Diversity of the Obligate Gut Bacteria Indicates Host–Symbiont Coevolution at the Population Level in the Plataspid Stinkbug Megacopta cribraria
ABSTRACT Ishikawaella is an obligate gut bacterium in stinkbugs that belong to Plataspidae family (Hemiptera: Heteroptera). It is vertically transmitted to newborn nymphs through capsules laid on eggs by maternal stinkbugs. Previous research has established a pattern of strict cospeciation between P...
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Wiley
2024-11-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.70611 |
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| author | Yuan Wang Xiu‐Xiu Zhu Bo‐Ren Xiao Xin‐Rui Hou Yu‐Xin Liu Jia‐Yue Zhou Yi‐Peng Ren Wen‐Jun Bu Huai‐Jun Xue |
| author_facet | Yuan Wang Xiu‐Xiu Zhu Bo‐Ren Xiao Xin‐Rui Hou Yu‐Xin Liu Jia‐Yue Zhou Yi‐Peng Ren Wen‐Jun Bu Huai‐Jun Xue |
| author_sort | Yuan Wang |
| collection | DOAJ |
| description | ABSTRACT Ishikawaella is an obligate gut bacterium in stinkbugs that belong to Plataspidae family (Hemiptera: Heteroptera). It is vertically transmitted to newborn nymphs through capsules laid on eggs by maternal stinkbugs. Previous research has established a pattern of strict cospeciation between Plataspidae stinkbugs and Ishikawaella. However, the possibility of host–symbiont coevolution at the population level within Plataspidae stinkbugs has not been thoroughly explored. This study analyzed the samples of Megacopta cribraria from three phylogenetic clades to investigate host–symbiont coevolution in this insect species. We compared data from third‐generation sequencing (PacBio), next‐generation sequencing (Illumina), and first‐generation sequencing (Sanger), and the results indicated that Illumina sequencing most accurately represents the composition of gut bacterial communities. All stinkbug individuals shared a dominant amplicon sequence variant (ASV), which accounted for an average of 65.99% of Ishikawaella sequences (ranging from 58.68% to 87.01%). The top five ASVs (ASV0–ASV4) represented 99.82% of all Ishikawaella sequences. Among these, the number of base substitutions between any two ASVs ranged from 1 to 3, significantly lower than the number of substitutions between the main and minor ASVs. This finding suggests that closely related strains are likely to coexist in the same host. Beta diversity analyses revealed significant differences in Ishikawaella composition among the three phylogenetic clades, providing evidence for host–symbiont coevolution at the population level in Plataspidae stinkbugs. |
| format | Article |
| id | doaj-art-ee566f76c40347b3bb77faf52fbd9ffc |
| institution | OA Journals |
| issn | 2045-7758 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj-art-ee566f76c40347b3bb77faf52fbd9ffc2025-08-20T02:34:35ZengWileyEcology and Evolution2045-77582024-11-011411n/an/a10.1002/ece3.70611Diversity of the Obligate Gut Bacteria Indicates Host–Symbiont Coevolution at the Population Level in the Plataspid Stinkbug Megacopta cribrariaYuan Wang0Xiu‐Xiu Zhu1Bo‐Ren Xiao2Xin‐Rui Hou3Yu‐Xin Liu4Jia‐Yue Zhou5Yi‐Peng Ren6Wen‐Jun Bu7Huai‐Jun Xue8Institute of Entomology, College of Life Sciences Nankai University Tianjin ChinaInstitute of Entomology, College of Life Sciences Nankai University Tianjin ChinaInstitute of Entomology, College of Life Sciences Nankai University Tianjin ChinaInstitute of Entomology, College of Life Sciences Nankai University Tianjin ChinaInstitute of Entomology, College of Life Sciences Nankai University Tianjin ChinaInstitute of Entomology, College of Life Sciences Nankai University Tianjin ChinaInstitute of Entomology, College of Life Sciences Nankai University Tianjin ChinaInstitute of Entomology, College of Life Sciences Nankai University Tianjin ChinaInstitute of Entomology, College of Life Sciences Nankai University Tianjin ChinaABSTRACT Ishikawaella is an obligate gut bacterium in stinkbugs that belong to Plataspidae family (Hemiptera: Heteroptera). It is vertically transmitted to newborn nymphs through capsules laid on eggs by maternal stinkbugs. Previous research has established a pattern of strict cospeciation between Plataspidae stinkbugs and Ishikawaella. However, the possibility of host–symbiont coevolution at the population level within Plataspidae stinkbugs has not been thoroughly explored. This study analyzed the samples of Megacopta cribraria from three phylogenetic clades to investigate host–symbiont coevolution in this insect species. We compared data from third‐generation sequencing (PacBio), next‐generation sequencing (Illumina), and first‐generation sequencing (Sanger), and the results indicated that Illumina sequencing most accurately represents the composition of gut bacterial communities. All stinkbug individuals shared a dominant amplicon sequence variant (ASV), which accounted for an average of 65.99% of Ishikawaella sequences (ranging from 58.68% to 87.01%). The top five ASVs (ASV0–ASV4) represented 99.82% of all Ishikawaella sequences. Among these, the number of base substitutions between any two ASVs ranged from 1 to 3, significantly lower than the number of substitutions between the main and minor ASVs. This finding suggests that closely related strains are likely to coexist in the same host. Beta diversity analyses revealed significant differences in Ishikawaella composition among the three phylogenetic clades, providing evidence for host–symbiont coevolution at the population level in Plataspidae stinkbugs.https://doi.org/10.1002/ece3.70611amplicon sequencingbeta diversitycloneHemipteraIshikawaella |
| spellingShingle | Yuan Wang Xiu‐Xiu Zhu Bo‐Ren Xiao Xin‐Rui Hou Yu‐Xin Liu Jia‐Yue Zhou Yi‐Peng Ren Wen‐Jun Bu Huai‐Jun Xue Diversity of the Obligate Gut Bacteria Indicates Host–Symbiont Coevolution at the Population Level in the Plataspid Stinkbug Megacopta cribraria Ecology and Evolution amplicon sequencing beta diversity clone Hemiptera Ishikawaella |
| title | Diversity of the Obligate Gut Bacteria Indicates Host–Symbiont Coevolution at the Population Level in the Plataspid Stinkbug Megacopta cribraria |
| title_full | Diversity of the Obligate Gut Bacteria Indicates Host–Symbiont Coevolution at the Population Level in the Plataspid Stinkbug Megacopta cribraria |
| title_fullStr | Diversity of the Obligate Gut Bacteria Indicates Host–Symbiont Coevolution at the Population Level in the Plataspid Stinkbug Megacopta cribraria |
| title_full_unstemmed | Diversity of the Obligate Gut Bacteria Indicates Host–Symbiont Coevolution at the Population Level in the Plataspid Stinkbug Megacopta cribraria |
| title_short | Diversity of the Obligate Gut Bacteria Indicates Host–Symbiont Coevolution at the Population Level in the Plataspid Stinkbug Megacopta cribraria |
| title_sort | diversity of the obligate gut bacteria indicates host symbiont coevolution at the population level in the plataspid stinkbug megacopta cribraria |
| topic | amplicon sequencing beta diversity clone Hemiptera Ishikawaella |
| url | https://doi.org/10.1002/ece3.70611 |
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