LipidIN: a comprehensive repository for flash platform-independent annotation and reverse lipidomics

Abstract Improving annotation accuracy, coverage, speed and depth of lipid profiles remains a significant challenge in traditional lipid annotation. We introduce LipidIN, an advanced framework designed for flash platform-independent annotation. LipidIN features a 168.5-million lipid fragmentation hi...

Full description

Saved in:
Bibliographic Details
Main Authors: Hao Xu, Tianhang Jiang, Yuxiang Lin, Lei Zhang, Huan Yang, Xiaoyun Huang, Ridong Mao, Zhu Yang, Changchun Zeng, Shuang Zhao, Lijun Di, Wenbin Zhang, Jun Zeng, Zongwei Cai, Shu-Hai Lin
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-59683-5
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849310323790577664
author Hao Xu
Tianhang Jiang
Yuxiang Lin
Lei Zhang
Huan Yang
Xiaoyun Huang
Ridong Mao
Zhu Yang
Changchun Zeng
Shuang Zhao
Lijun Di
Wenbin Zhang
Jun Zeng
Zongwei Cai
Shu-Hai Lin
author_facet Hao Xu
Tianhang Jiang
Yuxiang Lin
Lei Zhang
Huan Yang
Xiaoyun Huang
Ridong Mao
Zhu Yang
Changchun Zeng
Shuang Zhao
Lijun Di
Wenbin Zhang
Jun Zeng
Zongwei Cai
Shu-Hai Lin
author_sort Hao Xu
collection DOAJ
description Abstract Improving annotation accuracy, coverage, speed and depth of lipid profiles remains a significant challenge in traditional lipid annotation. We introduce LipidIN, an advanced framework designed for flash platform-independent annotation. LipidIN features a 168.5-million lipid fragmentation hierarchical library that encompasses all potential chain compositions and carbon-carbon double bond locations. The expeditious querying module achieves speeds exceeding one hundred billion queries per second across all mass spectral libraries. The lipid categories intelligence model is developed using three relative retention time rules, reducing false positive annotations and predicting unannotated lipids with a 5.7% estimated false discovery rate, covering 8923 lipids cross various species. More importantly, LipidIN integrates a Wide-spectrum Modeling Yield network for regenerating lipid fragment fingerprints to further improve accuracy and coverage with a 20% estimated recall boosting. We further demonstrate the utility of LipidIN in multiple tasks for lipid annotation and biomarker discovery in clinical cohorts.
format Article
id doaj-art-ee5faf47eb1e44a2a30af4f45b586e6f
institution Kabale University
issn 2041-1723
language English
publishDate 2025-05-01
publisher Nature Portfolio
record_format Article
series Nature Communications
spelling doaj-art-ee5faf47eb1e44a2a30af4f45b586e6f2025-08-20T03:53:46ZengNature PortfolioNature Communications2041-17232025-05-0116111710.1038/s41467-025-59683-5LipidIN: a comprehensive repository for flash platform-independent annotation and reverse lipidomicsHao Xu0Tianhang Jiang1Yuxiang Lin2Lei Zhang3Huan Yang4Xiaoyun Huang5Ridong Mao6Zhu Yang7Changchun Zeng8Shuang Zhao9Lijun Di10Wenbin Zhang11Jun Zeng12Zongwei Cai13Shu-Hai Lin14The First Affiliated Hospital of Xiamen University, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, XMU-HBN Skin Biomedical Research Center, Xiamen UniversityThe First Affiliated Hospital of Xiamen University, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, XMU-HBN Skin Biomedical Research Center, Xiamen UniversityDepartment of Breast Surgery, Fujian Medical University Union HospitalThe First Affiliated Hospital of Xiamen University, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, XMU-HBN Skin Biomedical Research Center, Xiamen UniversityThe First Affiliated Hospital of Xiamen University, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, XMU-HBN Skin Biomedical Research Center, Xiamen UniversityCollege of Ocean Food and Biological Engineering, Jimei UniversityThe First Affiliated Hospital of Xiamen University, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, XMU-HBN Skin Biomedical Research Center, Xiamen UniversityState Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist UniversityDepartment of General Medicine, Shenzhen Longhua District Central HospitalXiamen Meliomics Co., Ltd.Department of Biological Sciences, Faculty of Health Sciences, University of MacauDepartment of Occupational and Environmental Health and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical UniversityCollege of Ocean Food and Biological Engineering, Jimei UniversityState Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist UniversityThe First Affiliated Hospital of Xiamen University, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, XMU-HBN Skin Biomedical Research Center, Xiamen UniversityAbstract Improving annotation accuracy, coverage, speed and depth of lipid profiles remains a significant challenge in traditional lipid annotation. We introduce LipidIN, an advanced framework designed for flash platform-independent annotation. LipidIN features a 168.5-million lipid fragmentation hierarchical library that encompasses all potential chain compositions and carbon-carbon double bond locations. The expeditious querying module achieves speeds exceeding one hundred billion queries per second across all mass spectral libraries. The lipid categories intelligence model is developed using three relative retention time rules, reducing false positive annotations and predicting unannotated lipids with a 5.7% estimated false discovery rate, covering 8923 lipids cross various species. More importantly, LipidIN integrates a Wide-spectrum Modeling Yield network for regenerating lipid fragment fingerprints to further improve accuracy and coverage with a 20% estimated recall boosting. We further demonstrate the utility of LipidIN in multiple tasks for lipid annotation and biomarker discovery in clinical cohorts.https://doi.org/10.1038/s41467-025-59683-5
spellingShingle Hao Xu
Tianhang Jiang
Yuxiang Lin
Lei Zhang
Huan Yang
Xiaoyun Huang
Ridong Mao
Zhu Yang
Changchun Zeng
Shuang Zhao
Lijun Di
Wenbin Zhang
Jun Zeng
Zongwei Cai
Shu-Hai Lin
LipidIN: a comprehensive repository for flash platform-independent annotation and reverse lipidomics
Nature Communications
title LipidIN: a comprehensive repository for flash platform-independent annotation and reverse lipidomics
title_full LipidIN: a comprehensive repository for flash platform-independent annotation and reverse lipidomics
title_fullStr LipidIN: a comprehensive repository for flash platform-independent annotation and reverse lipidomics
title_full_unstemmed LipidIN: a comprehensive repository for flash platform-independent annotation and reverse lipidomics
title_short LipidIN: a comprehensive repository for flash platform-independent annotation and reverse lipidomics
title_sort lipidin a comprehensive repository for flash platform independent annotation and reverse lipidomics
url https://doi.org/10.1038/s41467-025-59683-5
work_keys_str_mv AT haoxu lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT tianhangjiang lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT yuxianglin lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT leizhang lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT huanyang lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT xiaoyunhuang lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT ridongmao lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT zhuyang lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT changchunzeng lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT shuangzhao lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT lijundi lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT wenbinzhang lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT junzeng lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT zongweicai lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics
AT shuhailin lipidinacomprehensiverepositoryforflashplatformindependentannotationandreverselipidomics