Effects of Transplanted Heparin-Poloxamer Hydrogel Combining Dental Pulp Stem Cells and bFGF on Spinal Cord Injury Repair
Spinal cord injury (SCI) is one of serious traumatic diseases of the central nervous system and has no effective treatment because of its complicated pathophysiology. Tissue engineering strategy which contains scaffolds, cells, and growth factors can provide a promising treatment for SCI. Hydrogel t...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/2398521 |
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| author | Lihua Luo Abdullkhaleg Ali Albashari Xiaoyan Wang Ling Jin Yanni Zhang Lina Zheng Jianjian Xia Helin Xu Yingzheng Zhao Jian Xiao Yan He Qingsong Ye |
| author_facet | Lihua Luo Abdullkhaleg Ali Albashari Xiaoyan Wang Ling Jin Yanni Zhang Lina Zheng Jianjian Xia Helin Xu Yingzheng Zhao Jian Xiao Yan He Qingsong Ye |
| author_sort | Lihua Luo |
| collection | DOAJ |
| description | Spinal cord injury (SCI) is one of serious traumatic diseases of the central nervous system and has no effective treatment because of its complicated pathophysiology. Tissue engineering strategy which contains scaffolds, cells, and growth factors can provide a promising treatment for SCI. Hydrogel that has 3D network structure and biomimetic microenvironment can support cellular growth and embed biological macromolecules for sustaining release. Dental pulp stem cells (DPSCs), derived from cranial neural crest, possess mesenchymal stem cell (MSC) characteristics and have an ability to provide neuroprotective and neurotrophic properties for SCI treatment. Basic fibroblast growth factor (bFGF) is able to promote cell survival and proliferation and also has beneficial effect on neural regeneration and functional recovery after SCI. Herein, a thermosensitive heparin-poloxamer (HP) hydrogel containing DPSCs and bFGF was prepared, and the effects of HP-bFGF-DPSCs on neuron restoration after SCI were evaluated by functional recovery tests, western blotting, magnetic resonance imaging (MRI), histology evaluation, and immunohistochemistry. The results suggested that transplanted HP hydrogel containing DPSCs and bFGF had a significant impact on spinal cord repair and regeneration and may provide a promising strategy for neuron repair, functional recovery, and tissue regeneration after SCI. |
| format | Article |
| id | doaj-art-ee9c988bdef8429db46a6a170f114e27 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-ee9c988bdef8429db46a6a170f114e272025-08-20T02:21:41ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/23985212398521Effects of Transplanted Heparin-Poloxamer Hydrogel Combining Dental Pulp Stem Cells and bFGF on Spinal Cord Injury RepairLihua Luo0Abdullkhaleg Ali Albashari1Xiaoyan Wang2Ling Jin3Yanni Zhang4Lina Zheng5Jianjian Xia6Helin Xu7Yingzheng Zhao8Jian Xiao9Yan He10Qingsong Ye11WMU-UQ Research Group for Regenerative Medicine, Institute of Stem Cells and Tissue Engineering, School of Stomatology, Wenzhou Medical University, Wenzhou 325035, ChinaWMU-UQ Research Group for Regenerative Medicine, Institute of Stem Cells and Tissue Engineering, School of Stomatology, Wenzhou Medical University, Wenzhou 325035, ChinaWMU-UQ Research Group for Regenerative Medicine, Institute of Stem Cells and Tissue Engineering, School of Stomatology, Wenzhou Medical University, Wenzhou 325035, ChinaWMU-UQ Research Group for Regenerative Medicine, Institute of Stem Cells and Tissue Engineering, School of Stomatology, Wenzhou Medical University, Wenzhou 325035, ChinaWMU-UQ Research Group for Regenerative Medicine, Institute of Stem Cells and Tissue Engineering, School of Stomatology, Wenzhou Medical University, Wenzhou 325035, ChinaWMU-UQ Research Group for Regenerative Medicine, Institute of Stem Cells and Tissue Engineering, School of Stomatology, Wenzhou Medical University, Wenzhou 325035, ChinaWMU-UQ Research Group for Regenerative Medicine, Institute of Stem Cells and Tissue Engineering, School of Stomatology, Wenzhou Medical University, Wenzhou 325035, ChinaSchool of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, ChinaSchool of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, ChinaSchool of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, ChinaWMU-UQ Research Group for Regenerative Medicine, Institute of Stem Cells and Tissue Engineering, School of Stomatology, Wenzhou Medical University, Wenzhou 325035, ChinaWMU-UQ Research Group for Regenerative Medicine, Institute of Stem Cells and Tissue Engineering, School of Stomatology, Wenzhou Medical University, Wenzhou 325035, ChinaSpinal cord injury (SCI) is one of serious traumatic diseases of the central nervous system and has no effective treatment because of its complicated pathophysiology. Tissue engineering strategy which contains scaffolds, cells, and growth factors can provide a promising treatment for SCI. Hydrogel that has 3D network structure and biomimetic microenvironment can support cellular growth and embed biological macromolecules for sustaining release. Dental pulp stem cells (DPSCs), derived from cranial neural crest, possess mesenchymal stem cell (MSC) characteristics and have an ability to provide neuroprotective and neurotrophic properties for SCI treatment. Basic fibroblast growth factor (bFGF) is able to promote cell survival and proliferation and also has beneficial effect on neural regeneration and functional recovery after SCI. Herein, a thermosensitive heparin-poloxamer (HP) hydrogel containing DPSCs and bFGF was prepared, and the effects of HP-bFGF-DPSCs on neuron restoration after SCI were evaluated by functional recovery tests, western blotting, magnetic resonance imaging (MRI), histology evaluation, and immunohistochemistry. The results suggested that transplanted HP hydrogel containing DPSCs and bFGF had a significant impact on spinal cord repair and regeneration and may provide a promising strategy for neuron repair, functional recovery, and tissue regeneration after SCI.http://dx.doi.org/10.1155/2018/2398521 |
| spellingShingle | Lihua Luo Abdullkhaleg Ali Albashari Xiaoyan Wang Ling Jin Yanni Zhang Lina Zheng Jianjian Xia Helin Xu Yingzheng Zhao Jian Xiao Yan He Qingsong Ye Effects of Transplanted Heparin-Poloxamer Hydrogel Combining Dental Pulp Stem Cells and bFGF on Spinal Cord Injury Repair Stem Cells International |
| title | Effects of Transplanted Heparin-Poloxamer Hydrogel Combining Dental Pulp Stem Cells and bFGF on Spinal Cord Injury Repair |
| title_full | Effects of Transplanted Heparin-Poloxamer Hydrogel Combining Dental Pulp Stem Cells and bFGF on Spinal Cord Injury Repair |
| title_fullStr | Effects of Transplanted Heparin-Poloxamer Hydrogel Combining Dental Pulp Stem Cells and bFGF on Spinal Cord Injury Repair |
| title_full_unstemmed | Effects of Transplanted Heparin-Poloxamer Hydrogel Combining Dental Pulp Stem Cells and bFGF on Spinal Cord Injury Repair |
| title_short | Effects of Transplanted Heparin-Poloxamer Hydrogel Combining Dental Pulp Stem Cells and bFGF on Spinal Cord Injury Repair |
| title_sort | effects of transplanted heparin poloxamer hydrogel combining dental pulp stem cells and bfgf on spinal cord injury repair |
| url | http://dx.doi.org/10.1155/2018/2398521 |
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