circRNA-79530 regulates Twist-mediated mitochondrial damage via sponging miR-214 affecting hypoxia/reoxygenation-induced injury in H9c2 cardiomyocytes
Cardiomyocyte injury related to hypoxia/reoxygenation (H/R) is pivotal in myocardial infarction. The circular RNA circRNA-79530 (circ79530) may play a regulatory role in this process, though its exact function has yet to be elucidated. This research explores the role of circRNA-79530 in H9c2 cells...
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PAGEPress Publications
2025-08-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | https://www.ejh.it/ejh/article/view/4230 |
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| author | Ziyang Yu Wenbo Xu Yirong Teng Tingting Li Ren Guo Ju Li Xichen Li Yanping Li Yinglu Hao |
| author_facet | Ziyang Yu Wenbo Xu Yirong Teng Tingting Li Ren Guo Ju Li Xichen Li Yanping Li Yinglu Hao |
| author_sort | Ziyang Yu |
| collection | DOAJ |
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Cardiomyocyte injury related to hypoxia/reoxygenation (H/R) is pivotal in myocardial infarction. The circular RNA circRNA-79530 (circ79530) may play a regulatory role in this process, though its exact function has yet to be elucidated. This research explores the role of circRNA-79530 in H9c2 cells under H/R, with a particular focus on its interactions with miR-214 and the transcription factor Twist. It also examines their subsequent effects on mitochondrial function and oxidative stress. H9c2 cardiomyocytes were subjected to H/R to model myocardial injury. We measured circRNA-79530, miR-214, and Twist levels via RT-qPCR, with Twist protein via Western blotting. ROS levels were quantified using DCFH-DA, and cell viability and injuries were assessed through CCK-8, LDH, SOD, and MDA assays, respectively. Mitochondrial performance was assessed through various methods, including the measurement of mitochondrial membrane potential using JC-1 staining, the quantification of ATP levels, and the examination of the protein levels of mitochondrial complexes, as well as the expression of fusion proteins. Our findings indicated that downregulation of circRNA-79530 modulated miR-214 and Twist expression, influencing mitochondrial dynamics and ROS production. Knockdown of circRNA-79530 improved cell viability, reduced oxidative stress and enhanced mitochondrial function. Additionally, overexpression of miR-214 mitigated Twist expression, further supporting the effect of miR-214 in H/R conditions. circRNA-79530 could worsen oxidative stress and mitochondrial dysfunction, and regulate Twist-mediated mitochondrial damage via sponging miR-214 in H9c2 cells under H/R conditions.
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| format | Article |
| id | doaj-art-ee7b23db083d491b9e6073271d3ee1a2 |
| institution | Kabale University |
| issn | 1121-760X 2038-8306 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | PAGEPress Publications |
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| series | European Journal of Histochemistry |
| spelling | doaj-art-ee7b23db083d491b9e6073271d3ee1a22025-08-23T11:18:21ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062025-08-0169310.4081/ejh.2025.4230circRNA-79530 regulates Twist-mediated mitochondrial damage via sponging miR-214 affecting hypoxia/reoxygenation-induced injury in H9c2 cardiomyocytesZiyang Yu0Wenbo Xu1Yirong Teng2Tingting Li3Ren Guo4Ju Li5Xichen Li6Yanping Li7Yinglu Hao8Department of Cardiology, The 6th Affiliated Hospital of Kunming Medical University, The People's Hospital of Yuxi CityDepartment of Laboratory, The 6th Affiliated Hospital of Kunming Medical University, The People's Hospital of Yuxi CityDepartment of General Practice, The 6th Affiliated Hospital of Kunming Medical University, The People's Hospital of Yuxi CityDepartment of Cardiology, The 6th Affiliated Hospital of Kunming Medical University, The People's Hospital of Yuxi CityDepartment of Cardiology, The 6th Affiliated Hospital of Kunming Medical University, The People's Hospital of Yuxi CityDepartment of Cardiology, The 6th Affiliated Hospital of Kunming Medical University, The People's Hospital of Yuxi CityDepartment of Cardiology, The 6th Affiliated Hospital of Kunming Medical University, The People's Hospital of Yuxi CityDepartment of Cardiology, The 6th Affiliated Hospital of Kunming Medical University, The People's Hospital of Yuxi CityDepartment of Cardiology, The 6th Affiliated Hospital of Kunming Medical University, The People's Hospital of Yuxi City Cardiomyocyte injury related to hypoxia/reoxygenation (H/R) is pivotal in myocardial infarction. The circular RNA circRNA-79530 (circ79530) may play a regulatory role in this process, though its exact function has yet to be elucidated. This research explores the role of circRNA-79530 in H9c2 cells under H/R, with a particular focus on its interactions with miR-214 and the transcription factor Twist. It also examines their subsequent effects on mitochondrial function and oxidative stress. H9c2 cardiomyocytes were subjected to H/R to model myocardial injury. We measured circRNA-79530, miR-214, and Twist levels via RT-qPCR, with Twist protein via Western blotting. ROS levels were quantified using DCFH-DA, and cell viability and injuries were assessed through CCK-8, LDH, SOD, and MDA assays, respectively. Mitochondrial performance was assessed through various methods, including the measurement of mitochondrial membrane potential using JC-1 staining, the quantification of ATP levels, and the examination of the protein levels of mitochondrial complexes, as well as the expression of fusion proteins. Our findings indicated that downregulation of circRNA-79530 modulated miR-214 and Twist expression, influencing mitochondrial dynamics and ROS production. Knockdown of circRNA-79530 improved cell viability, reduced oxidative stress and enhanced mitochondrial function. Additionally, overexpression of miR-214 mitigated Twist expression, further supporting the effect of miR-214 in H/R conditions. circRNA-79530 could worsen oxidative stress and mitochondrial dysfunction, and regulate Twist-mediated mitochondrial damage via sponging miR-214 in H9c2 cells under H/R conditions. https://www.ejh.it/ejh/article/view/4230Cardiomyocyte injuryhypoxia/reoxygenationcircRNA-79530 |
| spellingShingle | Ziyang Yu Wenbo Xu Yirong Teng Tingting Li Ren Guo Ju Li Xichen Li Yanping Li Yinglu Hao circRNA-79530 regulates Twist-mediated mitochondrial damage via sponging miR-214 affecting hypoxia/reoxygenation-induced injury in H9c2 cardiomyocytes European Journal of Histochemistry Cardiomyocyte injury hypoxia/reoxygenation circRNA-79530 |
| title | circRNA-79530 regulates Twist-mediated mitochondrial damage via sponging miR-214 affecting hypoxia/reoxygenation-induced injury in H9c2 cardiomyocytes |
| title_full | circRNA-79530 regulates Twist-mediated mitochondrial damage via sponging miR-214 affecting hypoxia/reoxygenation-induced injury in H9c2 cardiomyocytes |
| title_fullStr | circRNA-79530 regulates Twist-mediated mitochondrial damage via sponging miR-214 affecting hypoxia/reoxygenation-induced injury in H9c2 cardiomyocytes |
| title_full_unstemmed | circRNA-79530 regulates Twist-mediated mitochondrial damage via sponging miR-214 affecting hypoxia/reoxygenation-induced injury in H9c2 cardiomyocytes |
| title_short | circRNA-79530 regulates Twist-mediated mitochondrial damage via sponging miR-214 affecting hypoxia/reoxygenation-induced injury in H9c2 cardiomyocytes |
| title_sort | circrna 79530 regulates twist mediated mitochondrial damage via sponging mir 214 affecting hypoxia reoxygenation induced injury in h9c2 cardiomyocytes |
| topic | Cardiomyocyte injury hypoxia/reoxygenation circRNA-79530 |
| url | https://www.ejh.it/ejh/article/view/4230 |
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