Acute Leukemias: Immunophenotypic Differences between Blast Cells and Their Nonneoplastic Analogues in Bone Marrow
Flow cytometry immunophenotyping of bone marrow tumor blasts is one of the principal methods used for acute leukemia (AL) diagnosing. Normal lymphopoietic and myelopoietic progenitors have very similar antigenic profile with leukemic cells, thus, making the AL diagnosing more difficult. Genetic diso...
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| Format: | Article |
| Language: | Russian |
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Practical Medicine Publishing House
2016-07-01
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| Series: | Клиническая онкогематология |
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| Online Access: | http://bloodjournal.ru/en/acute-leukemias-immunophenotypic-differences-between-blast-cells-and-their-nonneoplastic-analogues-in-bone-marrow/ |
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| _version_ | 1849761577498050560 |
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| author | АM Popov ТYu Verzhbitskaya LG Fechina AV Shestopalov SA Plyasunova |
| author_facet | АM Popov ТYu Verzhbitskaya LG Fechina AV Shestopalov SA Plyasunova |
| author_sort | АM Popov |
| collection | DOAJ |
| description | Flow cytometry immunophenotyping of bone marrow tumor blasts is one of the principal methods used for acute leukemia (AL) diagnosing. Normal lymphopoietic and myelopoietic progenitors have very similar antigenic profile with leukemic cells, thus, making the AL diagnosing more difficult. Genetic disorders resulting in formation of a tumor clone contribute to development of an immunophenotype that differs from normal cells. Aberrant expression of markers detected in AL blast cells alone forms a so-called leukemia-associated immunophenotype. The leukemia-associated immunophenotype detection by multicolor flow cytometry permits distinguishing between normal and neoplastic cells. This requires simultaneous assessment of many markers on the same cells, which is possible only if multicolor flow cytometry with well-designed and well-established antibodies panels is used. Moreover, correct interpretation of the cell population location on dot plot requires adequate cytometer setup, standardized sample preparation and enough experienced personnel. That is why correct immunophenotyping is often possible only in large laboratories performing reference immunophenotyping within the frames of multicenter trials. |
| format | Article |
| id | doaj-art-eeae7c0168874526876294c97d07efb7 |
| institution | DOAJ |
| issn | 1997-6933 2500-2139 |
| language | Russian |
| publishDate | 2016-07-01 |
| publisher | Practical Medicine Publishing House |
| record_format | Article |
| series | Клиническая онкогематология |
| spelling | doaj-art-eeae7c0168874526876294c97d07efb72025-08-20T03:06:00ZrusPractical Medicine Publishing HouseКлиническая онкогематология1997-69332500-21392016-07-019330231310.21320/2500-2139-2016-9-3-302-313Acute Leukemias: Immunophenotypic Differences between Blast Cells and Their Nonneoplastic Analogues in Bone MarrowАM Popov0ТYu Verzhbitskaya1LG Fechina2AV Shestopalov3SA Plyasunova4Dmitrii Rogachev Federal Scientific Clinical Centre of Pediatric Hematology, Oncology and Immunology, 1 Samory Mashela str., Moscow, Russian Federation, 117997Regional Children’s Hospital No. 1, 32 Serafimy Deryabinoi str., Yekaterinburg, Russian Federation, 620149; Institute of Medical Cell Technologies, 22a Karla Marksa str., Yekaterinburg, Russian Federation, 620026Regional Children’s Hospital No. 1, 32 Serafimy Deryabinoi str., Yekaterinburg, Russian Federation, 620149Dmitrii Rogachev Federal Scientific Clinical Centre of Pediatric Hematology, Oncology and Immunology, 1 Samory Mashela str., Moscow, Russian Federation, 117997; N.I. Pirogov Russian National Research Medical University, 1 Samory Mashela str., Moscow, Russian Federation, 117997Dmitrii Rogachev Federal Scientific Clinical Centre of Pediatric Hematology, Oncology and Immunology, 1 Samory Mashela str., Moscow, Russian Federation, 117997Flow cytometry immunophenotyping of bone marrow tumor blasts is one of the principal methods used for acute leukemia (AL) diagnosing. Normal lymphopoietic and myelopoietic progenitors have very similar antigenic profile with leukemic cells, thus, making the AL diagnosing more difficult. Genetic disorders resulting in formation of a tumor clone contribute to development of an immunophenotype that differs from normal cells. Aberrant expression of markers detected in AL blast cells alone forms a so-called leukemia-associated immunophenotype. The leukemia-associated immunophenotype detection by multicolor flow cytometry permits distinguishing between normal and neoplastic cells. This requires simultaneous assessment of many markers on the same cells, which is possible only if multicolor flow cytometry with well-designed and well-established antibodies panels is used. Moreover, correct interpretation of the cell population location on dot plot requires adequate cytometer setup, standardized sample preparation and enough experienced personnel. That is why correct immunophenotyping is often possible only in large laboratories performing reference immunophenotyping within the frames of multicenter trials.http://bloodjournal.ru/en/acute-leukemias-immunophenotypic-differences-between-blast-cells-and-their-nonneoplastic-analogues-in-bone-marrow/acute leukemiasflow cytometryantigenic expressionimmunophenotype |
| spellingShingle | АM Popov ТYu Verzhbitskaya LG Fechina AV Shestopalov SA Plyasunova Acute Leukemias: Immunophenotypic Differences between Blast Cells and Their Nonneoplastic Analogues in Bone Marrow Клиническая онкогематология acute leukemias flow cytometry antigenic expression immunophenotype |
| title | Acute Leukemias: Immunophenotypic Differences between Blast Cells and Their Nonneoplastic Analogues in Bone Marrow |
| title_full | Acute Leukemias: Immunophenotypic Differences between Blast Cells and Their Nonneoplastic Analogues in Bone Marrow |
| title_fullStr | Acute Leukemias: Immunophenotypic Differences between Blast Cells and Their Nonneoplastic Analogues in Bone Marrow |
| title_full_unstemmed | Acute Leukemias: Immunophenotypic Differences between Blast Cells and Their Nonneoplastic Analogues in Bone Marrow |
| title_short | Acute Leukemias: Immunophenotypic Differences between Blast Cells and Their Nonneoplastic Analogues in Bone Marrow |
| title_sort | acute leukemias immunophenotypic differences between blast cells and their nonneoplastic analogues in bone marrow |
| topic | acute leukemias flow cytometry antigenic expression immunophenotype |
| url | http://bloodjournal.ru/en/acute-leukemias-immunophenotypic-differences-between-blast-cells-and-their-nonneoplastic-analogues-in-bone-marrow/ |
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