16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023
Gene PCR and sequencing using 16S ribosomal RNA (rRNA) can help diagnose challenging bacterial infections. Data on the optimal clinical settings for this type of testing are limited. We performed a retrospective study at Mayo Clinic, Rochester, Minnesota, USA, with typically sterile specimens from...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2025-05-01
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| Series: | Emerging Infectious Diseases |
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| Online Access: | https://wwwnc.cdc.gov/eid/article/31/13/24-1101_article |
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| author | Guyu Li Christopher A. Reis Rebecca M. Kruc Ziyuan Zhang Nicholas T. Streck Elizabeth H. Ristagno Jay Mandrekar Matthew J. Wolf James T. Gaensbauer Robin Patel |
| author_facet | Guyu Li Christopher A. Reis Rebecca M. Kruc Ziyuan Zhang Nicholas T. Streck Elizabeth H. Ristagno Jay Mandrekar Matthew J. Wolf James T. Gaensbauer Robin Patel |
| author_sort | Guyu Li |
| collection | DOAJ |
| description |
Gene PCR and sequencing using 16S ribosomal RNA (rRNA) can help diagnose challenging bacterial infections. Data on the optimal clinical settings for this type of testing are limited. We performed a retrospective study at Mayo Clinic, Rochester, Minnesota, USA, with typically sterile specimens from children that underwent 16S rRNA PCR testing during September 2020–December 2023. Of 162 tests performed on 124 patients, 20% were positive; 58% of positive samples were from culture-negative specimens. Fluid specimens were >3 times as likely to test positive as tissue specimens (odds ratio 3.07 [95% CI 1.32–7.11]; p = 0.007), and pleural fluid demonstrated the highest positivity rate (50%). Of 33 positive results, 4 (12%) specimens qualified for reporting to the state health department for communicable diseases. Those single-laboratory findings demonstrate that the highest positivity rate of 16S rRNA PCR and sequencing is pleural fluid, although many specimen types tested positive.
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| format | Article |
| id | doaj-art-ee9d2a2e97254a45a101039fe3bb849c |
| institution | OA Journals |
| issn | 1080-6040 1080-6059 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj-art-ee9d2a2e97254a45a101039fe3bb849c2025-08-20T01:50:18ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592025-05-01311312913610.3201/eid3113.24110116S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023Guyu LiChristopher A. ReisRebecca M. KrucZiyuan ZhangNicholas T. StreckElizabeth H. RistagnoJay MandrekarMatthew J. WolfJames T. GaensbauerRobin Patel Gene PCR and sequencing using 16S ribosomal RNA (rRNA) can help diagnose challenging bacterial infections. Data on the optimal clinical settings for this type of testing are limited. We performed a retrospective study at Mayo Clinic, Rochester, Minnesota, USA, with typically sterile specimens from children that underwent 16S rRNA PCR testing during September 2020–December 2023. Of 162 tests performed on 124 patients, 20% were positive; 58% of positive samples were from culture-negative specimens. Fluid specimens were >3 times as likely to test positive as tissue specimens (odds ratio 3.07 [95% CI 1.32–7.11]; p = 0.007), and pleural fluid demonstrated the highest positivity rate (50%). Of 33 positive results, 4 (12%) specimens qualified for reporting to the state health department for communicable diseases. Those single-laboratory findings demonstrate that the highest positivity rate of 16S rRNA PCR and sequencing is pleural fluid, although many specimen types tested positive. https://wwwnc.cdc.gov/eid/article/31/13/24-1101_article16S ribosomal RNAPCRsequencingbacteriabacterial infectionspediatrics |
| spellingShingle | Guyu Li Christopher A. Reis Rebecca M. Kruc Ziyuan Zhang Nicholas T. Streck Elizabeth H. Ristagno Jay Mandrekar Matthew J. Wolf James T. Gaensbauer Robin Patel 16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023 Emerging Infectious Diseases 16S ribosomal RNA PCR sequencing bacteria bacterial infections pediatrics |
| title | 16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023 |
| title_full | 16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023 |
| title_fullStr | 16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023 |
| title_full_unstemmed | 16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023 |
| title_short | 16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020–2023 |
| title_sort | 16s ribosomal rna gene pcr and sequencing for pediatric infection diagnosis united states 2020 2023 |
| topic | 16S ribosomal RNA PCR sequencing bacteria bacterial infections pediatrics |
| url | https://wwwnc.cdc.gov/eid/article/31/13/24-1101_article |
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