A Label-Free Aptasensor for the Detection of Sulfaquinoxaline Using AuNPs and Aptamer in Water Environment
Sulfaquinoxaline (SQX) is widely utilized in aquaculture and animal husbandry due to its broad antimicrobial spectrum and low cost. However, it is difficult to degrade, and there are relevant residues in the aquatic environment, which could be harmful to both the ecological environment and human hea...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/15/1/30 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588943643639808 |
|---|---|
| author | Zhaoyang Zhou Xingyue Chen Shuang Jiang Zhuoer Chen Sixian Wang Yueyang Ren Xiaodong Fan Tao Le |
| author_facet | Zhaoyang Zhou Xingyue Chen Shuang Jiang Zhuoer Chen Sixian Wang Yueyang Ren Xiaodong Fan Tao Le |
| author_sort | Zhaoyang Zhou |
| collection | DOAJ |
| description | Sulfaquinoxaline (SQX) is widely utilized in aquaculture and animal husbandry due to its broad antimicrobial spectrum and low cost. However, it is difficult to degrade, and there are relevant residues in the aquatic environment, which could be harmful to both the ecological environment and human health. As a new recognition molecule, the aptamer can be recognized with SQX with high affinity and specificity, and the aptamer is no longer adsorbed to AuNPs after binding to SQX, which weakens the catalytic effect of AuNPs. Consequently, an aptasensor for the detection of SQX was successfully developed. This aptasensor exhibits a linear range of 40–640 ng/mL, with a detection limit of 36.95 ng/mL, demonstrating both sensitivity and selectivity. The recoveries of this aptasensor in water samples ranged from 90 to 109.9%, which was quite in line with high-performance liquid chromatography. These findings suggest that the aptasensor is a valuable tool for detecting SQX in aqueous environmental samples. |
| format | Article |
| id | doaj-art-ee812080cc2d4e80b28dbce5b6955217 |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-ee812080cc2d4e80b28dbce5b69552172025-01-24T13:25:29ZengMDPI AGBiosensors2079-63742025-01-011513010.3390/bios15010030A Label-Free Aptasensor for the Detection of Sulfaquinoxaline Using AuNPs and Aptamer in Water EnvironmentZhaoyang Zhou0Xingyue Chen1Shuang Jiang2Zhuoer Chen3Sixian Wang4Yueyang Ren5Xiaodong Fan6Tao Le7Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, ChinaChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, ChinaChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, ChinaChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, ChinaChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, ChinaChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, ChinaChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, ChinaChongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, ChinaSulfaquinoxaline (SQX) is widely utilized in aquaculture and animal husbandry due to its broad antimicrobial spectrum and low cost. However, it is difficult to degrade, and there are relevant residues in the aquatic environment, which could be harmful to both the ecological environment and human health. As a new recognition molecule, the aptamer can be recognized with SQX with high affinity and specificity, and the aptamer is no longer adsorbed to AuNPs after binding to SQX, which weakens the catalytic effect of AuNPs. Consequently, an aptasensor for the detection of SQX was successfully developed. This aptasensor exhibits a linear range of 40–640 ng/mL, with a detection limit of 36.95 ng/mL, demonstrating both sensitivity and selectivity. The recoveries of this aptasensor in water samples ranged from 90 to 109.9%, which was quite in line with high-performance liquid chromatography. These findings suggest that the aptasensor is a valuable tool for detecting SQX in aqueous environmental samples.https://www.mdpi.com/2079-6374/15/1/30sulfaquinoxalineAuNPsaptasensorresidueenvironment |
| spellingShingle | Zhaoyang Zhou Xingyue Chen Shuang Jiang Zhuoer Chen Sixian Wang Yueyang Ren Xiaodong Fan Tao Le A Label-Free Aptasensor for the Detection of Sulfaquinoxaline Using AuNPs and Aptamer in Water Environment Biosensors sulfaquinoxaline AuNPs aptasensor residue environment |
| title | A Label-Free Aptasensor for the Detection of Sulfaquinoxaline Using AuNPs and Aptamer in Water Environment |
| title_full | A Label-Free Aptasensor for the Detection of Sulfaquinoxaline Using AuNPs and Aptamer in Water Environment |
| title_fullStr | A Label-Free Aptasensor for the Detection of Sulfaquinoxaline Using AuNPs and Aptamer in Water Environment |
| title_full_unstemmed | A Label-Free Aptasensor for the Detection of Sulfaquinoxaline Using AuNPs and Aptamer in Water Environment |
| title_short | A Label-Free Aptasensor for the Detection of Sulfaquinoxaline Using AuNPs and Aptamer in Water Environment |
| title_sort | label free aptasensor for the detection of sulfaquinoxaline using aunps and aptamer in water environment |
| topic | sulfaquinoxaline AuNPs aptasensor residue environment |
| url | https://www.mdpi.com/2079-6374/15/1/30 |
| work_keys_str_mv | AT zhaoyangzhou alabelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT xingyuechen alabelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT shuangjiang alabelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT zhuoerchen alabelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT sixianwang alabelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT yueyangren alabelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT xiaodongfan alabelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT taole alabelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT zhaoyangzhou labelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT xingyuechen labelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT shuangjiang labelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT zhuoerchen labelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT sixianwang labelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT yueyangren labelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT xiaodongfan labelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment AT taole labelfreeaptasensorforthedetectionofsulfaquinoxalineusingaunpsandaptamerinwaterenvironment |