Characteristics of antioxidant activity for certain fractions of polyphenols from seagrass of genus Zostera
Some components of alcohol extracts from seagrass of genus Zostera were fractioned in the column filled with sorbent Sephadex LH-20 using the methods of size exclusion chromatography. Content of polyphenols in the fractions and their antioxidant activity were determined. The total content of polyphe...
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| Language: | Russian |
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Transactions of the Pacific Research Institute of Fisheries and Oceanography
2021-07-01
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| Series: | Известия ТИНРО |
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| Online Access: | https://izvestiya.tinro-center.ru/jour/article/view/644 |
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| author | N. M. Aminina V. M. Ostapenko E. P. Karaulova |
| author_facet | N. M. Aminina V. M. Ostapenko E. P. Karaulova |
| author_sort | N. M. Aminina |
| collection | DOAJ |
| description | Some components of alcohol extracts from seagrass of genus Zostera were fractioned in the column filled with sorbent Sephadex LH-20 using the methods of size exclusion chromatography. Content of polyphenols in the fractions and their antioxidant activity were determined. The total content of polyphenols in the extracts from Zostera asiatica collected in the Rudnaya Bay (Japan Sea) and Zostera marina from the Aniva Bay (Okhotsk Sea) was 2.10 and 2.15 mg/mL, or 0.4 and 1.8 mg/g DW, respectively. The antioxidant activity varied by fractions in the range 19.1–118.0 μg of ascorbic acid per mL for Z. asiatica and 96.2–213.9 μg of ascorbic acid per mL for Z. marina, in dependence on qualitative and quantitative composition of the fractions. The alcohol extracts of Zostera were heterogeneous systems with different polyphenol components and included protein substances covalently bound with polysaccharides and/or polyphenols, that was confirmed by UV spectrometry and thin-layer chromatography. Phenolic acids, flavones and flavonols were presented in the extracts. |
| format | Article |
| id | doaj-art-eeb78c6d28d64b52b2bdced758bcf057 |
| institution | DOAJ |
| issn | 1606-9919 2658-5510 |
| language | Russian |
| publishDate | 2021-07-01 |
| publisher | Transactions of the Pacific Research Institute of Fisheries and Oceanography |
| record_format | Article |
| series | Известия ТИНРО |
| spelling | doaj-art-eeb78c6d28d64b52b2bdced758bcf0572025-08-20T03:19:00ZrusTransactions of the Pacific Research Institute of Fisheries and OceanographyИзвестия ТИНРО1606-99192658-55102021-07-01201250551510.26428/1606-9919-2021-201-505-515591Characteristics of antioxidant activity for certain fractions of polyphenols from seagrass of genus ZosteraN. M. Aminina0V. M. Ostapenko1E. P. Karaulova2VNIRO (TINRO)VNIRO (TINRO)VNIRO (TINRO)Some components of alcohol extracts from seagrass of genus Zostera were fractioned in the column filled with sorbent Sephadex LH-20 using the methods of size exclusion chromatography. Content of polyphenols in the fractions and their antioxidant activity were determined. The total content of polyphenols in the extracts from Zostera asiatica collected in the Rudnaya Bay (Japan Sea) and Zostera marina from the Aniva Bay (Okhotsk Sea) was 2.10 and 2.15 mg/mL, or 0.4 and 1.8 mg/g DW, respectively. The antioxidant activity varied by fractions in the range 19.1–118.0 μg of ascorbic acid per mL for Z. asiatica and 96.2–213.9 μg of ascorbic acid per mL for Z. marina, in dependence on qualitative and quantitative composition of the fractions. The alcohol extracts of Zostera were heterogeneous systems with different polyphenol components and included protein substances covalently bound with polysaccharides and/or polyphenols, that was confirmed by UV spectrometry and thin-layer chromatography. Phenolic acids, flavones and flavonols were presented in the extracts.https://izvestiya.tinro-center.ru/jour/article/view/644polyphenolantioxidant activityzostera asiaticazostera marinaalcohol extractfractionationthin-layer chromatographyuv spectrometry |
| spellingShingle | N. M. Aminina V. M. Ostapenko E. P. Karaulova Characteristics of antioxidant activity for certain fractions of polyphenols from seagrass of genus Zostera Известия ТИНРО polyphenol antioxidant activity zostera asiatica zostera marina alcohol extract fractionation thin-layer chromatography uv spectrometry |
| title | Characteristics of antioxidant activity for certain fractions of polyphenols from seagrass of genus Zostera |
| title_full | Characteristics of antioxidant activity for certain fractions of polyphenols from seagrass of genus Zostera |
| title_fullStr | Characteristics of antioxidant activity for certain fractions of polyphenols from seagrass of genus Zostera |
| title_full_unstemmed | Characteristics of antioxidant activity for certain fractions of polyphenols from seagrass of genus Zostera |
| title_short | Characteristics of antioxidant activity for certain fractions of polyphenols from seagrass of genus Zostera |
| title_sort | characteristics of antioxidant activity for certain fractions of polyphenols from seagrass of genus zostera |
| topic | polyphenol antioxidant activity zostera asiatica zostera marina alcohol extract fractionation thin-layer chromatography uv spectrometry |
| url | https://izvestiya.tinro-center.ru/jour/article/view/644 |
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