Virus transmission frequencies in the pine root rot pathogen Heterobasidion annosum
The combined use of Heterobasidion partitiviruses 13 and 15 (HetPV13-an1 and HetPV15-pa1) is considered a promising biocontrol approach against Heterobasidion root and butt rot. In a previous study, the transmission frequency of HetPV15-pa1 was found to be higher from a double partitivirus-infected...
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Elsevier
2024-12-01
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| Series: | Virus Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168170224001606 |
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| author | Elina Roininen Eeva Johanna Vainio Suvi Sutela Anna Poimala Muhammad Kashif Tuula Piri Jarkko Hantula |
| author_facet | Elina Roininen Eeva Johanna Vainio Suvi Sutela Anna Poimala Muhammad Kashif Tuula Piri Jarkko Hantula |
| author_sort | Elina Roininen |
| collection | DOAJ |
| description | The combined use of Heterobasidion partitiviruses 13 and 15 (HetPV13-an1 and HetPV15-pa1) is considered a promising biocontrol approach against Heterobasidion root and butt rot. In a previous study, the transmission frequency of HetPV15-pa1 was found to be higher from a double partitivirus-infected donor than from a single partitivirus-infected donor. In this study, we included a wider array of recipient isolates to assess whether the phenomenon is widespread across different host strains and conducted transmission experiments on artificial media (in vitro) using a total of 45 different H. annosum donor-recipient pairs. In addition to investigating whether double partitivirus infection improves the transmission of HetPV13-an1 and HetPV15-pa1, we examined for the first time how efficiently co-infecting ssRNA viruses are concomitantly transmitted with the partitiviruses, and whether pre-existing ssRNA viruses in the recipients affect virus transmission. Generally, the transmission rates of HetPV13-an1 and HetPV15-pa1 were high from both single partitivirus-infected and double partitivirus-infected donors to most of the H. annosum recipient strains, with few exceptions. However, in contrast to previous experiments, the transmission frequency was not higher from the double partitivirus-infected donors. Also, ourmiavirus was transmitted between H. annosum strains, but the presence of another ourmiavirus in the recipient might affect the efficacy. |
| format | Article |
| id | doaj-art-eec273e837834302bca4fb29dfc7c0cc |
| institution | DOAJ |
| issn | 1872-7492 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Virus Research |
| spelling | doaj-art-eec273e837834302bca4fb29dfc7c0cc2025-08-20T02:52:26ZengElsevierVirus Research1872-74922024-12-0135019946710.1016/j.virusres.2024.199467Virus transmission frequencies in the pine root rot pathogen Heterobasidion annosumElina Roininen0Eeva Johanna Vainio1Suvi Sutela2Anna Poimala3Muhammad Kashif4Tuula Piri5Jarkko Hantula6University of Helsinki, Viikinkaari 1, Helsinki FI-00790, Finland; Natural Resources Institute Finland (Luke), Latokartanonkaari 9, Helsinki FI-00790, Finland; Corresponding author.Natural Resources Institute Finland (Luke), Latokartanonkaari 9, Helsinki FI-00790, FinlandNatural Resources Institute Finland (Luke), Latokartanonkaari 9, Helsinki FI-00790, FinlandNatural Resources Institute Finland (Luke), Latokartanonkaari 9, Helsinki FI-00790, FinlandNatural Resources Institute Finland (Luke), Latokartanonkaari 9, Helsinki FI-00790, FinlandNatural Resources Institute Finland (Luke), Latokartanonkaari 9, Helsinki FI-00790, FinlandNatural Resources Institute Finland (Luke), Latokartanonkaari 9, Helsinki FI-00790, FinlandThe combined use of Heterobasidion partitiviruses 13 and 15 (HetPV13-an1 and HetPV15-pa1) is considered a promising biocontrol approach against Heterobasidion root and butt rot. In a previous study, the transmission frequency of HetPV15-pa1 was found to be higher from a double partitivirus-infected donor than from a single partitivirus-infected donor. In this study, we included a wider array of recipient isolates to assess whether the phenomenon is widespread across different host strains and conducted transmission experiments on artificial media (in vitro) using a total of 45 different H. annosum donor-recipient pairs. In addition to investigating whether double partitivirus infection improves the transmission of HetPV13-an1 and HetPV15-pa1, we examined for the first time how efficiently co-infecting ssRNA viruses are concomitantly transmitted with the partitiviruses, and whether pre-existing ssRNA viruses in the recipients affect virus transmission. Generally, the transmission rates of HetPV13-an1 and HetPV15-pa1 were high from both single partitivirus-infected and double partitivirus-infected donors to most of the H. annosum recipient strains, with few exceptions. However, in contrast to previous experiments, the transmission frequency was not higher from the double partitivirus-infected donors. Also, ourmiavirus was transmitted between H. annosum strains, but the presence of another ourmiavirus in the recipient might affect the efficacy.http://www.sciencedirect.com/science/article/pii/S0168170224001606HeterobasidionTransmissionPartitivirusOurmiavirusMitovirusCo-infection |
| spellingShingle | Elina Roininen Eeva Johanna Vainio Suvi Sutela Anna Poimala Muhammad Kashif Tuula Piri Jarkko Hantula Virus transmission frequencies in the pine root rot pathogen Heterobasidion annosum Virus Research Heterobasidion Transmission Partitivirus Ourmiavirus Mitovirus Co-infection |
| title | Virus transmission frequencies in the pine root rot pathogen Heterobasidion annosum |
| title_full | Virus transmission frequencies in the pine root rot pathogen Heterobasidion annosum |
| title_fullStr | Virus transmission frequencies in the pine root rot pathogen Heterobasidion annosum |
| title_full_unstemmed | Virus transmission frequencies in the pine root rot pathogen Heterobasidion annosum |
| title_short | Virus transmission frequencies in the pine root rot pathogen Heterobasidion annosum |
| title_sort | virus transmission frequencies in the pine root rot pathogen heterobasidion annosum |
| topic | Heterobasidion Transmission Partitivirus Ourmiavirus Mitovirus Co-infection |
| url | http://www.sciencedirect.com/science/article/pii/S0168170224001606 |
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