Nuclear factor I-C regulates intramembranous bone formation via control of FGF signalling
Our previous studies indicate that NFI-C is essential for tooth root development and endochondral ossification. However, its exact role in calvarial intramembranous bone formation remains unclear. In this study, we demonstrate that the disruption of the Nfic gene leads to defects in intramembranous...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025001690 |
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| author | Jieun Lee Joo-Cheol Park Heung‐Joong Kim Hyun Sook Bae Dong-Seol Lee |
| author_facet | Jieun Lee Joo-Cheol Park Heung‐Joong Kim Hyun Sook Bae Dong-Seol Lee |
| author_sort | Jieun Lee |
| collection | DOAJ |
| description | Our previous studies indicate that NFI-C is essential for tooth root development and endochondral ossification. However, its exact role in calvarial intramembranous bone formation remains unclear. In this study, we demonstrate that the disruption of the Nfic gene leads to defects in intramembranous bone formation, characterized by decreased osteogenic proliferative activity and reduced osteoblast differentiation during postnatal osteogenesis. Additionally, Nfic-deficient mice exhibited incomplete suture closure, although Nfic disruption did not affect prenatal calvarial bone development. We found that the expression levels of Fgfr1 and Fgfr2 were reduced in the primary calvarial mesenchymal cells of Nfic-deficient mice. In contrast, NFI-C overexpression in human bone marrow stromal cells (hBMSCs) significantly increased the expression of these factors. Furthermore, NFI-C regulates FGFR1 expression by directly binding to its promoter. These results indicate that NFI-C is crucial in regulating calvarial bone formation and suture closure by controlling Fgfr1 expression and cellular proliferation. |
| format | Article |
| id | doaj-art-eec138237837464288326c0b684b37db |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-eec138237837464288326c0b684b37db2025-02-02T05:28:12ZengElsevierHeliyon2405-84402025-01-01112e41789Nuclear factor I-C regulates intramembranous bone formation via control of FGF signallingJieun Lee0Joo-Cheol Park1Heung‐Joong Kim2Hyun Sook Bae3Dong-Seol Lee4Department of Oral Hygiene, Namseoul University, Cheonan, Republic of KoreaDepartment of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea; Regenerative Dental Medicine R and D Center, Hysensbio Co., Ltd., Seoul, Republic of KoreaDepartment of Anatomy and Orofacial Development School of Dentistry Chosun University, Dong-gu, Gwangju, Republic of KoreaDepartment of Oral Hygiene, Namseoul University, Cheonan, Republic of Korea; Corresponding author. Department of Oral Hygiene, Namseoul University, Cheonan, 31020, Republic of Korea.Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea; Regenerative Dental Medicine R and D Center, Hysensbio Co., Ltd., Seoul, Republic of Korea; Corresponding author. Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University, 1, Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.Our previous studies indicate that NFI-C is essential for tooth root development and endochondral ossification. However, its exact role in calvarial intramembranous bone formation remains unclear. In this study, we demonstrate that the disruption of the Nfic gene leads to defects in intramembranous bone formation, characterized by decreased osteogenic proliferative activity and reduced osteoblast differentiation during postnatal osteogenesis. Additionally, Nfic-deficient mice exhibited incomplete suture closure, although Nfic disruption did not affect prenatal calvarial bone development. We found that the expression levels of Fgfr1 and Fgfr2 were reduced in the primary calvarial mesenchymal cells of Nfic-deficient mice. In contrast, NFI-C overexpression in human bone marrow stromal cells (hBMSCs) significantly increased the expression of these factors. Furthermore, NFI-C regulates FGFR1 expression by directly binding to its promoter. These results indicate that NFI-C is crucial in regulating calvarial bone formation and suture closure by controlling Fgfr1 expression and cellular proliferation.http://www.sciencedirect.com/science/article/pii/S2405844025001690Nuclear factor I-C (NFI-C)Fibroblast growth factor receptor 1 (FGFR1)Osteogenic progenitor cellsProliferationOsteoblast differentiation |
| spellingShingle | Jieun Lee Joo-Cheol Park Heung‐Joong Kim Hyun Sook Bae Dong-Seol Lee Nuclear factor I-C regulates intramembranous bone formation via control of FGF signalling Heliyon Nuclear factor I-C (NFI-C) Fibroblast growth factor receptor 1 (FGFR1) Osteogenic progenitor cells Proliferation Osteoblast differentiation |
| title | Nuclear factor I-C regulates intramembranous bone formation via control of FGF signalling |
| title_full | Nuclear factor I-C regulates intramembranous bone formation via control of FGF signalling |
| title_fullStr | Nuclear factor I-C regulates intramembranous bone formation via control of FGF signalling |
| title_full_unstemmed | Nuclear factor I-C regulates intramembranous bone formation via control of FGF signalling |
| title_short | Nuclear factor I-C regulates intramembranous bone formation via control of FGF signalling |
| title_sort | nuclear factor i c regulates intramembranous bone formation via control of fgf signalling |
| topic | Nuclear factor I-C (NFI-C) Fibroblast growth factor receptor 1 (FGFR1) Osteogenic progenitor cells Proliferation Osteoblast differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025001690 |
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