Analysis of microbial community differences during wastewater treatment of gelatin production plants based on high-throughput sequencing
In order to understand the structural differences of bacterial flora in the treatment process of gelatin processing wastewater, the 16S rRNA V3-V4 region of bacterial community in four sampling points of wastewater (initial settling water, aerobic water, secondary sedimentary sludge and discharge wa...
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Editorial Department of China Brewing
2024-07-01
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| Series: | Zhongguo niangzao |
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| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-7-86.pdf |
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| author | XU Na, REN Haiwei, YAN Jin, ZHAO Linglong, TANG Duoli, TAO Tao |
| author_facet | XU Na, REN Haiwei, YAN Jin, ZHAO Linglong, TANG Duoli, TAO Tao |
| author_sort | XU Na, REN Haiwei, YAN Jin, ZHAO Linglong, TANG Duoli, TAO Tao |
| collection | DOAJ |
| description | In order to understand the structural differences of bacterial flora in the treatment process of gelatin processing wastewater, the 16S rRNA V3-V4 region of bacterial community in four sampling points of wastewater (initial settling water, aerobic water, secondary sedimentary sludge and discharge water) were investigated by high throughput sequencing using Illumina Hiseq sequencing platform. The results demonstrated that the dominant bacteria of initial settling water were Proteobacteria (69.62%) and Firmicutes (29.77%) at phylum level, Uncultured bacterium WS6 (34.48%, 22.75%) and Proteobacteria (24.85%, 22.88%) were dominated in aerobic water and secondary sedimentary sludge, Saccharibacteria (8.89%) and Proteobacteria (16.49%) were dominated in discharge water. At the genus level, the dominant bacteria of initial settling water were Hydrogenophaga (37.57%), while the dominant bacteria in aerobic wastewater and secondary sedimentary sludge were uncultured bacterium p WS6 (34.48%, 22.76%), and the dominant bacteria of discharge water was uncultured bacterium p Saccharibacteria (28.89%). There were significant differences of bacterial community diversity of four wastewater samples. The bacterial communities structure in wastewater treatment system was significantly correlated with water quality environmental factors (P<0.05). Rheinheimera was positively correlated with pH and the content of Ca<sup>2+</sup>, ammonia nitrogen (P<0.05). Alkalibacterium was positively correlated with the content of total nitrogen and chemical oxygen demand (P<0.05). In summary, the environmental factors of wastewater were closely related to the structure of microbial communities, and the functions of key bacterial community in wastewater treatment was ascertained by the established relationship between environmental factors and microbial communities. |
| format | Article |
| id | doaj-art-eec2b24959bd4e8fbfb503a79e38ff2d |
| institution | DOAJ |
| issn | 0254-5071 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-eec2b24959bd4e8fbfb503a79e38ff2d2025-08-20T02:55:02ZengEditorial Department of China BrewingZhongguo niangzao0254-50712024-07-01437869210.11882/j.issn.0254-5071.2024.07.013Analysis of microbial community differences during wastewater treatment of gelatin production plants based on high-throughput sequencingXU Na, REN Haiwei, YAN Jin, ZHAO Linglong, TANG Duoli, TAO Tao01. Lanzhou Qilihe District Center for Disease Control and Prevention, Lanzhou 730050, China; ;2. College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, ChinaIn order to understand the structural differences of bacterial flora in the treatment process of gelatin processing wastewater, the 16S rRNA V3-V4 region of bacterial community in four sampling points of wastewater (initial settling water, aerobic water, secondary sedimentary sludge and discharge water) were investigated by high throughput sequencing using Illumina Hiseq sequencing platform. The results demonstrated that the dominant bacteria of initial settling water were Proteobacteria (69.62%) and Firmicutes (29.77%) at phylum level, Uncultured bacterium WS6 (34.48%, 22.75%) and Proteobacteria (24.85%, 22.88%) were dominated in aerobic water and secondary sedimentary sludge, Saccharibacteria (8.89%) and Proteobacteria (16.49%) were dominated in discharge water. At the genus level, the dominant bacteria of initial settling water were Hydrogenophaga (37.57%), while the dominant bacteria in aerobic wastewater and secondary sedimentary sludge were uncultured bacterium p WS6 (34.48%, 22.76%), and the dominant bacteria of discharge water was uncultured bacterium p Saccharibacteria (28.89%). There were significant differences of bacterial community diversity of four wastewater samples. The bacterial communities structure in wastewater treatment system was significantly correlated with water quality environmental factors (P<0.05). Rheinheimera was positively correlated with pH and the content of Ca<sup>2+</sup>, ammonia nitrogen (P<0.05). Alkalibacterium was positively correlated with the content of total nitrogen and chemical oxygen demand (P<0.05). In summary, the environmental factors of wastewater were closely related to the structure of microbial communities, and the functions of key bacterial community in wastewater treatment was ascertained by the established relationship between environmental factors and microbial communities.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-7-86.pdfgelatin processing wastewater|bacterial community|water quality|high-throughput sequencing|environmental factor |
| spellingShingle | XU Na, REN Haiwei, YAN Jin, ZHAO Linglong, TANG Duoli, TAO Tao Analysis of microbial community differences during wastewater treatment of gelatin production plants based on high-throughput sequencing Zhongguo niangzao gelatin processing wastewater|bacterial community|water quality|high-throughput sequencing|environmental factor |
| title | Analysis of microbial community differences during wastewater treatment of gelatin production plants based on high-throughput sequencing |
| title_full | Analysis of microbial community differences during wastewater treatment of gelatin production plants based on high-throughput sequencing |
| title_fullStr | Analysis of microbial community differences during wastewater treatment of gelatin production plants based on high-throughput sequencing |
| title_full_unstemmed | Analysis of microbial community differences during wastewater treatment of gelatin production plants based on high-throughput sequencing |
| title_short | Analysis of microbial community differences during wastewater treatment of gelatin production plants based on high-throughput sequencing |
| title_sort | analysis of microbial community differences during wastewater treatment of gelatin production plants based on high throughput sequencing |
| topic | gelatin processing wastewater|bacterial community|water quality|high-throughput sequencing|environmental factor |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-7-86.pdf |
| work_keys_str_mv | AT xunarenhaiweiyanjinzhaolinglongtangduolitaotao analysisofmicrobialcommunitydifferencesduringwastewatertreatmentofgelatinproductionplantsbasedonhighthroughputsequencing |