Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae)
The low stringency-polymerase chain reaction (LS-PCR) and loop-mediated isothermal amplification (LAMP) assays were used to detect the presence of S. mansoni DNA in (1) Brazilian intermediate hosts (Biomphalaria glabrata, B. straminea, and B. tenagophila) with patent S. mansoni infections, (2) B. gl...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2017/8628971 |
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| author | Roberta Lima Caldeira Liana Konovaloffi Jannotti-Passos Omar Dos Santos Carvalho |
| author_facet | Roberta Lima Caldeira Liana Konovaloffi Jannotti-Passos Omar Dos Santos Carvalho |
| author_sort | Roberta Lima Caldeira |
| collection | DOAJ |
| description | The low stringency-polymerase chain reaction (LS-PCR) and loop-mediated isothermal amplification (LAMP) assays were used to detect the presence of S. mansoni DNA in (1) Brazilian intermediate hosts (Biomphalaria glabrata, B. straminea, and B. tenagophila) with patent S. mansoni infections, (2) B. glabrata snails with prepatent S. mansoni infections, (3) various mixtures of infected and noninfected snails; and (4) snails infected with other trematode species. The assays showed high sensitivity and specificity and could detect S. mansoni DNA when one positive snail was included in a pool of 1,000 negative specimens of Biomphalaria. These molecular approaches can provide a low-cost, effective, and rapid method for detecting the presence of S. mansoni in pooled samples of field-collected Biomphalaria. These assays should aid mapping of transmission sites in endemic areas, especially in low prevalence regions and improve schistosomiasis surveillance. It will be a useful tool to monitor low infection rates of snails in areas where control interventions are leading towards the elimination of schistosomiasis. |
| format | Article |
| id | doaj-art-ee7c78ccc3a54765ae77dfac4d8e348e |
| institution | Kabale University |
| issn | 1687-9686 1687-9694 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-ee7c78ccc3a54765ae77dfac4d8e348e2025-02-03T01:23:46ZengWileyJournal of Tropical Medicine1687-96861687-96942017-01-01201710.1155/2017/86289718628971Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae)Roberta Lima Caldeira0Liana Konovaloffi Jannotti-Passos1Omar Dos Santos Carvalho2Laboratório de Helmintologia e Malacologia Médica, Centro de Pesquisas René Rachou/Fiocruz, Av. Augusto de Lima, 1715 Belo Horizonte, MG, BrazilMoluscário Lobato Paraense, Centro de Pesquisas René Rachou/Fiocruz, Av. Augusto de Lima, 1715 Belo Horizonte, MG, BrazilLaboratório de Helmintologia e Malacologia Médica, Centro de Pesquisas René Rachou/Fiocruz, Av. Augusto de Lima, 1715 Belo Horizonte, MG, BrazilThe low stringency-polymerase chain reaction (LS-PCR) and loop-mediated isothermal amplification (LAMP) assays were used to detect the presence of S. mansoni DNA in (1) Brazilian intermediate hosts (Biomphalaria glabrata, B. straminea, and B. tenagophila) with patent S. mansoni infections, (2) B. glabrata snails with prepatent S. mansoni infections, (3) various mixtures of infected and noninfected snails; and (4) snails infected with other trematode species. The assays showed high sensitivity and specificity and could detect S. mansoni DNA when one positive snail was included in a pool of 1,000 negative specimens of Biomphalaria. These molecular approaches can provide a low-cost, effective, and rapid method for detecting the presence of S. mansoni in pooled samples of field-collected Biomphalaria. These assays should aid mapping of transmission sites in endemic areas, especially in low prevalence regions and improve schistosomiasis surveillance. It will be a useful tool to monitor low infection rates of snails in areas where control interventions are leading towards the elimination of schistosomiasis.http://dx.doi.org/10.1155/2017/8628971 |
| spellingShingle | Roberta Lima Caldeira Liana Konovaloffi Jannotti-Passos Omar Dos Santos Carvalho Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae) Journal of Tropical Medicine |
| title | Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae) |
| title_full | Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae) |
| title_fullStr | Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae) |
| title_full_unstemmed | Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae) |
| title_short | Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae) |
| title_sort | use of molecular methods for the rapid mass detection of schistosoma mansoni platyhelminthes trematoda in biomphalaria spp gastropoda planorbidae |
| url | http://dx.doi.org/10.1155/2017/8628971 |
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