Genomic Analysis of Two Histamine-Producing Strains Isolated from Yellowfin Tuna
Psychrotrophic <i>Morganella</i> spp. is a typical histamine producer commonly found in seafood, exhibiting a high histamine-producing capacity. In this study, two strains of <i>Morganella</i> (GWT 902 and GWT 904) isolated from yellowfin tuna were subjected to phenotypic and...
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2025-04-01
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| author | Yazhe Wang Di Wang Shengjun Chen Gang Yu Zhenhua Ma Ya Wei Chunsheng Li Yueqi Wang Chaoming Shen Yongqiang Zhao |
| author_facet | Yazhe Wang Di Wang Shengjun Chen Gang Yu Zhenhua Ma Ya Wei Chunsheng Li Yueqi Wang Chaoming Shen Yongqiang Zhao |
| author_sort | Yazhe Wang |
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| description | Psychrotrophic <i>Morganella</i> spp. is a typical histamine producer commonly found in seafood, exhibiting a high histamine-producing capacity. In this study, two strains of <i>Morganella</i> (GWT 902 and GWT 904) isolated from yellowfin tuna were subjected to phenotypic and genotypic characterization. Phenotypic analysis reveals differences in growth temperature, NaCl tolerance, and D-galactose fermentation capacity between the two strains. Notably, the histamine production capacity of GWT 902 is significantly higher than that of GWT 904 at 4 °C. The complete genome sequences of strains GWT 902 and GWT 904 were sequenced, identifying GWT 902 as <i>Morganella psychrotolerans</i> and GWT 904 as <i>Morganella morganii</i> subsp. <i>sibonii</i>. Genomic analysis confirms the presence of histidine decarboxylase gene clusters (<i>hdcT1</i>, <i>hdc</i>, <i>hdcT2</i>, <i>hisRS</i>) in both strains, and sequence alignment shows that the amino acid sequence similarity of histidine decarboxylase encoded by the <i>hdc</i> gene was 95.24%. Gene function analysis further identified genes associated with putrescine biosynthesis, sulfur metabolism, lipase and protease secretion, and detected key genes in quorum sensing (QS), stress adaptation, and antibiotic resistance. This study provides valuable insights into the taxonomic analysis of psychrotrophic <i>Morganella</i> spp. and contributes to the development of efficient strategies for preventing histamine formation in seafood. |
| format | Article |
| id | doaj-art-ee59b7996a7f4d4aa75fdd9e988edc85 |
| institution | DOAJ |
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| language | English |
| publishDate | 2025-04-01 |
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| series | Foods |
| spelling | doaj-art-ee59b7996a7f4d4aa75fdd9e988edc852025-08-20T02:59:14ZengMDPI AGFoods2304-81582025-04-01149153210.3390/foods14091532Genomic Analysis of Two Histamine-Producing Strains Isolated from Yellowfin TunaYazhe Wang0Di Wang1Shengjun Chen2Gang Yu3Zhenhua Ma4Ya Wei5Chunsheng Li6Yueqi Wang7Chaoming Shen8Yongqiang Zhao9College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya 572426, ChinaKey Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya 572426, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaBeihai Product Quality Testing Institute, Beihai 536000, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaPsychrotrophic <i>Morganella</i> spp. is a typical histamine producer commonly found in seafood, exhibiting a high histamine-producing capacity. In this study, two strains of <i>Morganella</i> (GWT 902 and GWT 904) isolated from yellowfin tuna were subjected to phenotypic and genotypic characterization. Phenotypic analysis reveals differences in growth temperature, NaCl tolerance, and D-galactose fermentation capacity between the two strains. Notably, the histamine production capacity of GWT 902 is significantly higher than that of GWT 904 at 4 °C. The complete genome sequences of strains GWT 902 and GWT 904 were sequenced, identifying GWT 902 as <i>Morganella psychrotolerans</i> and GWT 904 as <i>Morganella morganii</i> subsp. <i>sibonii</i>. Genomic analysis confirms the presence of histidine decarboxylase gene clusters (<i>hdcT1</i>, <i>hdc</i>, <i>hdcT2</i>, <i>hisRS</i>) in both strains, and sequence alignment shows that the amino acid sequence similarity of histidine decarboxylase encoded by the <i>hdc</i> gene was 95.24%. Gene function analysis further identified genes associated with putrescine biosynthesis, sulfur metabolism, lipase and protease secretion, and detected key genes in quorum sensing (QS), stress adaptation, and antibiotic resistance. This study provides valuable insights into the taxonomic analysis of psychrotrophic <i>Morganella</i> spp. and contributes to the development of efficient strategies for preventing histamine formation in seafood.https://www.mdpi.com/2304-8158/14/9/1532<i>Morganella psychrotolerans</i><i>Morganella morganii</i> subsp. <i>Sibonii</i>spoilagewhole genome sequencing |
| spellingShingle | Yazhe Wang Di Wang Shengjun Chen Gang Yu Zhenhua Ma Ya Wei Chunsheng Li Yueqi Wang Chaoming Shen Yongqiang Zhao Genomic Analysis of Two Histamine-Producing Strains Isolated from Yellowfin Tuna Foods <i>Morganella psychrotolerans</i> <i>Morganella morganii</i> subsp. <i>Sibonii</i> spoilage whole genome sequencing |
| title | Genomic Analysis of Two Histamine-Producing Strains Isolated from Yellowfin Tuna |
| title_full | Genomic Analysis of Two Histamine-Producing Strains Isolated from Yellowfin Tuna |
| title_fullStr | Genomic Analysis of Two Histamine-Producing Strains Isolated from Yellowfin Tuna |
| title_full_unstemmed | Genomic Analysis of Two Histamine-Producing Strains Isolated from Yellowfin Tuna |
| title_short | Genomic Analysis of Two Histamine-Producing Strains Isolated from Yellowfin Tuna |
| title_sort | genomic analysis of two histamine producing strains isolated from yellowfin tuna |
| topic | <i>Morganella psychrotolerans</i> <i>Morganella morganii</i> subsp. <i>Sibonii</i> spoilage whole genome sequencing |
| url | https://www.mdpi.com/2304-8158/14/9/1532 |
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