Advances in adipose-derived stem cell harvesting: tissue blocks vs. lipoaspirates
Ensuring a nonenzymatic isolation method for human adipose-derived mesenchymal stem cells (hADSCs) is crucial for safe therapeutic applications. In this study, we introduced an improved mechanical technique to isolate hADSCs from subcutaneous adipose tissue. A total of 382.42 g of adipose tissue was...
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Taibah University for Science |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16583655.2025.2483594 |
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| author | Chun Kai Yew Sadia Farhana Amirul Ashraf Ahmad Shan Wani Siti Fatimah Noor Mat Johar Wan Azman Wan Sulaiman Nur Azida Mohd Nasir |
| author_facet | Chun Kai Yew Sadia Farhana Amirul Ashraf Ahmad Shan Wani Siti Fatimah Noor Mat Johar Wan Azman Wan Sulaiman Nur Azida Mohd Nasir |
| author_sort | Chun Kai Yew |
| collection | DOAJ |
| description | Ensuring a nonenzymatic isolation method for human adipose-derived mesenchymal stem cells (hADSCs) is crucial for safe therapeutic applications. In this study, we introduced an improved mechanical technique to isolate hADSCs from subcutaneous adipose tissue. A total of 382.42 g of adipose tissue was obtained from 19 consented donors, including lipoaspirates (mean: 16.98 g, n = 9) and tissue blocks (mean: 12.11 g, n = 19). Following washing, mincing, and centrifugation, stromal vascular fraction (SVF) cells were collected and subsequently cultured. Notably, the viable SVF yield was 12.47 × 104 cells/ml from tissue blocks and 10.73 × 104 cells/ml from lipoaspirates. Furthermore, at passage 2, hADSCs exhibited typical MSC markers (CD90+/CD73+/CD29+/CD105+/CD34−), while at passage 5, they successfully differentiated into adipocytes, osteoblasts, and chondrocytes. Overall, this method effectively isolates and expands hADSCs without xenogeneic contamination, thereby providing a practical and safe approach for clinical applications. |
| format | Article |
| id | doaj-art-eebd83f0fd5b49bb941854d1ac79ffc6 |
| institution | OA Journals |
| issn | 1658-3655 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Taibah University for Science |
| spelling | doaj-art-eebd83f0fd5b49bb941854d1ac79ffc62025-08-20T01:50:00ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552025-12-0119110.1080/16583655.2025.2483594Advances in adipose-derived stem cell harvesting: tissue blocks vs. lipoaspiratesChun Kai Yew0Sadia Farhana1Amirul Ashraf Ahmad Shan Wani2Siti Fatimah Noor Mat Johar3Wan Azman Wan Sulaiman4Nur Azida Mohd Nasir5Reconstructive Sciences Unit, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, MalaysiaReconstructive Sciences Unit, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, MalaysiaReconstructive Sciences Unit, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, MalaysiaReconstructive Sciences Unit, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, MalaysiaReconstructive Sciences Unit, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, MalaysiaReconstructive Sciences Unit, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, MalaysiaEnsuring a nonenzymatic isolation method for human adipose-derived mesenchymal stem cells (hADSCs) is crucial for safe therapeutic applications. In this study, we introduced an improved mechanical technique to isolate hADSCs from subcutaneous adipose tissue. A total of 382.42 g of adipose tissue was obtained from 19 consented donors, including lipoaspirates (mean: 16.98 g, n = 9) and tissue blocks (mean: 12.11 g, n = 19). Following washing, mincing, and centrifugation, stromal vascular fraction (SVF) cells were collected and subsequently cultured. Notably, the viable SVF yield was 12.47 × 104 cells/ml from tissue blocks and 10.73 × 104 cells/ml from lipoaspirates. Furthermore, at passage 2, hADSCs exhibited typical MSC markers (CD90+/CD73+/CD29+/CD105+/CD34−), while at passage 5, they successfully differentiated into adipocytes, osteoblasts, and chondrocytes. Overall, this method effectively isolates and expands hADSCs without xenogeneic contamination, thereby providing a practical and safe approach for clinical applications.https://www.tandfonline.com/doi/10.1080/16583655.2025.2483594Adipose tissueadipose-derived stem cellsmesenchymal stem cellsadipocyteshumansmechanical isolation |
| spellingShingle | Chun Kai Yew Sadia Farhana Amirul Ashraf Ahmad Shan Wani Siti Fatimah Noor Mat Johar Wan Azman Wan Sulaiman Nur Azida Mohd Nasir Advances in adipose-derived stem cell harvesting: tissue blocks vs. lipoaspirates Journal of Taibah University for Science Adipose tissue adipose-derived stem cells mesenchymal stem cells adipocytes humans mechanical isolation |
| title | Advances in adipose-derived stem cell harvesting: tissue blocks vs. lipoaspirates |
| title_full | Advances in adipose-derived stem cell harvesting: tissue blocks vs. lipoaspirates |
| title_fullStr | Advances in adipose-derived stem cell harvesting: tissue blocks vs. lipoaspirates |
| title_full_unstemmed | Advances in adipose-derived stem cell harvesting: tissue blocks vs. lipoaspirates |
| title_short | Advances in adipose-derived stem cell harvesting: tissue blocks vs. lipoaspirates |
| title_sort | advances in adipose derived stem cell harvesting tissue blocks vs lipoaspirates |
| topic | Adipose tissue adipose-derived stem cells mesenchymal stem cells adipocytes humans mechanical isolation |
| url | https://www.tandfonline.com/doi/10.1080/16583655.2025.2483594 |
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