Nucleolar sequestration of cannabinoid type-2 receptors in triple-negative breast cancer cells.
Multiple investigations have shown that the different types of cannabinoids, phytocannabinoids, synthetic cannabinoids, and endocannabinoids, possess antiproliferative and anticancer properties. The cannabinoid type-2 receptor (CB2R) has been proposed as a central player in tumor progression and has...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0323554 |
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| author | Linley P Prado-Celis Rodrigo Zamora-Cárdenas Javier Alamilla Enrique A Sánchez-Pastor Tania Ferrer Eloy G Moreno-Galindo Ricardo A Navarro-Polanco |
| author_facet | Linley P Prado-Celis Rodrigo Zamora-Cárdenas Javier Alamilla Enrique A Sánchez-Pastor Tania Ferrer Eloy G Moreno-Galindo Ricardo A Navarro-Polanco |
| author_sort | Linley P Prado-Celis |
| collection | DOAJ |
| description | Multiple investigations have shown that the different types of cannabinoids, phytocannabinoids, synthetic cannabinoids, and endocannabinoids, possess antiproliferative and anticancer properties. The cannabinoid type-2 receptor (CB2R) has been proposed as a central player in tumor progression and has been correlated with the aggressiveness of breast cancer. Using immunocytochemistry and confocal microscopy, in the present work, we studied the expression level and subcellular localization of CB2R in two human triple-negative breast cancer (TNBC) cell lines, corresponding to early (stage I, HCC-1395) and metastatic (MDA-MB-231) stages, and they were compared with a non-tumoral mammary epithelial cell line (MCF-10A). We found that although CB2R was detected at the plasma membrane, it was mainly localized intracellularly, with ~40-fold higher expression in both TNBC cell lines than in MCF-10A (P < 0.0001). Notably, double staining with DAPI or with the nucleoli-specific fluorescent marker (3xnls-mTurquoise2) showed that most of the CB2R overexpressed in the nucleoli of cancer cells. This finding is supported by the fact that CB2R expression was markedly lower in mitotic cells compared to interphase cells (P < 0.0001). Interestingly, exposure of cancer cells to the specific agonist HU-308 reversed the nucleolar sequestration of CB2R while increasing the presence of the receptor in the nucleoplasm and cytoplasm (P < 0.0001). In addition, we found that this agonist reduced both the cell migration (P < 0.05-0.0001) and proliferation (P < 0.001) of TNBC cells. It remains to determine the function and signaling ability of CB2R in the nucleolus. Although our study only includes cell lines (tumoral and non-tumoral), we consider that this feature of nucleolar sequestration of CB2R could be a potential diagnostic marker for TNBC from the early stage. |
| format | Article |
| id | doaj-art-eec8b1eeab06487d895fe8022e24d2ab |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-eec8b1eeab06487d895fe8022e24d2ab2025-08-20T03:47:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01205e032355410.1371/journal.pone.0323554Nucleolar sequestration of cannabinoid type-2 receptors in triple-negative breast cancer cells.Linley P Prado-CelisRodrigo Zamora-CárdenasJavier AlamillaEnrique A Sánchez-PastorTania FerrerEloy G Moreno-GalindoRicardo A Navarro-PolancoMultiple investigations have shown that the different types of cannabinoids, phytocannabinoids, synthetic cannabinoids, and endocannabinoids, possess antiproliferative and anticancer properties. The cannabinoid type-2 receptor (CB2R) has been proposed as a central player in tumor progression and has been correlated with the aggressiveness of breast cancer. Using immunocytochemistry and confocal microscopy, in the present work, we studied the expression level and subcellular localization of CB2R in two human triple-negative breast cancer (TNBC) cell lines, corresponding to early (stage I, HCC-1395) and metastatic (MDA-MB-231) stages, and they were compared with a non-tumoral mammary epithelial cell line (MCF-10A). We found that although CB2R was detected at the plasma membrane, it was mainly localized intracellularly, with ~40-fold higher expression in both TNBC cell lines than in MCF-10A (P < 0.0001). Notably, double staining with DAPI or with the nucleoli-specific fluorescent marker (3xnls-mTurquoise2) showed that most of the CB2R overexpressed in the nucleoli of cancer cells. This finding is supported by the fact that CB2R expression was markedly lower in mitotic cells compared to interphase cells (P < 0.0001). Interestingly, exposure of cancer cells to the specific agonist HU-308 reversed the nucleolar sequestration of CB2R while increasing the presence of the receptor in the nucleoplasm and cytoplasm (P < 0.0001). In addition, we found that this agonist reduced both the cell migration (P < 0.05-0.0001) and proliferation (P < 0.001) of TNBC cells. It remains to determine the function and signaling ability of CB2R in the nucleolus. Although our study only includes cell lines (tumoral and non-tumoral), we consider that this feature of nucleolar sequestration of CB2R could be a potential diagnostic marker for TNBC from the early stage.https://doi.org/10.1371/journal.pone.0323554 |
| spellingShingle | Linley P Prado-Celis Rodrigo Zamora-Cárdenas Javier Alamilla Enrique A Sánchez-Pastor Tania Ferrer Eloy G Moreno-Galindo Ricardo A Navarro-Polanco Nucleolar sequestration of cannabinoid type-2 receptors in triple-negative breast cancer cells. PLoS ONE |
| title | Nucleolar sequestration of cannabinoid type-2 receptors in triple-negative breast cancer cells. |
| title_full | Nucleolar sequestration of cannabinoid type-2 receptors in triple-negative breast cancer cells. |
| title_fullStr | Nucleolar sequestration of cannabinoid type-2 receptors in triple-negative breast cancer cells. |
| title_full_unstemmed | Nucleolar sequestration of cannabinoid type-2 receptors in triple-negative breast cancer cells. |
| title_short | Nucleolar sequestration of cannabinoid type-2 receptors in triple-negative breast cancer cells. |
| title_sort | nucleolar sequestration of cannabinoid type 2 receptors in triple negative breast cancer cells |
| url | https://doi.org/10.1371/journal.pone.0323554 |
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