Regional alterations in purkinje cell density in patients with autism.

Neuropathological studies, using a variety of techniques, have reported a decrease in Purkinje cell (PC) density in the cerebellum in autism. We have used a systematic sampling technique that significantly reduces experimenter bias and variance to estimate PC densities in the postmortem brains of ei...

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Main Authors: Jerry Skefos, Christopher Cummings, Katelyn Enzer, Jarrod Holiday, Katrina Weed, Ezra Levy, Tarik Yuce, Thomas Kemper, Margaret Bauman
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0081255&type=printable
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author Jerry Skefos
Christopher Cummings
Katelyn Enzer
Jarrod Holiday
Katrina Weed
Ezra Levy
Tarik Yuce
Thomas Kemper
Margaret Bauman
author_facet Jerry Skefos
Christopher Cummings
Katelyn Enzer
Jarrod Holiday
Katrina Weed
Ezra Levy
Tarik Yuce
Thomas Kemper
Margaret Bauman
author_sort Jerry Skefos
collection DOAJ
description Neuropathological studies, using a variety of techniques, have reported a decrease in Purkinje cell (PC) density in the cerebellum in autism. We have used a systematic sampling technique that significantly reduces experimenter bias and variance to estimate PC densities in the postmortem brains of eight clinically well-documented individuals with autism, and eight age- and gender-matched controls. Four cerebellar regions were analyzed: a sensorimotor area comprised of hemispheric lobules IV-VI, crus I & II of the posterior lobe, and lobule X of the flocculonodular lobe. Overall PC density was thus estimated using data from all three cerebellar lobes and was found to be lower in the cases with autism as compared to controls, an effect that was most prominent in crus I and II (p<0.05). Lobule X demonstrated a trend towards lower PC density in only the males with autism (p = 0.05). Brain weight, a correlate of tissue volume, was found to significantly contribute to the lower lobule X PC density observed in males with autism, but not to the finding of lower PC density in crus I & II. Therefore, lower crus I & II PC density in autism is more likely due to a lower number of PCs. The PC density in lobule X was found to correlate with the ADI-R measure of the patient's use of social eye contact (R² = -0.75, p = 0.012). These findings support the hypothesis that abnormal PC density may contribute to selected clinical features of the autism phenotype.
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spelling doaj-art-eec9cf602d1343f786064e28eb9c1e132025-08-20T02:15:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8125510.1371/journal.pone.0081255Regional alterations in purkinje cell density in patients with autism.Jerry SkefosChristopher CummingsKatelyn EnzerJarrod HolidayKatrina WeedEzra LevyTarik YuceThomas KemperMargaret BaumanNeuropathological studies, using a variety of techniques, have reported a decrease in Purkinje cell (PC) density in the cerebellum in autism. We have used a systematic sampling technique that significantly reduces experimenter bias and variance to estimate PC densities in the postmortem brains of eight clinically well-documented individuals with autism, and eight age- and gender-matched controls. Four cerebellar regions were analyzed: a sensorimotor area comprised of hemispheric lobules IV-VI, crus I & II of the posterior lobe, and lobule X of the flocculonodular lobe. Overall PC density was thus estimated using data from all three cerebellar lobes and was found to be lower in the cases with autism as compared to controls, an effect that was most prominent in crus I and II (p<0.05). Lobule X demonstrated a trend towards lower PC density in only the males with autism (p = 0.05). Brain weight, a correlate of tissue volume, was found to significantly contribute to the lower lobule X PC density observed in males with autism, but not to the finding of lower PC density in crus I & II. Therefore, lower crus I & II PC density in autism is more likely due to a lower number of PCs. The PC density in lobule X was found to correlate with the ADI-R measure of the patient's use of social eye contact (R² = -0.75, p = 0.012). These findings support the hypothesis that abnormal PC density may contribute to selected clinical features of the autism phenotype.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0081255&type=printable
spellingShingle Jerry Skefos
Christopher Cummings
Katelyn Enzer
Jarrod Holiday
Katrina Weed
Ezra Levy
Tarik Yuce
Thomas Kemper
Margaret Bauman
Regional alterations in purkinje cell density in patients with autism.
PLoS ONE
title Regional alterations in purkinje cell density in patients with autism.
title_full Regional alterations in purkinje cell density in patients with autism.
title_fullStr Regional alterations in purkinje cell density in patients with autism.
title_full_unstemmed Regional alterations in purkinje cell density in patients with autism.
title_short Regional alterations in purkinje cell density in patients with autism.
title_sort regional alterations in purkinje cell density in patients with autism
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0081255&type=printable
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