, Taizhou Hospital, Zhejiang University, Taizhou Enze Medical Center (Group)Department of Clinical Laboratory, Taizhou Central Hospital (Taizhou University Hospital)School of Laboratory Medicine and Life Sciences, Wenzhou Medical UniversityDepartment of Clinical Laboratory, Taizhou Central Hospital (Taizhou University Hospital)Department of Clinical Laboratory, Taizhou Central Hospital (Taizhou University Hospital)Department of Clinical Laboratory, Taizhou Central Hospital (Taizhou University Hospital)Department of Hepatobiliary Pancreatic Splenic Surgery, Taizhou Central Hospital (Taizhou University Hospital)Abstract Background A recent study revealed the oncogenic role of box C/D small nucleolar RNA 52 (SNORD52) in hepatocellular carcinoma (HCC) by facilitating the aggressive phenotypes of hepatoma cells. However, the potential role of exosomal SNORD52 in macrophage polarization during HCC progression remains poorly understood. Methods Exosomes were isolated from hepatoma cells. Western blotting and flow cytometry were performed to determine the levels of M2 macrophage polarization markers. SNORD52 expression was assessed using qRT-PCR. The levels of JAK2/STAT6 pathway-related proteins were analyzed using western blotting. Results SNORD52 was enriched in exosomes derived from hepatoma cells and in plasma samples from patients with HCC. Hepatoma cell-derived exosomal SNORD52 was internalized by THP-1 macrophages. SNORD52 overexpression increased the levels of M2 macrophage polarization markers and JAK2/STAT6 pathway-related proteins Additionally, hepatoma cell-derived exosomal SNORD52 interacted with the JAK2/STAT6 pathway to mediate M2 macrophage polarization. Conclusions Our findings revealed that hepatoma cell-derived exosomal SNORD52 induces M2 macrophage polarization by activating the JAK2/STAT6 pathway.https://doi.org/10.1007/s12672-024-01700-yHepatocellular carcinomaBox C/D small nucleolar RNA 52ExosomeMacrophagePolarization |
| spellingShingle | Yaqiong Zhang Bo Li Wanhong Gu Linna Fan Xiaofan Wang Meifen Xu Minqi Zhu Chong Jin Hepatoma cell-derived exosomal SNORD52 mediates M2 macrophage polarization by activating the JAK2/STAT6 pathway Discover Oncology Hepatocellular carcinoma Box C/D small nucleolar RNA 52 Exosome Macrophage Polarization |
| title | Hepatoma cell-derived exosomal SNORD52 mediates M2 macrophage polarization by activating the JAK2/STAT6 pathway |
| title_full | Hepatoma cell-derived exosomal SNORD52 mediates M2 macrophage polarization by activating the JAK2/STAT6 pathway |
| title_fullStr | Hepatoma cell-derived exosomal SNORD52 mediates M2 macrophage polarization by activating the JAK2/STAT6 pathway |
| title_full_unstemmed | Hepatoma cell-derived exosomal SNORD52 mediates M2 macrophage polarization by activating the JAK2/STAT6 pathway |
| title_short | Hepatoma cell-derived exosomal SNORD52 mediates M2 macrophage polarization by activating the JAK2/STAT6 pathway |
| title_sort | hepatoma cell derived exosomal snord52 mediates m2 macrophage polarization by activating the jak2 stat6 pathway |
| topic | Hepatocellular carcinoma Box C/D small nucleolar RNA 52 Exosome Macrophage Polarization |
| url | https://doi.org/10.1007/s12672-024-01700-y |
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