High-sensitivity colorimetric sensor based on oxidase-like Mn3O4 nanozyme for Cys detection
Cysteine (Cys) is essential for numerous physiological processes and play a vital role in disease diagnostics, underscoring the necessity for effective and sensitive detection techniques for Cys. In this research, we introduce an ultrasensitive colorimetric approach based on oxidase-like Mn3O4 nanoz...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | Sensors and Actuators Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053925000165 |
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| author | Junting Liang Dihan Duan Lubing Sun Jinming Li Mengke Wang Ziyi Chang Rick F. Thorne Chuanliang Chen Demin Duan |
| author_facet | Junting Liang Dihan Duan Lubing Sun Jinming Li Mengke Wang Ziyi Chang Rick F. Thorne Chuanliang Chen Demin Duan |
| author_sort | Junting Liang |
| collection | DOAJ |
| description | Cysteine (Cys) is essential for numerous physiological processes and play a vital role in disease diagnostics, underscoring the necessity for effective and sensitive detection techniques for Cys. In this research, we introduce an ultrasensitive colorimetric approach based on oxidase-like Mn3O4 nanozymes. The nanozymes were synthesized through a straightforward wet chemical method and displayed a flower-like globular morphology, showing remarkable oxidase-like catalytic performance with a low Km value of 0.1896 mM and a high Vmax of 2.8 × 10⁻⁷ M s⁻¹. By leveraging the outstanding catalytic activities of Mn3O4 nanozymes and optimizing the TMB concentration, we developed a novel and sensitive colorimetric sensor for Cys detection. Experimental results indicated a detection range for Cys between 0.5 and 45 μM, with a lower limit of detection (LOD) of 0.02636 μM. This established method for detecting Cys reveals significant promise for the advancement of effective sensing systems. |
| format | Article |
| id | doaj-art-eec23790b7fb4c0fb7851291f4ddc0d3 |
| institution | Kabale University |
| issn | 2666-0539 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensors and Actuators Reports |
| spelling | doaj-art-eec23790b7fb4c0fb7851291f4ddc0d32025-02-08T05:01:10ZengElsevierSensors and Actuators Reports2666-05392025-06-019100296High-sensitivity colorimetric sensor based on oxidase-like Mn3O4 nanozyme for Cys detectionJunting Liang0Dihan Duan1Lubing Sun2Jinming Li3Mengke Wang4Ziyi Chang5Rick F. Thorne6Chuanliang Chen7Demin Duan8Clinical Bioinformatics Experimental Center, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, Henan, ChinaNanozyme Laboratory in Zhongyuan, Henan Academy of Innovations in Medical Science, Zhengzhou 451163, Henan, ChinaClinical Bioinformatics Experimental Center, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, Henan, ChinaTranslational Research Institute of Henan Provincial People's Hospital, Zhengzhou University, Zhengzhou 450003, Henan, ChinaNanozyme Laboratory in Zhongyuan, Henan Academy of Innovations in Medical Science, Zhengzhou 451163, Henan, ChinaNanozyme Laboratory in Zhongyuan, Henan Academy of Innovations in Medical Science, Zhengzhou 451163, Henan, ChinaTranslational Research Institute of Henan Provincial People's Hospital, Zhengzhou University, Zhengzhou 450003, Henan, ChinaClinical Bioinformatics Experimental Center, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, Henan, China; Corresponding authors.Nanozyme Laboratory in Zhongyuan, Henan Academy of Innovations in Medical Science, Zhengzhou 451163, Henan, China; CAS Engineering Laboratory for Nanozyme, National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; Corresponding authors.Cysteine (Cys) is essential for numerous physiological processes and play a vital role in disease diagnostics, underscoring the necessity for effective and sensitive detection techniques for Cys. In this research, we introduce an ultrasensitive colorimetric approach based on oxidase-like Mn3O4 nanozymes. The nanozymes were synthesized through a straightforward wet chemical method and displayed a flower-like globular morphology, showing remarkable oxidase-like catalytic performance with a low Km value of 0.1896 mM and a high Vmax of 2.8 × 10⁻⁷ M s⁻¹. By leveraging the outstanding catalytic activities of Mn3O4 nanozymes and optimizing the TMB concentration, we developed a novel and sensitive colorimetric sensor for Cys detection. Experimental results indicated a detection range for Cys between 0.5 and 45 μM, with a lower limit of detection (LOD) of 0.02636 μM. This established method for detecting Cys reveals significant promise for the advancement of effective sensing systems.http://www.sciencedirect.com/science/article/pii/S2666053925000165Oxidase-like nanozymeMn3O4Colorimetric sensorCysteine |
| spellingShingle | Junting Liang Dihan Duan Lubing Sun Jinming Li Mengke Wang Ziyi Chang Rick F. Thorne Chuanliang Chen Demin Duan High-sensitivity colorimetric sensor based on oxidase-like Mn3O4 nanozyme for Cys detection Sensors and Actuators Reports Oxidase-like nanozyme Mn3O4 Colorimetric sensor Cysteine |
| title | High-sensitivity colorimetric sensor based on oxidase-like Mn3O4 nanozyme for Cys detection |
| title_full | High-sensitivity colorimetric sensor based on oxidase-like Mn3O4 nanozyme for Cys detection |
| title_fullStr | High-sensitivity colorimetric sensor based on oxidase-like Mn3O4 nanozyme for Cys detection |
| title_full_unstemmed | High-sensitivity colorimetric sensor based on oxidase-like Mn3O4 nanozyme for Cys detection |
| title_short | High-sensitivity colorimetric sensor based on oxidase-like Mn3O4 nanozyme for Cys detection |
| title_sort | high sensitivity colorimetric sensor based on oxidase like mn3o4 nanozyme for cys detection |
| topic | Oxidase-like nanozyme Mn3O4 Colorimetric sensor Cysteine |
| url | http://www.sciencedirect.com/science/article/pii/S2666053925000165 |
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