Adjusted bacterial cooperation in anammox community to adapt to high ammonium in wastewater treatment plant

Bacterial cooperation is very important for anammox bacteria which perform low-carbon and energy-efficient nitrogen removal, yet its variation to adapt to high NH4+-N concentration in actual wastewater treatment plants (WWTPs) remains unclear. Here, we found wide and varied cross-feedings of anammox...

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Main Authors: Yiming Feng, Lingrui Kong, Ru Zheng, Xiaogang Wu, Jianhang Zhou, Xiaochen Xu, Sitong Liu
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Water Research X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2589914724000483
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author Yiming Feng
Lingrui Kong
Ru Zheng
Xiaogang Wu
Jianhang Zhou
Xiaochen Xu
Sitong Liu
author_facet Yiming Feng
Lingrui Kong
Ru Zheng
Xiaogang Wu
Jianhang Zhou
Xiaochen Xu
Sitong Liu
author_sort Yiming Feng
collection DOAJ
description Bacterial cooperation is very important for anammox bacteria which perform low-carbon and energy-efficient nitrogen removal, yet its variation to adapt to high NH4+-N concentration in actual wastewater treatment plants (WWTPs) remains unclear. Here, we found wide and varied cross-feedings of anammox bacteria and symbiotic bacteria in the two series connected full-scale reactors with different NH4+-N concentrations (297.95 ± 54.84 and 76.03 ± 34.01 mg/L) treating sludge digester liquor. The uptake of vitamin B6 as highly effective antioxidants secreted by the symbiotic bacteria was beneficial for anammox bacteria to resist the high NH4+-N concentration and varied dissolved oxygen (DO). When NH4+-N concentration in influent (1785.46 ± 228.5 mg/L) increased, anammox bacteria tended to reduce the amino acids supply to symbiotic bacteria to save metabolic costs. A total of 26.1% bacterial generalists switched to specialists to increase the stability and functional heterogeneity of the microbial community at high NH4+-N conditions. V/A-type ATPase for anammox bacteria to adapt to the change of NH4+-N was highly important to strive against cellular alkalization caused by free ammonia. This study expands the understanding of the adjusted bacterial cooperation within anammox consortia at high NH4+-N conditions, providing new insights into bacterial adaptation to adverse environments from a sociomicrobiology perspective.
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publisher Elsevier
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spelling doaj-art-eea478a4564d4638b337f3af3fbd28302025-08-20T02:32:48ZengElsevierWater Research X2589-91472024-12-012510025810.1016/j.wroa.2024.100258Adjusted bacterial cooperation in anammox community to adapt to high ammonium in wastewater treatment plantYiming Feng0Lingrui Kong1Ru Zheng2Xiaogang Wu3Jianhang Zhou4Xiaochen Xu5Sitong Liu6College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education of China, Beijing, 100871, ChinaCollege of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education of China, Beijing, 100871, ChinaCollege of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education of China, Beijing, 100871, ChinaCollege of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education of China, Beijing, 100871, ChinaCollege of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education of China, Beijing, 100871, ChinaKey Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environment Sciences and Technology, Dalian University of Technology, Linggong Road 2, Dalian, 116024, ChinaCollege of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education of China, Beijing, 100871, China; Corresponding author at: College of Environmental Sciences and Engineering, Peking University, Yiheyuan Road, No.5, Haidian District, Beijing, 100871, China.Bacterial cooperation is very important for anammox bacteria which perform low-carbon and energy-efficient nitrogen removal, yet its variation to adapt to high NH4+-N concentration in actual wastewater treatment plants (WWTPs) remains unclear. Here, we found wide and varied cross-feedings of anammox bacteria and symbiotic bacteria in the two series connected full-scale reactors with different NH4+-N concentrations (297.95 ± 54.84 and 76.03 ± 34.01 mg/L) treating sludge digester liquor. The uptake of vitamin B6 as highly effective antioxidants secreted by the symbiotic bacteria was beneficial for anammox bacteria to resist the high NH4+-N concentration and varied dissolved oxygen (DO). When NH4+-N concentration in influent (1785.46 ± 228.5 mg/L) increased, anammox bacteria tended to reduce the amino acids supply to symbiotic bacteria to save metabolic costs. A total of 26.1% bacterial generalists switched to specialists to increase the stability and functional heterogeneity of the microbial community at high NH4+-N conditions. V/A-type ATPase for anammox bacteria to adapt to the change of NH4+-N was highly important to strive against cellular alkalization caused by free ammonia. This study expands the understanding of the adjusted bacterial cooperation within anammox consortia at high NH4+-N conditions, providing new insights into bacterial adaptation to adverse environments from a sociomicrobiology perspective.http://www.sciencedirect.com/science/article/pii/S2589914724000483Metabolic cross-feedingAnammox consortiaNH4+-N adaptationMicrobial interactionsBacterial survival strategies
spellingShingle Yiming Feng
Lingrui Kong
Ru Zheng
Xiaogang Wu
Jianhang Zhou
Xiaochen Xu
Sitong Liu
Adjusted bacterial cooperation in anammox community to adapt to high ammonium in wastewater treatment plant
Water Research X
Metabolic cross-feeding
Anammox consortia
NH4+-N adaptation
Microbial interactions
Bacterial survival strategies
title Adjusted bacterial cooperation in anammox community to adapt to high ammonium in wastewater treatment plant
title_full Adjusted bacterial cooperation in anammox community to adapt to high ammonium in wastewater treatment plant
title_fullStr Adjusted bacterial cooperation in anammox community to adapt to high ammonium in wastewater treatment plant
title_full_unstemmed Adjusted bacterial cooperation in anammox community to adapt to high ammonium in wastewater treatment plant
title_short Adjusted bacterial cooperation in anammox community to adapt to high ammonium in wastewater treatment plant
title_sort adjusted bacterial cooperation in anammox community to adapt to high ammonium in wastewater treatment plant
topic Metabolic cross-feeding
Anammox consortia
NH4+-N adaptation
Microbial interactions
Bacterial survival strategies
url http://www.sciencedirect.com/science/article/pii/S2589914724000483
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