Chemical Composition and Biological Activities of <i>Lagopsis supina</i> Extract: Antioxidant, Adipogenic, and Ani-Inflammatory Effects
Background/Objectives: <i>Lagopsis supina</i>, a traditional Chinese medicine valued for its diuretic properties, has limited research on its antioxidant, adipogenic, and anti-inflammatory effects. This study aimed to investigate the chemical composition and biological activities of <...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/18/2/150 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849719716157849600 |
|---|---|
| author | Juhyun Choi Duc Dat Le Nayoung Roh Jiseok Lee Deumaya Shrestha Thientam Dinh Vinhquang Truong Badamtsetseg Bazarragchaa Soo-Yong Kim Sung-Suk Suh Mina Lee Jong Bae Seo |
| author_facet | Juhyun Choi Duc Dat Le Nayoung Roh Jiseok Lee Deumaya Shrestha Thientam Dinh Vinhquang Truong Badamtsetseg Bazarragchaa Soo-Yong Kim Sung-Suk Suh Mina Lee Jong Bae Seo |
| author_sort | Juhyun Choi |
| collection | DOAJ |
| description | Background/Objectives: <i>Lagopsis supina</i>, a traditional Chinese medicine valued for its diuretic properties, has limited research on its antioxidant, adipogenic, and anti-inflammatory effects. This study aimed to investigate the chemical composition and biological activities of <i>Lagopsis supina</i> extract (LSE). Methods: LSE was prepared and evaluated for antioxidant activity, effects on adipocyte differentiation in 3T3-L1 preadipocytes, and anti-inflammatory properties in RAW 264.7 macrophages. Ultra-high-performance liquid chromatography-electrospray ionization Orbitrap tandem mass spectrometry (UHPLC-ESI-Orbitrap-MS/MS)-based molecular networking was used to characterize its secondary metabolites. Results: LSE exhibited antioxidant activity in DPPH and ABTS assays. It significantly enhanced the differentiation of 3T3-L1 preadipocytes into mature adipocytes during early and intermediate stages by upregulating adipogenic transcription factors such as PPARγ, C/EBPα, and C/EBPβ, along with promoting cyclin E expression. LSE also increased PPARγ activity and the expression of its target genes, such as Glut 4, PEPCK, FABP4, and Plin2. Moreover, LSE inhibited lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophages by downregulating pro-inflammatory mediators (iNOS, COX-2, TNF-α, IL-6) and inhibiting extracellular signal-regulated kinase (ERK) phosphorylation. Chemical profiling revealed eight major compound groups: glycosides, organic acids, terpenoids, flavonoids, phenylglycosides, phenolics, fatty acids, and others characterized by their mass fragmentation patterns, precursors, and UV absorption spectra. In silico analysis confirmed these compounds’ bioactivities, demonstrating strong interactions and binding affinities with antioxidant, adipogenic, and anti-inflammatory protein targets. Conclusions: These findings highlight LSE’s triple therapeutic potential: antioxidant activity, adipogenesis promotion, and inflammation attenuation. LSE emerges as a promising therapeutic candidate for managing obesity and related inflammatory complications. |
| format | Article |
| id | doaj-art-ee8a6dddad8744338541286c5424b72d |
| institution | DOAJ |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-ee8a6dddad8744338541286c5424b72d2025-08-20T03:12:05ZengMDPI AGPharmaceuticals1424-82472025-01-0118215010.3390/ph18020150Chemical Composition and Biological Activities of <i>Lagopsis supina</i> Extract: Antioxidant, Adipogenic, and Ani-Inflammatory EffectsJuhyun Choi0Duc Dat Le1Nayoung Roh2Jiseok Lee3Deumaya Shrestha4Thientam Dinh5Vinhquang Truong6Badamtsetseg Bazarragchaa7Soo-Yong Kim8Sung-Suk Suh9Mina Lee10Jong Bae Seo11Department of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Muan 58554, Jeonnam, Republic of KoreaCollege of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, 255 Jungangno, Suncheon 57922, Jeonnam, Republic of KoreaDepartment of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Muan 58554, Jeonnam, Republic of KoreaDepartment of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Muan 58554, Jeonnam, Republic of KoreaDepartment of Biosciences, Mokpo National University, Muan 58554, Jeonnam, Republic of KoreaCollege of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, 255 Jungangno, Suncheon 57922, Jeonnam, Republic of KoreaCollege of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, 255 Jungangno, Suncheon 57922, Jeonnam, Republic of KoreaNatural History Museum of Mongolia, Ulaanbaatar 15141, MongoliaInternational Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of KoreaDepartment of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Muan 58554, Jeonnam, Republic of KoreaCollege of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, 255 Jungangno, Suncheon 57922, Jeonnam, Republic of KoreaDepartment of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Muan 58554, Jeonnam, Republic of KoreaBackground/Objectives: <i>Lagopsis supina</i>, a traditional Chinese medicine valued for its diuretic properties, has limited research on its antioxidant, adipogenic, and anti-inflammatory effects. This study aimed to investigate the chemical composition and biological activities of <i>Lagopsis supina</i> extract (LSE). Methods: LSE was prepared and evaluated for antioxidant activity, effects on adipocyte differentiation in 3T3-L1 preadipocytes, and anti-inflammatory properties in RAW 264.7 macrophages. Ultra-high-performance liquid chromatography-electrospray ionization Orbitrap tandem mass spectrometry (UHPLC-ESI-Orbitrap-MS/MS)-based molecular networking was used to characterize its secondary metabolites. Results: LSE exhibited antioxidant activity in DPPH and ABTS assays. It significantly enhanced the differentiation of 3T3-L1 preadipocytes into mature adipocytes during early and intermediate stages by upregulating adipogenic transcription factors such as PPARγ, C/EBPα, and C/EBPβ, along with promoting cyclin E expression. LSE also increased PPARγ activity and the expression of its target genes, such as Glut 4, PEPCK, FABP4, and Plin2. Moreover, LSE inhibited lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophages by downregulating pro-inflammatory mediators (iNOS, COX-2, TNF-α, IL-6) and inhibiting extracellular signal-regulated kinase (ERK) phosphorylation. Chemical profiling revealed eight major compound groups: glycosides, organic acids, terpenoids, flavonoids, phenylglycosides, phenolics, fatty acids, and others characterized by their mass fragmentation patterns, precursors, and UV absorption spectra. In silico analysis confirmed these compounds’ bioactivities, demonstrating strong interactions and binding affinities with antioxidant, adipogenic, and anti-inflammatory protein targets. Conclusions: These findings highlight LSE’s triple therapeutic potential: antioxidant activity, adipogenesis promotion, and inflammation attenuation. LSE emerges as a promising therapeutic candidate for managing obesity and related inflammatory complications.https://www.mdpi.com/1424-8247/18/2/150<i>Lagopsis supina</i>antioxidantadipogenic transcriptionUHPLC-MS/MSinflammation |
| spellingShingle | Juhyun Choi Duc Dat Le Nayoung Roh Jiseok Lee Deumaya Shrestha Thientam Dinh Vinhquang Truong Badamtsetseg Bazarragchaa Soo-Yong Kim Sung-Suk Suh Mina Lee Jong Bae Seo Chemical Composition and Biological Activities of <i>Lagopsis supina</i> Extract: Antioxidant, Adipogenic, and Ani-Inflammatory Effects Pharmaceuticals <i>Lagopsis supina</i> antioxidant adipogenic transcription UHPLC-MS/MS inflammation |
| title | Chemical Composition and Biological Activities of <i>Lagopsis supina</i> Extract: Antioxidant, Adipogenic, and Ani-Inflammatory Effects |
| title_full | Chemical Composition and Biological Activities of <i>Lagopsis supina</i> Extract: Antioxidant, Adipogenic, and Ani-Inflammatory Effects |
| title_fullStr | Chemical Composition and Biological Activities of <i>Lagopsis supina</i> Extract: Antioxidant, Adipogenic, and Ani-Inflammatory Effects |
| title_full_unstemmed | Chemical Composition and Biological Activities of <i>Lagopsis supina</i> Extract: Antioxidant, Adipogenic, and Ani-Inflammatory Effects |
| title_short | Chemical Composition and Biological Activities of <i>Lagopsis supina</i> Extract: Antioxidant, Adipogenic, and Ani-Inflammatory Effects |
| title_sort | chemical composition and biological activities of i lagopsis supina i extract antioxidant adipogenic and ani inflammatory effects |
| topic | <i>Lagopsis supina</i> antioxidant adipogenic transcription UHPLC-MS/MS inflammation |
| url | https://www.mdpi.com/1424-8247/18/2/150 |
| work_keys_str_mv | AT juhyunchoi chemicalcompositionandbiologicalactivitiesofilagopsissupinaiextractantioxidantadipogenicandaniinflammatoryeffects AT ducdatle chemicalcompositionandbiologicalactivitiesofilagopsissupinaiextractantioxidantadipogenicandaniinflammatoryeffects AT nayoungroh chemicalcompositionandbiologicalactivitiesofilagopsissupinaiextractantioxidantadipogenicandaniinflammatoryeffects AT jiseoklee chemicalcompositionandbiologicalactivitiesofilagopsissupinaiextractantioxidantadipogenicandaniinflammatoryeffects AT deumayashrestha chemicalcompositionandbiologicalactivitiesofilagopsissupinaiextractantioxidantadipogenicandaniinflammatoryeffects AT thientamdinh chemicalcompositionandbiologicalactivitiesofilagopsissupinaiextractantioxidantadipogenicandaniinflammatoryeffects AT vinhquangtruong chemicalcompositionandbiologicalactivitiesofilagopsissupinaiextractantioxidantadipogenicandaniinflammatoryeffects AT badamtsetsegbazarragchaa chemicalcompositionandbiologicalactivitiesofilagopsissupinaiextractantioxidantadipogenicandaniinflammatoryeffects AT sooyongkim chemicalcompositionandbiologicalactivitiesofilagopsissupinaiextractantioxidantadipogenicandaniinflammatoryeffects AT sungsuksuh chemicalcompositionandbiologicalactivitiesofilagopsissupinaiextractantioxidantadipogenicandaniinflammatoryeffects AT minalee chemicalcompositionandbiologicalactivitiesofilagopsissupinaiextractantioxidantadipogenicandaniinflammatoryeffects AT jongbaeseo chemicalcompositionandbiologicalactivitiesofilagopsissupinaiextractantioxidantadipogenicandaniinflammatoryeffects |