Effectiveness of ice bath therapy after high-intensity exercise in reducing inflammation, oxidative stress, and creatine kinase activity in adolescent males

Introduction: Recovery is a vital component of physical activity, serving to restore the body’s physiological equilibrium after intense exertion. However, effective and efficient models of recovery therapy remain underreported in current literature. Objective: This quasi-experimental study aimed...

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Main Authors: Adi Pranoto, Irmantara Subagio, Gigih Siantoro, Afif Rusdiawan, Mochamad Purnomo, Donny Ardy Kusuma, Bhekti Lestari, Muhammad Kharis Fajar, I Dewa Made Aryananda Wijaya Kusuma, Bekir Erhan Orhan
Format: Article
Language:English
Published: FEADEF 2025-06-01
Series:Retos: Nuevas Tendencias en Educación Física, Deportes y Recreación
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Online Access:https://revistaretos.org/index.php/retos/article/view/116129
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author Adi Pranoto
Irmantara Subagio
Gigih Siantoro
Afif Rusdiawan
Mochamad Purnomo
Donny Ardy Kusuma
Bhekti Lestari
Muhammad Kharis Fajar
I Dewa Made Aryananda Wijaya Kusuma
Bekir Erhan Orhan
author_facet Adi Pranoto
Irmantara Subagio
Gigih Siantoro
Afif Rusdiawan
Mochamad Purnomo
Donny Ardy Kusuma
Bhekti Lestari
Muhammad Kharis Fajar
I Dewa Made Aryananda Wijaya Kusuma
Bekir Erhan Orhan
author_sort Adi Pranoto
collection DOAJ
description Introduction: Recovery is a vital component of physical activity, serving to restore the body’s physiological equilibrium after intense exertion. However, effective and efficient models of recovery therapy remain underreported in current literature. Objective: This quasi-experimental study aimed to examine the effects of ice bath therapy following high-intensity exercise on markers of inflammation, oxidative stress, and creatine kinase (CK) activity in adolescent males. Methodology: Twenty male undergraduate students (n = 20; aged 19–22 years) majoring in Football Coaching at the Faculty of Sports and Health Sciences, Universitas Negeri Surabaya (UNESA), participated in this study. They received ice bath therapy (IBT) after high-intensity exercise for four weeks. IL-6 concentrations were measured using an ELISA Kit, MDA levels with a Colorimetric Assay Kit, and CK activity with an Activity Assay Kit. Paired and independent sample t-tests were used, and Cohen’s d was calculated to determine effect size at a 95% confidence level. Results: Significant reductions were observed in Interleukin-6 (IL-6) by 29.49 %, Malondialdehyde (MDA) by 37.75 %, and CK activity by 84.45 % after intervention in the IBT group (all p ≤ 0.05). Between-group comparisons also revealed significant differences in all three biomarkers (all p ≤ 0.05), with large to very large effect sizes (Cohen’s d > 1.7), indicating a strong impact of the therapy. Conclusions: Ice bath therapy after high-intensity exercise can be an effective therapy model in significantly reducing levels of IL-6, MDA, and CK activity in adolescent males.
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spelling doaj-art-ee7a6ef2465a4166afe798804671379c2025-08-20T03:20:55ZengFEADEFRetos: Nuevas Tendencias en Educación Física, Deportes y Recreación1579-17261988-20412025-06-016810.47197/retos.v68.116129Effectiveness of ice bath therapy after high-intensity exercise in reducing inflammation, oxidative stress, and creatine kinase activity in adolescent malesAdi Pranoto0https://orcid.org/0000-0003-4080-9245Irmantara Subagio1Gigih Siantoro2Afif Rusdiawan3Mochamad Purnomo4Donny Ardy Kusuma5Bhekti Lestari6Muhammad Kharis Fajar7I Dewa Made Aryananda Wijaya Kusuma8Bekir Erhan Orhan9Department of Sports Coaching Education, Faculty of Sports and Health Science, Universitas Negeri SurabayaDepartment of Sport Coaching Education, Faculty of Sport and Health Sciences, Universitas Negeri SurabayaDepartment of Sport Coaching Education, Faculty of Sport and Health Sciences, Universitas Negeri SurabayaDepartment of Sport Science, Faculty of Sport and Health Sciences, Universitas Negeri SurabayaSport and Exercise Research Center, Universitas Negeri SurabayaSport and Exercise Research Center, Universitas Negeri SurabayaDepartment of Sport Coaching Education, Faculty of Sport and Health Sciences, Universitas Negeri SurabayaDepartemen of Sport Coaching, Faculty of Vocational, Universitas Negeri SurabayaDepartment of Sport Coaching Education, Faculty of Sport and Health Sciences, Universitas Negeri SurabayaFaculty of Sports Sciences, Istanbul Aydın University Introduction: Recovery is a vital component of physical activity, serving to restore the body’s physiological equilibrium after intense exertion. However, effective and efficient models of recovery therapy remain underreported in current literature. Objective: This quasi-experimental study aimed to examine the effects of ice bath therapy following high-intensity exercise on markers of inflammation, oxidative stress, and creatine kinase (CK) activity in adolescent males. Methodology: Twenty male undergraduate students (n = 20; aged 19–22 years) majoring in Football Coaching at the Faculty of Sports and Health Sciences, Universitas Negeri Surabaya (UNESA), participated in this study. They received ice bath therapy (IBT) after high-intensity exercise for four weeks. IL-6 concentrations were measured using an ELISA Kit, MDA levels with a Colorimetric Assay Kit, and CK activity with an Activity Assay Kit. Paired and independent sample t-tests were used, and Cohen’s d was calculated to determine effect size at a 95% confidence level. Results: Significant reductions were observed in Interleukin-6 (IL-6) by 29.49 %, Malondialdehyde (MDA) by 37.75 %, and CK activity by 84.45 % after intervention in the IBT group (all p ≤ 0.05). Between-group comparisons also revealed significant differences in all three biomarkers (all p ≤ 0.05), with large to very large effect sizes (Cohen’s d > 1.7), indicating a strong impact of the therapy. Conclusions: Ice bath therapy after high-intensity exercise can be an effective therapy model in significantly reducing levels of IL-6, MDA, and CK activity in adolescent males. https://revistaretos.org/index.php/retos/article/view/116129High-intensity exerciseice bath therapyrecovery modesoccer athlete
spellingShingle Adi Pranoto
Irmantara Subagio
Gigih Siantoro
Afif Rusdiawan
Mochamad Purnomo
Donny Ardy Kusuma
Bhekti Lestari
Muhammad Kharis Fajar
I Dewa Made Aryananda Wijaya Kusuma
Bekir Erhan Orhan
Effectiveness of ice bath therapy after high-intensity exercise in reducing inflammation, oxidative stress, and creatine kinase activity in adolescent males
Retos: Nuevas Tendencias en Educación Física, Deportes y Recreación
High-intensity exercise
ice bath therapy
recovery mode
soccer athlete
title Effectiveness of ice bath therapy after high-intensity exercise in reducing inflammation, oxidative stress, and creatine kinase activity in adolescent males
title_full Effectiveness of ice bath therapy after high-intensity exercise in reducing inflammation, oxidative stress, and creatine kinase activity in adolescent males
title_fullStr Effectiveness of ice bath therapy after high-intensity exercise in reducing inflammation, oxidative stress, and creatine kinase activity in adolescent males
title_full_unstemmed Effectiveness of ice bath therapy after high-intensity exercise in reducing inflammation, oxidative stress, and creatine kinase activity in adolescent males
title_short Effectiveness of ice bath therapy after high-intensity exercise in reducing inflammation, oxidative stress, and creatine kinase activity in adolescent males
title_sort effectiveness of ice bath therapy after high intensity exercise in reducing inflammation oxidative stress and creatine kinase activity in adolescent males
topic High-intensity exercise
ice bath therapy
recovery mode
soccer athlete
url https://revistaretos.org/index.php/retos/article/view/116129
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