Autoinducer-2 relieves soil stress-induced dormancy of Bacillus velezensis by modulating sporulation signaling
Abstract The collective behavior of bacteria is regulated by quorum sensing (QS). Autoinducer-2 (AI-2) is a common QS signal that regulates the behavior of both Gram-positive and Gram-negative bacteria. Despite the plethora of processes described to be influenced by AI-2 in diverse Gram-negative bac...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-11-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-024-00594-6 |
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| author | Qin Xiong Huihui Zhang Xia Shu Xiting Sun Haichao Feng Zhihui Xu Ákos T. Kovács Ruifu Zhang Yunpeng Liu |
| author_facet | Qin Xiong Huihui Zhang Xia Shu Xiting Sun Haichao Feng Zhihui Xu Ákos T. Kovács Ruifu Zhang Yunpeng Liu |
| author_sort | Qin Xiong |
| collection | DOAJ |
| description | Abstract The collective behavior of bacteria is regulated by quorum sensing (QS). Autoinducer-2 (AI-2) is a common QS signal that regulates the behavior of both Gram-positive and Gram-negative bacteria. Despite the plethora of processes described to be influenced by AI-2 in diverse Gram-negative bacteria, the AI-2-regulated processes in Bacilli are relatively unexplored. Here, we describe a novel function for AI-2 in Bacillus velezensis SQR9 related to the sporulation. AI-2 inhibited the initiation of sporulation through the phosphatase RapC and the DNA binding regulator ComA. Using biochemistry experiments, we demonstrated that AI-2 interacts with RapC to stimulate its binding to ComA, which leads to an inactive ComA and subsequently a sporulation inhibition. The AI-2 molecule could be shared across species for inhibiting Bacillus sporulation and it also plays the same role in different soil conditions. Our study revealed a novel function and regulatory mechanism of AI-2 in inhibiting sporulation in Bacilli. |
| format | Article |
| id | doaj-art-eeacbbcfd1f74f7f90feed835672d007 |
| institution | OA Journals |
| issn | 2055-5008 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj-art-eeacbbcfd1f74f7f90feed835672d0072025-08-20T02:18:15ZengNature Portfolionpj Biofilms and Microbiomes2055-50082024-11-0110111110.1038/s41522-024-00594-6Autoinducer-2 relieves soil stress-induced dormancy of Bacillus velezensis by modulating sporulation signalingQin Xiong0Huihui Zhang1Xia Shu2Xiting Sun3Haichao Feng4Zhihui Xu5Ákos T. Kovács6Ruifu Zhang7Yunpeng Liu8National Engineering Research Center of Navel Orange, Gannan Normal UniversityJiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural UniversityState Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural SciencesState Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural SciencesJiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural UniversityJiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural UniversityFaculty of Science - Institute of Biology, Leiden University, Sylviusweg 73State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural SciencesState Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural SciencesAbstract The collective behavior of bacteria is regulated by quorum sensing (QS). Autoinducer-2 (AI-2) is a common QS signal that regulates the behavior of both Gram-positive and Gram-negative bacteria. Despite the plethora of processes described to be influenced by AI-2 in diverse Gram-negative bacteria, the AI-2-regulated processes in Bacilli are relatively unexplored. Here, we describe a novel function for AI-2 in Bacillus velezensis SQR9 related to the sporulation. AI-2 inhibited the initiation of sporulation through the phosphatase RapC and the DNA binding regulator ComA. Using biochemistry experiments, we demonstrated that AI-2 interacts with RapC to stimulate its binding to ComA, which leads to an inactive ComA and subsequently a sporulation inhibition. The AI-2 molecule could be shared across species for inhibiting Bacillus sporulation and it also plays the same role in different soil conditions. Our study revealed a novel function and regulatory mechanism of AI-2 in inhibiting sporulation in Bacilli.https://doi.org/10.1038/s41522-024-00594-6 |
| spellingShingle | Qin Xiong Huihui Zhang Xia Shu Xiting Sun Haichao Feng Zhihui Xu Ákos T. Kovács Ruifu Zhang Yunpeng Liu Autoinducer-2 relieves soil stress-induced dormancy of Bacillus velezensis by modulating sporulation signaling npj Biofilms and Microbiomes |
| title | Autoinducer-2 relieves soil stress-induced dormancy of Bacillus velezensis by modulating sporulation signaling |
| title_full | Autoinducer-2 relieves soil stress-induced dormancy of Bacillus velezensis by modulating sporulation signaling |
| title_fullStr | Autoinducer-2 relieves soil stress-induced dormancy of Bacillus velezensis by modulating sporulation signaling |
| title_full_unstemmed | Autoinducer-2 relieves soil stress-induced dormancy of Bacillus velezensis by modulating sporulation signaling |
| title_short | Autoinducer-2 relieves soil stress-induced dormancy of Bacillus velezensis by modulating sporulation signaling |
| title_sort | autoinducer 2 relieves soil stress induced dormancy of bacillus velezensis by modulating sporulation signaling |
| url | https://doi.org/10.1038/s41522-024-00594-6 |
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