Validation of Polymorphisms Associated with the Immune Response After Vaccination Against Porcine Reproductive and Respiratory Syndrome Virus in Yorkshire Gilts
Porcine respiratory and reproductive syndrome is a viral disease that impacts the health and profitability of swine farms, largely due to significant variation in the vaccination response. The objective was to identify and validate molecular markers associated with the antibody response in gilts fol...
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2025-03-01
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| author | Salvador Icedo-Nuñez Rosa I. Luna-Ramirez R. Mark Enns Scott E. Speidel Jesús Hernández Xi Zeng Miguel A. Sánchez-Castro Carlos M. Aguilar-Trejo Guillermo Luna-Nevárez Martha C. López-González Javier R. Reyna-Granados Pablo Luna-Nevárez |
| author_facet | Salvador Icedo-Nuñez Rosa I. Luna-Ramirez R. Mark Enns Scott E. Speidel Jesús Hernández Xi Zeng Miguel A. Sánchez-Castro Carlos M. Aguilar-Trejo Guillermo Luna-Nevárez Martha C. López-González Javier R. Reyna-Granados Pablo Luna-Nevárez |
| author_sort | Salvador Icedo-Nuñez |
| collection | DOAJ |
| description | Porcine respiratory and reproductive syndrome is a viral disease that impacts the health and profitability of swine farms, largely due to significant variation in the vaccination response. The objective was to identify and validate molecular markers associated with the antibody response in gilts following vaccination against the PRRSV. The study included one hundred (<i>n</i> = 100) 6-month-old Yorkshire gilts that were negative for the PRRSV. Gilts were randomly assigned to one of two treatments, PRRS-vaccinated (<i>n</i> = 75) and control (<i>n</i> = 25) groups. Blood samples collected on day 21 were analyzed to evaluate the antibody response, as indicated by the sample-to-positive (S/P) ratio, to the PRRSV following vaccination. DNA was extracted and genotyped using a low-density chip containing 10,000 single nucleotide polymorphisms (SNPs). A genome-wide association study (GWAS) was conducted to identify candidate SNPs associated with the S/P ratio, which were validated in two independent gilt populations (<i>n</i> = 226). The SNPs rs707264998, rs708860811, and rs81358818 in the genes <i>RNF144B</i>, <i>XKR9</i>, and <i>BMAL1</i>, respectively, were significantly associated (<i>p</i> < 0.01) with the S/P ratio and demonstrated an additive effect. In conclusion, three SNPs are proposed as candidate markers for an enhanced immune response to vaccination against the PRRSV and may be beneficial in genetic selection programs. |
| format | Article |
| id | doaj-art-ee9a22a2db4946e5bf4963bf3828f5ba |
| institution | OA Journals |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-ee9a22a2db4946e5bf4963bf3828f5ba2025-08-20T02:25:08ZengMDPI AGVeterinary Sciences2306-73812025-03-0112429510.3390/vetsci12040295Validation of Polymorphisms Associated with the Immune Response After Vaccination Against Porcine Reproductive and Respiratory Syndrome Virus in Yorkshire GiltsSalvador Icedo-Nuñez0Rosa I. Luna-Ramirez1R. Mark Enns2Scott E. Speidel3Jesús Hernández4Xi Zeng5Miguel A. Sánchez-Castro6Carlos M. Aguilar-Trejo7Guillermo Luna-Nevárez8Martha C. López-González9Javier R. Reyna-Granados10Pablo Luna-Nevárez11Departamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, Ciudad Obregón 85000, MexicoSchool of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ 85721, USADepartment of Animal Sciences, Colorado State University, Fort Collins, CO 80523, USADepartment of Animal Sciences, Colorado State University, Fort Collins, CO 80523, USALaboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo A.C., Hermosillo 83304, MexicoZoetis Inc., VMRD Genetics R&D, Kalamazoo, MI 49007, USAZoetis Inc., VMRD Genetics R&D, Kalamazoo, MI 49007, USADepartamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, Ciudad Obregón 85000, MexicoDepartamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, Ciudad Obregón 85000, MexicoDepartamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, Ciudad Obregón 85000, MexicoDepartamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, Ciudad Obregón 85000, MexicoDepartamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, Ciudad Obregón 85000, MexicoPorcine respiratory and reproductive syndrome is a viral disease that impacts the health and profitability of swine farms, largely due to significant variation in the vaccination response. The objective was to identify and validate molecular markers associated with the antibody response in gilts following vaccination against the PRRSV. The study included one hundred (<i>n</i> = 100) 6-month-old Yorkshire gilts that were negative for the PRRSV. Gilts were randomly assigned to one of two treatments, PRRS-vaccinated (<i>n</i> = 75) and control (<i>n</i> = 25) groups. Blood samples collected on day 21 were analyzed to evaluate the antibody response, as indicated by the sample-to-positive (S/P) ratio, to the PRRSV following vaccination. DNA was extracted and genotyped using a low-density chip containing 10,000 single nucleotide polymorphisms (SNPs). A genome-wide association study (GWAS) was conducted to identify candidate SNPs associated with the S/P ratio, which were validated in two independent gilt populations (<i>n</i> = 226). The SNPs rs707264998, rs708860811, and rs81358818 in the genes <i>RNF144B</i>, <i>XKR9</i>, and <i>BMAL1</i>, respectively, were significantly associated (<i>p</i> < 0.01) with the S/P ratio and demonstrated an additive effect. In conclusion, three SNPs are proposed as candidate markers for an enhanced immune response to vaccination against the PRRSV and may be beneficial in genetic selection programs.https://www.mdpi.com/2306-7381/12/4/295antibody responseDNAgenetic markerspolymorphismPRRS |
| spellingShingle | Salvador Icedo-Nuñez Rosa I. Luna-Ramirez R. Mark Enns Scott E. Speidel Jesús Hernández Xi Zeng Miguel A. Sánchez-Castro Carlos M. Aguilar-Trejo Guillermo Luna-Nevárez Martha C. López-González Javier R. Reyna-Granados Pablo Luna-Nevárez Validation of Polymorphisms Associated with the Immune Response After Vaccination Against Porcine Reproductive and Respiratory Syndrome Virus in Yorkshire Gilts Veterinary Sciences antibody response DNA genetic markers polymorphism PRRS |
| title | Validation of Polymorphisms Associated with the Immune Response After Vaccination Against Porcine Reproductive and Respiratory Syndrome Virus in Yorkshire Gilts |
| title_full | Validation of Polymorphisms Associated with the Immune Response After Vaccination Against Porcine Reproductive and Respiratory Syndrome Virus in Yorkshire Gilts |
| title_fullStr | Validation of Polymorphisms Associated with the Immune Response After Vaccination Against Porcine Reproductive and Respiratory Syndrome Virus in Yorkshire Gilts |
| title_full_unstemmed | Validation of Polymorphisms Associated with the Immune Response After Vaccination Against Porcine Reproductive and Respiratory Syndrome Virus in Yorkshire Gilts |
| title_short | Validation of Polymorphisms Associated with the Immune Response After Vaccination Against Porcine Reproductive and Respiratory Syndrome Virus in Yorkshire Gilts |
| title_sort | validation of polymorphisms associated with the immune response after vaccination against porcine reproductive and respiratory syndrome virus in yorkshire gilts |
| topic | antibody response DNA genetic markers polymorphism PRRS |
| url | https://www.mdpi.com/2306-7381/12/4/295 |
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