Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction

Solid-phase reversible immobilization (SPRI) beads are widely used for high-throughput sequencing library construction to purify and recover nucleic acids. This research was aimed at investigating the effects of SPRI bead ratio, incubation time, and elution time on nucleic acid recovery during full-...

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Bibliographic Details
Main Authors: Yinmei Li, Ziqiang He, Mimi Kong, Dong Jin
Format: Article
Language:English
Published: Compuscript Ltd 2023-09-01
Series:Zoonoses
Online Access:https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2023-0007
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