Urinary Excretion of Kidney Aquaporins as Possible Diagnostic Biomarker of Diabetic Nephropathy
Diabetic nephropathy (DN) is a microangiopathic complication of diabetes mellitus (DM) affecting one-third of diabetic patients. The large variability in the clinical presentation of renal involvement in patients with DM makes kidney biopsy a prerequisite for a correct diagnosis. However, renal biop...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/4360357 |
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| author | Luigi Rossi Maria Celeste Nicoletti Monica Carmosino Lisa Mastrofrancesco Antonella Di Franco Francesca Indrio Rossella Lella Luigi Laviola Francesco Giorgino Maria Svelto Loreto Gesualdo Giuseppe Procino |
| author_facet | Luigi Rossi Maria Celeste Nicoletti Monica Carmosino Lisa Mastrofrancesco Antonella Di Franco Francesca Indrio Rossella Lella Luigi Laviola Francesco Giorgino Maria Svelto Loreto Gesualdo Giuseppe Procino |
| author_sort | Luigi Rossi |
| collection | DOAJ |
| description | Diabetic nephropathy (DN) is a microangiopathic complication of diabetes mellitus (DM) affecting one-third of diabetic patients. The large variability in the clinical presentation of renal involvement in patients with DM makes kidney biopsy a prerequisite for a correct diagnosis. However, renal biopsy is an invasive procedure associated with risk of major complications. Numerous studies aimed to identify a noninvasive biomarker of DN but, so far, none of these is considered to be sufficiently specific and sensitive. Water channel aquaporins (AQPs), expressed at the plasma membrane of epithelial tubular cells, are often dysregulated during DN. In this work, we analyzed the urine excretion of AQP5 and AQP2 (uAQP5 and uAQP2), via exosomes, in 35 diabetic patients: 12 normoalbuminuric with normal renal function (DM), 11 with proteinuric nondiabetic nephropathy (NDN), and 12 with histological diagnosis and classification of DN. ELISA and WB analysis independently showed that uAQP5 was significantly increased in DN patients. Interestingly, linear regression analysis showed a positive correlation between uAQP5 and the histological class of DN. The same analysis, focusing on uAQP2, showed comparable results. Taken together, these data suggest a possible use of AQP5 and AQP2 as novel noninvasive biomarkers to help in classifying the clinical stage of DN. |
| format | Article |
| id | doaj-art-ee88a8ece9ab49418cf85dd911e6ec31 |
| institution | DOAJ |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-ee88a8ece9ab49418cf85dd911e6ec312025-08-20T03:20:43ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/43603574360357Urinary Excretion of Kidney Aquaporins as Possible Diagnostic Biomarker of Diabetic NephropathyLuigi Rossi0Maria Celeste Nicoletti1Monica Carmosino2Lisa Mastrofrancesco3Antonella Di Franco4Francesca Indrio5Rossella Lella6Luigi Laviola7Francesco Giorgino8Maria Svelto9Loreto Gesualdo10Giuseppe Procino11DETO, University of Bari, Bari, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Bari, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Bari, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Bari, ItalyDETO, University of Bari, Bari, ItalyDETO, University of Bari, Bari, ItalyDETO, University of Bari, Bari, ItalyDETO, University of Bari, Bari, ItalyDETO, University of Bari, Bari, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Bari, ItalyDETO, University of Bari, Bari, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Bari, ItalyDiabetic nephropathy (DN) is a microangiopathic complication of diabetes mellitus (DM) affecting one-third of diabetic patients. The large variability in the clinical presentation of renal involvement in patients with DM makes kidney biopsy a prerequisite for a correct diagnosis. However, renal biopsy is an invasive procedure associated with risk of major complications. Numerous studies aimed to identify a noninvasive biomarker of DN but, so far, none of these is considered to be sufficiently specific and sensitive. Water channel aquaporins (AQPs), expressed at the plasma membrane of epithelial tubular cells, are often dysregulated during DN. In this work, we analyzed the urine excretion of AQP5 and AQP2 (uAQP5 and uAQP2), via exosomes, in 35 diabetic patients: 12 normoalbuminuric with normal renal function (DM), 11 with proteinuric nondiabetic nephropathy (NDN), and 12 with histological diagnosis and classification of DN. ELISA and WB analysis independently showed that uAQP5 was significantly increased in DN patients. Interestingly, linear regression analysis showed a positive correlation between uAQP5 and the histological class of DN. The same analysis, focusing on uAQP2, showed comparable results. Taken together, these data suggest a possible use of AQP5 and AQP2 as novel noninvasive biomarkers to help in classifying the clinical stage of DN.http://dx.doi.org/10.1155/2017/4360357 |
| spellingShingle | Luigi Rossi Maria Celeste Nicoletti Monica Carmosino Lisa Mastrofrancesco Antonella Di Franco Francesca Indrio Rossella Lella Luigi Laviola Francesco Giorgino Maria Svelto Loreto Gesualdo Giuseppe Procino Urinary Excretion of Kidney Aquaporins as Possible Diagnostic Biomarker of Diabetic Nephropathy Journal of Diabetes Research |
| title | Urinary Excretion of Kidney Aquaporins as Possible Diagnostic Biomarker of Diabetic Nephropathy |
| title_full | Urinary Excretion of Kidney Aquaporins as Possible Diagnostic Biomarker of Diabetic Nephropathy |
| title_fullStr | Urinary Excretion of Kidney Aquaporins as Possible Diagnostic Biomarker of Diabetic Nephropathy |
| title_full_unstemmed | Urinary Excretion of Kidney Aquaporins as Possible Diagnostic Biomarker of Diabetic Nephropathy |
| title_short | Urinary Excretion of Kidney Aquaporins as Possible Diagnostic Biomarker of Diabetic Nephropathy |
| title_sort | urinary excretion of kidney aquaporins as possible diagnostic biomarker of diabetic nephropathy |
| url | http://dx.doi.org/10.1155/2017/4360357 |
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