Genotype Variations and Association between PAI-1 Promoter Region (4G/5G and -844G/A) and Susceptibility to Acute Myocardial Infarction and Chronic Stable Angina
The present study aimed at investigating the 4G/5G and -844G/A polymorphisms and plasma concentration of PAI-1 in patients with acute myocardial infarction (AMI) and chronic stable angina (CSA) in Indian population. It included 100 patients with AMI and stable angina and 100 healthy controls. All st...
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2021-01-01
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| Series: | Cardiology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2021/5551031 |
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| author | Sunil Kumar Amit Kumar Verma Vinay Sagar Ravi Ranjan Rahul Sharma Preeti Tomar Deepti Bhatt Yamini Goyal Mohammed A. Alsahli Ahmad Almatroudi Saleh A. Almatroodi Arshad Husain Rahmani Faris Alrumaihi Khursheed Muzammil Kapil Dev Rakesh Yadav Renu Saxena |
| author_facet | Sunil Kumar Amit Kumar Verma Vinay Sagar Ravi Ranjan Rahul Sharma Preeti Tomar Deepti Bhatt Yamini Goyal Mohammed A. Alsahli Ahmad Almatroudi Saleh A. Almatroodi Arshad Husain Rahmani Faris Alrumaihi Khursheed Muzammil Kapil Dev Rakesh Yadav Renu Saxena |
| author_sort | Sunil Kumar |
| collection | DOAJ |
| description | The present study aimed at investigating the 4G/5G and -844G/A polymorphisms and plasma concentration of PAI-1 in patients with acute myocardial infarction (AMI) and chronic stable angina (CSA) in Indian population. It included 100 patients with AMI and stable angina and 100 healthy controls. All study subjects were typed for two PAI polymorphisms (4G/5G and -844G/A) through PCR-RFLP and level of PAI through ELISA. The comparison of AMI and CSA independently with control in terms of PAI-1 level was statistically significant but not between AMI and CSA. The frequency of 4G/4G and 4G/5G genotype and 4G allele was significantly higher in AMI cases than in control and was found to increase the risk of AMI. There was a significant relationship between 4G/5G polymorphism and AMI risk under the dominant and codominant genotype. The frequency of 4G/4G genotype and 4G allele was significantly higher in CSA cases than in control group and increases the risk of CSA. There was no significant association between 4G/5G polymorphism and CSA risk under recessive, dominant, and codominant models. The genotype and allelic frequencies difference between the cases (AMI and CSA) and control with regard to -844G/A polymorphisms were statistically nonsignificant. Also, we did not detect any significant association of -844G/A polymorphism with AMI and CSA in recessive, dominant, and codominant models. Along with the traditional risk factors, the 4G/5G allele polymorphism is an independent risk factor for the development of AMI. The detection of 4G/5G allele may therefore be helpful in primary prevention. Patients who carry the 4G/5G allele polymorphism have high concentrations of PAI-1, which might be involved in incidents leading to AMI. The present study for the first time revealed significant association of 4G/5G allele polymorphism with high risk of AMI in Indian population and will be helpful in identifying the genetic risk factors associated with AMI and CSA and for better management of diagnostic measures. |
| format | Article |
| id | doaj-art-eeb8093901ce47a5b6171020c4d3d768 |
| institution | Kabale University |
| issn | 2090-8016 2090-0597 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiology Research and Practice |
| spelling | doaj-art-eeb8093901ce47a5b6171020c4d3d7682025-02-03T05:45:20ZengWileyCardiology Research and Practice2090-80162090-05972021-01-01202110.1155/2021/55510315551031Genotype Variations and Association between PAI-1 Promoter Region (4G/5G and -844G/A) and Susceptibility to Acute Myocardial Infarction and Chronic Stable AnginaSunil Kumar0Amit Kumar Verma1Vinay Sagar2Ravi Ranjan3Rahul Sharma4Preeti Tomar5Deepti Bhatt6Yamini Goyal7Mohammed A. Alsahli8Ahmad Almatroudi9Saleh A. Almatroodi10Arshad Husain Rahmani11Faris Alrumaihi12Khursheed Muzammil13Kapil Dev14Rakesh Yadav15Renu Saxena16Department of Microbiology, Government Doon Medical College, Dehradun, IndiaDepartment of Biotechnology, JMI, New Delhi, IndiaDepartment of Internal Medicine, PGIMER, Chandigarh, IndiaDepartment of Hematology, AIIMS, New Delhi, IndiaDepartment of Hematology, AIIMS, New Delhi, IndiaDepartment of Hematology, AIIMS, New Delhi, IndiaDepartment of Biotechnology, JMI, New Delhi, IndiaDepartment of Biotechnology, JMI, New Delhi, IndiaDepartment of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraidah, Saudi ArabiaDepartment of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraidah, Saudi ArabiaDepartment of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraidah, Saudi ArabiaDepartment of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraidah, Saudi ArabiaDepartment of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraidah, Saudi ArabiaDepartment of Public Health, College of Applied Medical Sciences, Khamis Mushayt, King Khalid University, Abha, Saudi ArabiaDepartment of Biotechnology, JMI, New Delhi, IndiaDepartment of Cardiology, AIIMS, New Delhi, IndiaDepartment of Hematology, AIIMS, New Delhi, IndiaThe present study aimed at investigating the 4G/5G and -844G/A polymorphisms and plasma concentration of PAI-1 in patients with acute myocardial infarction (AMI) and chronic stable angina (CSA) in Indian population. It included 100 patients with AMI and stable angina and 100 healthy controls. All study subjects were typed for two PAI polymorphisms (4G/5G and -844G/A) through PCR-RFLP and level of PAI through ELISA. The comparison of AMI and CSA independently with control in terms of PAI-1 level was statistically significant but not between AMI and CSA. The frequency of 4G/4G and 4G/5G genotype and 4G allele was significantly higher in AMI cases than in control and was found to increase the risk of AMI. There was a significant relationship between 4G/5G polymorphism and AMI risk under the dominant and codominant genotype. The frequency of 4G/4G genotype and 4G allele was significantly higher in CSA cases than in control group and increases the risk of CSA. There was no significant association between 4G/5G polymorphism and CSA risk under recessive, dominant, and codominant models. The genotype and allelic frequencies difference between the cases (AMI and CSA) and control with regard to -844G/A polymorphisms were statistically nonsignificant. Also, we did not detect any significant association of -844G/A polymorphism with AMI and CSA in recessive, dominant, and codominant models. Along with the traditional risk factors, the 4G/5G allele polymorphism is an independent risk factor for the development of AMI. The detection of 4G/5G allele may therefore be helpful in primary prevention. Patients who carry the 4G/5G allele polymorphism have high concentrations of PAI-1, which might be involved in incidents leading to AMI. The present study for the first time revealed significant association of 4G/5G allele polymorphism with high risk of AMI in Indian population and will be helpful in identifying the genetic risk factors associated with AMI and CSA and for better management of diagnostic measures.http://dx.doi.org/10.1155/2021/5551031 |
| spellingShingle | Sunil Kumar Amit Kumar Verma Vinay Sagar Ravi Ranjan Rahul Sharma Preeti Tomar Deepti Bhatt Yamini Goyal Mohammed A. Alsahli Ahmad Almatroudi Saleh A. Almatroodi Arshad Husain Rahmani Faris Alrumaihi Khursheed Muzammil Kapil Dev Rakesh Yadav Renu Saxena Genotype Variations and Association between PAI-1 Promoter Region (4G/5G and -844G/A) and Susceptibility to Acute Myocardial Infarction and Chronic Stable Angina Cardiology Research and Practice |
| title | Genotype Variations and Association between PAI-1 Promoter Region (4G/5G and -844G/A) and Susceptibility to Acute Myocardial Infarction and Chronic Stable Angina |
| title_full | Genotype Variations and Association between PAI-1 Promoter Region (4G/5G and -844G/A) and Susceptibility to Acute Myocardial Infarction and Chronic Stable Angina |
| title_fullStr | Genotype Variations and Association between PAI-1 Promoter Region (4G/5G and -844G/A) and Susceptibility to Acute Myocardial Infarction and Chronic Stable Angina |
| title_full_unstemmed | Genotype Variations and Association between PAI-1 Promoter Region (4G/5G and -844G/A) and Susceptibility to Acute Myocardial Infarction and Chronic Stable Angina |
| title_short | Genotype Variations and Association between PAI-1 Promoter Region (4G/5G and -844G/A) and Susceptibility to Acute Myocardial Infarction and Chronic Stable Angina |
| title_sort | genotype variations and association between pai 1 promoter region 4g 5g and 844g a and susceptibility to acute myocardial infarction and chronic stable angina |
| url | http://dx.doi.org/10.1155/2021/5551031 |
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