Hsa_circRNA_100791 Modulates Trim13 Through Sponging miR-487b-5p to Facilitate Inflammation in Allergic Rhinitis
Jianhua Wu,1,2 Yisha Wu,1 Peng Jin,3 Xiaoxue Zi,3 Yuzhu Wan,1 Kaiyue Sun,1,4 Li Shi,1,3 Deyun Wang5 1Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial ENT Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, People’s Republic of China; 2Department of Oto...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2024-12-01
|
| Series: | Journal of Inflammation Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/hsacircrna100791-modulates-trim13-through-sponging-mir-487b-5p-to-faci-peer-reviewed-fulltext-article-JIR |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850249929465790464 |
|---|---|
| author | Wu J Wu Y Jin P Zi X Wan Y Sun K Shi L Wang D |
| author_facet | Wu J Wu Y Jin P Zi X Wan Y Sun K Shi L Wang D |
| author_sort | Wu J |
| collection | DOAJ |
| description | Jianhua Wu,1,2 Yisha Wu,1 Peng Jin,3 Xiaoxue Zi,3 Yuzhu Wan,1 Kaiyue Sun,1,4 Li Shi,1,3 Deyun Wang5 1Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial ENT Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, People’s Republic of China; 2Department of Otolaryngology Head and Neck Surgery, Binzhou People’s Hospital, Binzhou, Shandong, People’s Republic of China; 3Department of Otolaryngology, The Second Hospital of Shandong University, Jinan, Shandong, People’s Republic of China; 4Department of Physiology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, People’s Republic of China; 5Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeCorrespondence: Li Shi, Department of Otolaryngology, The Second Hospital of Shandong University, Jinan, Shandong, 250033, People’s Republic of China, Tel +86 0531 83086278, Fax +86 0531 83086279, Email shili126@sina.com Kaiyue Sun, Department of Physiology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250022, People’s Republic of China, Tel +86 0531 83086277, Fax +86 0531 83086279, Email 18354159626@163.comBackground: Circular RNAs (circRNAs) are a novel class of endogenous non-coding RNA molecules in eukaryotes, involved in many essential biological processes. However, their role in allergic rhinitis (AR) has not been extensively studied.Methods: The expression levels of hsa_circRNA_100791 were measured using qRT-PCR in peripheral blood mononuclear cells (PBMCs) and nasal mucosa from AR patients. The biological function of hsa_circRNA_100791 in AR was investigated through RNA-seq and a series of in vitro experiments. Western blotting, luciferase reporter assays, and rescue experiments were conducted to elucidate the molecular mechanisms underlying hsa_circRNA_100791. Additionally, a mouse model was used to assess the functional role of hsa_circRNA_100791 in vivo.Results: Upregulation of hsa_circRNA_100791 was observed in both PBMCs and nasal mucosa of AR patients. In vitro, increased expression of hsa_circRNA_100791 promoted the production of pro-inflammatory mediators (IL-1β, IL-4, IL-5, IL-6, IL-8, IL-13, IL-17, IL-18, IL-33, TNF-α, and NF-κB) and inhibited IL-2 and IFN-γ. Conversely, knockdown of hsa_circRNA_100791 both in vitro and in vivo alleviated AR symptoms, reduced pro-inflammatory mediators, and enhanced IL-2 and IFN-γ levels. Mechanistically, we found hsa_circRNA_100791 contributing to the pathological processes of AR, which upregulate TRIM13 via sponging miR-487b-5p.Conclusion: Our study demonstrated that hsa_circRNA_100791 mitigates the inhibitory effect of miR-487b-5p on Trim13 by directly binding to miR-487b-5p. This interaction regulates the expression of inflammatory factors and facilitates AR. Thus, hsa_circRNA_100791 could be a promising new therapeutic target for AR.Keywords: allergic rhinitis, hsa_circ_100791, miR-487b-5p, Trim13, diagnosis, inflammation, NF-κB |
| format | Article |
| id | doaj-art-ee5e6031879b49ed99cd970d6a820c63 |
| institution | OA Journals |
| issn | 1178-7031 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Inflammation Research |
| spelling | doaj-art-ee5e6031879b49ed99cd970d6a820c632025-08-20T01:58:22ZengDove Medical PressJournal of Inflammation Research1178-70312024-12-01Volume 17111751119398439Hsa_circRNA_100791 Modulates Trim13 Through Sponging miR-487b-5p to Facilitate Inflammation in Allergic RhinitisWu JWu YJin PZi XWan YSun KShi LWang DJianhua Wu,1,2 Yisha Wu,1 Peng Jin,3 Xiaoxue Zi,3 Yuzhu Wan,1 Kaiyue Sun,1,4 Li Shi,1,3 Deyun Wang5 1Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial ENT Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, People’s Republic of China; 2Department of Otolaryngology Head and Neck Surgery, Binzhou People’s Hospital, Binzhou, Shandong, People’s Republic of China; 3Department of Otolaryngology, The Second Hospital of Shandong University, Jinan, Shandong, People’s Republic of China; 4Department of Physiology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, People’s Republic of China; 5Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeCorrespondence: Li Shi, Department of Otolaryngology, The Second Hospital of Shandong University, Jinan, Shandong, 250033, People’s Republic of China, Tel +86 0531 83086278, Fax +86 0531 83086279, Email shili126@sina.com Kaiyue Sun, Department of Physiology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250022, People’s Republic of China, Tel +86 0531 83086277, Fax +86 0531 83086279, Email 18354159626@163.comBackground: Circular RNAs (circRNAs) are a novel class of endogenous non-coding RNA molecules in eukaryotes, involved in many essential biological processes. However, their role in allergic rhinitis (AR) has not been extensively studied.Methods: The expression levels of hsa_circRNA_100791 were measured using qRT-PCR in peripheral blood mononuclear cells (PBMCs) and nasal mucosa from AR patients. The biological function of hsa_circRNA_100791 in AR was investigated through RNA-seq and a series of in vitro experiments. Western blotting, luciferase reporter assays, and rescue experiments were conducted to elucidate the molecular mechanisms underlying hsa_circRNA_100791. Additionally, a mouse model was used to assess the functional role of hsa_circRNA_100791 in vivo.Results: Upregulation of hsa_circRNA_100791 was observed in both PBMCs and nasal mucosa of AR patients. In vitro, increased expression of hsa_circRNA_100791 promoted the production of pro-inflammatory mediators (IL-1β, IL-4, IL-5, IL-6, IL-8, IL-13, IL-17, IL-18, IL-33, TNF-α, and NF-κB) and inhibited IL-2 and IFN-γ. Conversely, knockdown of hsa_circRNA_100791 both in vitro and in vivo alleviated AR symptoms, reduced pro-inflammatory mediators, and enhanced IL-2 and IFN-γ levels. Mechanistically, we found hsa_circRNA_100791 contributing to the pathological processes of AR, which upregulate TRIM13 via sponging miR-487b-5p.Conclusion: Our study demonstrated that hsa_circRNA_100791 mitigates the inhibitory effect of miR-487b-5p on Trim13 by directly binding to miR-487b-5p. This interaction regulates the expression of inflammatory factors and facilitates AR. Thus, hsa_circRNA_100791 could be a promising new therapeutic target for AR.Keywords: allergic rhinitis, hsa_circ_100791, miR-487b-5p, Trim13, diagnosis, inflammation, NF-κBhttps://www.dovepress.com/hsacircrna100791-modulates-trim13-through-sponging-mir-487b-5p-to-faci-peer-reviewed-fulltext-article-JIRallergic rhinitishsa_circ_100791mir-487b-5ptrim13diagnosisinflammationnf-κb. |
| spellingShingle | Wu J Wu Y Jin P Zi X Wan Y Sun K Shi L Wang D Hsa_circRNA_100791 Modulates Trim13 Through Sponging miR-487b-5p to Facilitate Inflammation in Allergic Rhinitis Journal of Inflammation Research allergic rhinitis hsa_circ_100791 mir-487b-5p trim13 diagnosis inflammation nf-κb. |
| title | Hsa_circRNA_100791 Modulates Trim13 Through Sponging miR-487b-5p to Facilitate Inflammation in Allergic Rhinitis |
| title_full | Hsa_circRNA_100791 Modulates Trim13 Through Sponging miR-487b-5p to Facilitate Inflammation in Allergic Rhinitis |
| title_fullStr | Hsa_circRNA_100791 Modulates Trim13 Through Sponging miR-487b-5p to Facilitate Inflammation in Allergic Rhinitis |
| title_full_unstemmed | Hsa_circRNA_100791 Modulates Trim13 Through Sponging miR-487b-5p to Facilitate Inflammation in Allergic Rhinitis |
| title_short | Hsa_circRNA_100791 Modulates Trim13 Through Sponging miR-487b-5p to Facilitate Inflammation in Allergic Rhinitis |
| title_sort | hsa circrna 100791 modulates trim13 through sponging mir 487b 5p to facilitate inflammation in allergic rhinitis |
| topic | allergic rhinitis hsa_circ_100791 mir-487b-5p trim13 diagnosis inflammation nf-κb. |
| url | https://www.dovepress.com/hsacircrna100791-modulates-trim13-through-sponging-mir-487b-5p-to-faci-peer-reviewed-fulltext-article-JIR |
| work_keys_str_mv | AT wuj hsacircrna100791modulatestrim13throughspongingmir487b5ptofacilitateinflammationinallergicrhinitis AT wuy hsacircrna100791modulatestrim13throughspongingmir487b5ptofacilitateinflammationinallergicrhinitis AT jinp hsacircrna100791modulatestrim13throughspongingmir487b5ptofacilitateinflammationinallergicrhinitis AT zix hsacircrna100791modulatestrim13throughspongingmir487b5ptofacilitateinflammationinallergicrhinitis AT wany hsacircrna100791modulatestrim13throughspongingmir487b5ptofacilitateinflammationinallergicrhinitis AT sunk hsacircrna100791modulatestrim13throughspongingmir487b5ptofacilitateinflammationinallergicrhinitis AT shil hsacircrna100791modulatestrim13throughspongingmir487b5ptofacilitateinflammationinallergicrhinitis AT wangd hsacircrna100791modulatestrim13throughspongingmir487b5ptofacilitateinflammationinallergicrhinitis |