GhWRKY75 positively regulates GhPR6-5b via binding to a W-box TTGAC (C/T) to orchestrate cotton resistance to Verticillium dahliae
Verticillium dahliae is an important fungal pathogen affecting cotton yield and quality. Therefore, the mining of V. dahlia-resistance genes is urgently needed. Proteases and protease inhibitors play crucial roles in plant defense responses. However, the functions and regulatory mechanisms of the pr...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
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KeAi Communications Co., Ltd.
2024-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311924002089 |
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| author | Qichao Chai Meina Zheng Yanli Li Mingwei Gao Yongcui Wang Xiuli Wang Chao Zhang Hui Jiang Ying Chen Jiabao Wang Junsheng Zhao |
| author_facet | Qichao Chai Meina Zheng Yanli Li Mingwei Gao Yongcui Wang Xiuli Wang Chao Zhang Hui Jiang Ying Chen Jiabao Wang Junsheng Zhao |
| author_sort | Qichao Chai |
| collection | DOAJ |
| description | Verticillium dahliae is an important fungal pathogen affecting cotton yield and quality. Therefore, the mining of V. dahlia-resistance genes is urgently needed. Proteases and protease inhibitors play crucial roles in plant defense responses. However, the functions and regulatory mechanisms of the protease inhibitor PR6 gene family remain largely unknown. This study provides a comprehensive analysis of the PR6 gene family in the cotton genome. We performed genome-wide identification and functional characterization of the cotton GhPR6 gene family, which belongs to the potato protease inhibitor I family of inhibitors. Thirty-nine PR6s were identified in Gossypium arboreum, G. raimondii, G. barbadense, and G. hirsutum, and they were clustered into four groups. Based on the analysis of pathogen-induced and Ghlmm transcriptome data, GhPR6-5b was identified as the key gene for V. dahliae resistance. Virus-induced gene silencing experiments revealed that cotton was more sensitive to V. dahliae V991 after PR6-5b silencing. The present study established that GhWRKY75 plays an important role in resistance to Verticillium wilt in cotton by positively regulating GhPR6-5b expression by directly binding to the W-box TTGAC(T/C). Our findings established that GhWRKY75 is a potential candidate for improving cotton resistance to V. dahliae, and provide primary information for further investigations and the development of specific strategies to bolster the defense mechanisms of cotton against V. dahliae. |
| format | Article |
| id | doaj-art-ee692c274961418c8b30407b6cd814c8 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-ee692c274961418c8b30407b6cd814c82025-08-20T03:57:55ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192024-10-0123103343335710.1016/j.jia.2024.05.017GhWRKY75 positively regulates GhPR6-5b via binding to a W-box TTGAC (C/T) to orchestrate cotton resistance to Verticillium dahliaeQichao Chai0Meina Zheng1Yanli Li2Mingwei Gao3Yongcui Wang4Xiuli Wang5Chao Zhang6Hui Jiang7Ying Chen8Jiabao Wang9Junsheng Zhao10Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China, Tel: +86-531-66659004Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China, Tel: +86-531-66659004; College of Life Sciences, Shandong Normal University, Jinan 250014, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China, Tel: +86-531-66659004Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China, Tel: +86-531-66659004Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China, Tel: +86-531-66659004Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China, Tel: +86-531-66659004Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China, Tel: +86-531-66659004Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China, Tel: +86-531-66659004Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China, Tel: +86-531-66659004Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China, Tel: +86-531-66659004Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China, Tel: +86-531-66659004; College of Life Sciences, Shandong Normal University, Jinan 250014, China; Correspondence Junsheng Zhao, Tel: +86-531-66658256Verticillium dahliae is an important fungal pathogen affecting cotton yield and quality. Therefore, the mining of V. dahlia-resistance genes is urgently needed. Proteases and protease inhibitors play crucial roles in plant defense responses. However, the functions and regulatory mechanisms of the protease inhibitor PR6 gene family remain largely unknown. This study provides a comprehensive analysis of the PR6 gene family in the cotton genome. We performed genome-wide identification and functional characterization of the cotton GhPR6 gene family, which belongs to the potato protease inhibitor I family of inhibitors. Thirty-nine PR6s were identified in Gossypium arboreum, G. raimondii, G. barbadense, and G. hirsutum, and they were clustered into four groups. Based on the analysis of pathogen-induced and Ghlmm transcriptome data, GhPR6-5b was identified as the key gene for V. dahliae resistance. Virus-induced gene silencing experiments revealed that cotton was more sensitive to V. dahliae V991 after PR6-5b silencing. The present study established that GhWRKY75 plays an important role in resistance to Verticillium wilt in cotton by positively regulating GhPR6-5b expression by directly binding to the W-box TTGAC(T/C). Our findings established that GhWRKY75 is a potential candidate for improving cotton resistance to V. dahliae, and provide primary information for further investigations and the development of specific strategies to bolster the defense mechanisms of cotton against V. dahliae.http://www.sciencedirect.com/science/article/pii/S2095311924002089cottonproteinase inhibitorsWRKY transcription factorVerticillium wilt |
| spellingShingle | Qichao Chai Meina Zheng Yanli Li Mingwei Gao Yongcui Wang Xiuli Wang Chao Zhang Hui Jiang Ying Chen Jiabao Wang Junsheng Zhao GhWRKY75 positively regulates GhPR6-5b via binding to a W-box TTGAC (C/T) to orchestrate cotton resistance to Verticillium dahliae Journal of Integrative Agriculture cotton proteinase inhibitors WRKY transcription factor Verticillium wilt |
| title | GhWRKY75 positively regulates GhPR6-5b via binding to a W-box TTGAC (C/T) to orchestrate cotton resistance to Verticillium dahliae |
| title_full | GhWRKY75 positively regulates GhPR6-5b via binding to a W-box TTGAC (C/T) to orchestrate cotton resistance to Verticillium dahliae |
| title_fullStr | GhWRKY75 positively regulates GhPR6-5b via binding to a W-box TTGAC (C/T) to orchestrate cotton resistance to Verticillium dahliae |
| title_full_unstemmed | GhWRKY75 positively regulates GhPR6-5b via binding to a W-box TTGAC (C/T) to orchestrate cotton resistance to Verticillium dahliae |
| title_short | GhWRKY75 positively regulates GhPR6-5b via binding to a W-box TTGAC (C/T) to orchestrate cotton resistance to Verticillium dahliae |
| title_sort | ghwrky75 positively regulates ghpr6 5b via binding to a w box ttgac c t to orchestrate cotton resistance to verticillium dahliae |
| topic | cotton proteinase inhibitors WRKY transcription factor Verticillium wilt |
| url | http://www.sciencedirect.com/science/article/pii/S2095311924002089 |
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