Drought Influences the Structure, Diversity, and Functionality of the Fungal Community Inhabiting the Grapevine Xylem and Enhances the Abundance of Phaeomoniella chlamydospora

Grapevine productivity worldwide is increasingly threatened by global warming, potentially exacerbating water scarcity. This study examines drought's effects on grapevine xylem fungal communities, particularly regarding Phaeomoniella chlamydospora, linked to esca and Petri diseases. Investigati...

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Main Authors: C. Leal, R. Bujanda, M. J. Carbone, T. Kiss, A. Eichmeier, D. Gramaje, M. M. Maldonado-González
Format: Article
Language:English
Published: The American Phytopathological Society 2024-11-01
Series:Phytobiomes Journal
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Online Access:https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-02-24-0027-R
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author C. Leal
R. Bujanda
M. J. Carbone
T. Kiss
A. Eichmeier
D. Gramaje
M. M. Maldonado-González
author_facet C. Leal
R. Bujanda
M. J. Carbone
T. Kiss
A. Eichmeier
D. Gramaje
M. M. Maldonado-González
author_sort C. Leal
collection DOAJ
description Grapevine productivity worldwide is increasingly threatened by global warming, potentially exacerbating water scarcity. This study examines drought's effects on grapevine xylem fungal communities, particularly regarding Phaeomoniella chlamydospora, linked to esca and Petri diseases. Investigating 1-year-old grafted grapevines under varied water regimes, including severe, moderate, and no water deficit, the research assessed how drought influences fungal diversity, structure, and interactions within the xylem. Utilizing internal transcribed spacer high-throughput amplicon sequencing and droplet-digital PCR, the study tracked changes in fungal composition and P. chlamydospora prevalence over two growing seasons. The induced water stress not only altered the diversity and composition of the fungal microbiome in the xylem vessels but also affected co-occurrence networks, resulting in less complex networks with fewer correlations between taxa, potentially increasing grapevine vulnerability to various biotic and abiotic stresses. Severe water deficit significantly reduced microbial diversity, leading to a shift in the abundance of pathotrophs such as P. chlamydospora in the xylem. This underscores the interconnectedness between water stress, microbiome dynamics, and plant health. The combination of compromised plant defenses, altered physiological conditions, and shifts in the surrounding microbial community may create conditions conducive to increased P. chlamydospora abundance in the xylem vessels of young vines following water stress.
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spelling doaj-art-eeab9dde5ece433a84c4d2e8a504bf652025-08-20T02:32:54ZengThe American Phytopathological SocietyPhytobiomes Journal2471-29062024-11-018452953910.1094/PBIOMES-02-24-0027-RDrought Influences the Structure, Diversity, and Functionality of the Fungal Community Inhabiting the Grapevine Xylem and Enhances the Abundance of Phaeomoniella chlamydosporaC. Leal0R. Bujanda1M. J. Carbone2T. Kiss3A. Eichmeier4D. Gramaje5M. M. Maldonado-González6Instituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas - Universidad de la Rioja - Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071 Logroño, SpainInstituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas - Universidad de la Rioja - Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071 Logroño, SpainDepartmento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Avenida Garzón 780, Montevideo 12900, UruguayMendel University in Brno, Faculty of Horticulture, Mendeleum - Institute of Genetics, Valticka 334, 69144, Lednice, Czech RepublicMendel University in Brno, Faculty of Horticulture, Mendeleum - Institute of Genetics, Valticka 334, 69144, Lednice, Czech RepublicInstituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas - Universidad de la Rioja - Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071 Logroño, SpainInstituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas - Universidad de la Rioja - Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071 Logroño, SpainGrapevine productivity worldwide is increasingly threatened by global warming, potentially exacerbating water scarcity. This study examines drought's effects on grapevine xylem fungal communities, particularly regarding Phaeomoniella chlamydospora, linked to esca and Petri diseases. Investigating 1-year-old grafted grapevines under varied water regimes, including severe, moderate, and no water deficit, the research assessed how drought influences fungal diversity, structure, and interactions within the xylem. Utilizing internal transcribed spacer high-throughput amplicon sequencing and droplet-digital PCR, the study tracked changes in fungal composition and P. chlamydospora prevalence over two growing seasons. The induced water stress not only altered the diversity and composition of the fungal microbiome in the xylem vessels but also affected co-occurrence networks, resulting in less complex networks with fewer correlations between taxa, potentially increasing grapevine vulnerability to various biotic and abiotic stresses. Severe water deficit significantly reduced microbial diversity, leading to a shift in the abundance of pathotrophs such as P. chlamydospora in the xylem. This underscores the interconnectedness between water stress, microbiome dynamics, and plant health. The combination of compromised plant defenses, altered physiological conditions, and shifts in the surrounding microbial community may create conditions conducive to increased P. chlamydospora abundance in the xylem vessels of young vines following water stress.https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-02-24-0027-Rabiotic stressescaglobal warmingmicrobiome dynamicsPetri diseasewater deficit
spellingShingle C. Leal
R. Bujanda
M. J. Carbone
T. Kiss
A. Eichmeier
D. Gramaje
M. M. Maldonado-González
Drought Influences the Structure, Diversity, and Functionality of the Fungal Community Inhabiting the Grapevine Xylem and Enhances the Abundance of Phaeomoniella chlamydospora
Phytobiomes Journal
abiotic stress
esca
global warming
microbiome dynamics
Petri disease
water deficit
title Drought Influences the Structure, Diversity, and Functionality of the Fungal Community Inhabiting the Grapevine Xylem and Enhances the Abundance of Phaeomoniella chlamydospora
title_full Drought Influences the Structure, Diversity, and Functionality of the Fungal Community Inhabiting the Grapevine Xylem and Enhances the Abundance of Phaeomoniella chlamydospora
title_fullStr Drought Influences the Structure, Diversity, and Functionality of the Fungal Community Inhabiting the Grapevine Xylem and Enhances the Abundance of Phaeomoniella chlamydospora
title_full_unstemmed Drought Influences the Structure, Diversity, and Functionality of the Fungal Community Inhabiting the Grapevine Xylem and Enhances the Abundance of Phaeomoniella chlamydospora
title_short Drought Influences the Structure, Diversity, and Functionality of the Fungal Community Inhabiting the Grapevine Xylem and Enhances the Abundance of Phaeomoniella chlamydospora
title_sort drought influences the structure diversity and functionality of the fungal community inhabiting the grapevine xylem and enhances the abundance of phaeomoniella chlamydospora
topic abiotic stress
esca
global warming
microbiome dynamics
Petri disease
water deficit
url https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-02-24-0027-R
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