A Fluorescent Reporter Virus Toolkit for Interrogating Enterovirus Biology and Host Interactions
Enteroviruses are a group of highly prevalent human pathogens responsible for a wide range of illnesses, ranging from common cold symptoms to life-threatening diseases. A deep understanding of enterovirus biology, evolution, and host interaction is required for the development of effective vaccines...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/17/6/796 |
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| author | Mireya Martínez-Pérez Sebastian Velandia-Álvarez Cristina Vidal-Verdú Beatriz Álvarez-Rodríguez Ron Geller |
| author_facet | Mireya Martínez-Pérez Sebastian Velandia-Álvarez Cristina Vidal-Verdú Beatriz Álvarez-Rodríguez Ron Geller |
| author_sort | Mireya Martínez-Pérez |
| collection | DOAJ |
| description | Enteroviruses are a group of highly prevalent human pathogens responsible for a wide range of illnesses, ranging from common cold symptoms to life-threatening diseases. A deep understanding of enterovirus biology, evolution, and host interaction is required for the development of effective vaccines and antivirals. Recombinant reporter viruses encoding luminescent or fluorescent proteins have been developed to facilitate such investigation. In this work, using coxsackievirus B3 as our model, we analyze how the insertion of fluorescent reporter genes at three distinct sites within the viral polyprotein affects viral fitness, identifying the most tolerant site for reporter insertion. We then describe a set of experimental workflows for measuring viral fitness, sera neutralization, antiviral efficacy, and recombination using fluorescent reporter viruses. The high homology between different enteroviruses suggests these assays can be readily adapted to study additional members of this medically and economically relevant group of viruses. |
| format | Article |
| id | doaj-art-eec20270e7cb42c9924e2d70b2ad8a4b |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-eec20270e7cb42c9924e2d70b2ad8a4b2025-08-20T03:26:56ZengMDPI AGViruses1999-49152025-05-0117679610.3390/v17060796A Fluorescent Reporter Virus Toolkit for Interrogating Enterovirus Biology and Host InteractionsMireya Martínez-Pérez0Sebastian Velandia-Álvarez1Cristina Vidal-Verdú2Beatriz Álvarez-Rodríguez3Ron Geller4Institute for Integrative Systems Biology (I<sup>2</sup>SysBio), Universitat de València—Consejo Superior de Investigaciones Científicas (UV-CSIC), 46980 Valencia, SpainInstitute for Integrative Systems Biology (I<sup>2</sup>SysBio), Universitat de València—Consejo Superior de Investigaciones Científicas (UV-CSIC), 46980 Valencia, SpainInstitute for Integrative Systems Biology (I<sup>2</sup>SysBio), Universitat de València—Consejo Superior de Investigaciones Científicas (UV-CSIC), 46980 Valencia, SpainInstitute for Integrative Systems Biology (I<sup>2</sup>SysBio), Universitat de València—Consejo Superior de Investigaciones Científicas (UV-CSIC), 46980 Valencia, SpainInstitute for Integrative Systems Biology (I<sup>2</sup>SysBio), Universitat de València—Consejo Superior de Investigaciones Científicas (UV-CSIC), 46980 Valencia, SpainEnteroviruses are a group of highly prevalent human pathogens responsible for a wide range of illnesses, ranging from common cold symptoms to life-threatening diseases. A deep understanding of enterovirus biology, evolution, and host interaction is required for the development of effective vaccines and antivirals. Recombinant reporter viruses encoding luminescent or fluorescent proteins have been developed to facilitate such investigation. In this work, using coxsackievirus B3 as our model, we analyze how the insertion of fluorescent reporter genes at three distinct sites within the viral polyprotein affects viral fitness, identifying the most tolerant site for reporter insertion. We then describe a set of experimental workflows for measuring viral fitness, sera neutralization, antiviral efficacy, and recombination using fluorescent reporter viruses. The high homology between different enteroviruses suggests these assays can be readily adapted to study additional members of this medically and economically relevant group of viruses.https://www.mdpi.com/1999-4915/17/6/796enterovirusescoxsackievirus B3reporter assaysrecombinationviral fitnessneutralizing antibodies |
| spellingShingle | Mireya Martínez-Pérez Sebastian Velandia-Álvarez Cristina Vidal-Verdú Beatriz Álvarez-Rodríguez Ron Geller A Fluorescent Reporter Virus Toolkit for Interrogating Enterovirus Biology and Host Interactions Viruses enteroviruses coxsackievirus B3 reporter assays recombination viral fitness neutralizing antibodies |
| title | A Fluorescent Reporter Virus Toolkit for Interrogating Enterovirus Biology and Host Interactions |
| title_full | A Fluorescent Reporter Virus Toolkit for Interrogating Enterovirus Biology and Host Interactions |
| title_fullStr | A Fluorescent Reporter Virus Toolkit for Interrogating Enterovirus Biology and Host Interactions |
| title_full_unstemmed | A Fluorescent Reporter Virus Toolkit for Interrogating Enterovirus Biology and Host Interactions |
| title_short | A Fluorescent Reporter Virus Toolkit for Interrogating Enterovirus Biology and Host Interactions |
| title_sort | fluorescent reporter virus toolkit for interrogating enterovirus biology and host interactions |
| topic | enteroviruses coxsackievirus B3 reporter assays recombination viral fitness neutralizing antibodies |
| url | https://www.mdpi.com/1999-4915/17/6/796 |
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