How Long Do Microorganisms Survive and Persist in Food? A Systematic Review

Foodborne illnesses caused by microorganisms pose a significant threat to public health. Understanding the survival and persistence of these microorganisms in various food matrices is crucial for developing effective control strategies. This systematic review aims to address the current knowledge ga...

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Main Authors: Eric S. Donkor, Famous K. Sosah, Alex Odoom, Bernard T. Odai, Angela Parry-Hanson Kunadu
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/4/901
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author Eric S. Donkor
Famous K. Sosah
Alex Odoom
Bernard T. Odai
Angela Parry-Hanson Kunadu
author_facet Eric S. Donkor
Famous K. Sosah
Alex Odoom
Bernard T. Odai
Angela Parry-Hanson Kunadu
author_sort Eric S. Donkor
collection DOAJ
description Foodborne illnesses caused by microorganisms pose a significant threat to public health. Understanding the survival and persistence of these microorganisms in various food matrices is crucial for developing effective control strategies. This systematic review aims to address the current knowledge gaps related to the duration of survival and persistence of microbial pathogens in food, as well as the impact of external environmental conditions on their viability. A comprehensive search was conducted across major databases, including studies published until 3 June 2024. The PRISMA guidelines were followed to ensure a systematic and transparent approach. Foodborne bacteria, such as <i>Salmonella</i> spp., <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i> O157:H7, were found to persist for extended durations, ranging from days to over a year. The mean duration of persistence for all of the bacteria was 246 days, whereas the survival duration was 16 days. Bacterial survival and persistence were significantly influenced by temperature, with warmer conditions (>25 °C) generally supporting longer persistence. Relative humidity also played a role, with low-humidity environments (<50% RH) favouring the survival of pathogens like <i>Listeria monocytogenes</i> and <i>Escherichia coli</i>. In contrast, viruses, such as hepatitis A virus and Human norovirus, showed only survival patterns, with average durations of 21 days and temperature being the primary environmental factor influencing their survival. Overall, this review provides evidence that a wide range of microbial pathogens, including <i>Escherichia coli</i> O157:H7, <i>Salmonella</i> spp., <i>Listeria monocytogenes</i>, and the hepatitis A virus, can survive and persist in food for prolonged periods, leading to potential harm. These insights underscore the necessity of stringent food safety measures and continuous monitoring to mitigate the risks posed by these resilient pathogens, contributing to a safer and more secure food supply chain.
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spelling doaj-art-ee4f76fde5ee44819407d862f0671e652025-08-20T03:13:45ZengMDPI AGMicroorganisms2076-26072025-04-0113490110.3390/microorganisms13040901How Long Do Microorganisms Survive and Persist in Food? A Systematic ReviewEric S. Donkor0Famous K. Sosah1Alex Odoom2Bernard T. Odai3Angela Parry-Hanson Kunadu4Department of Medical Microbiology, University of Ghana Medical School, Korle Bu, Accra P.O. Box KB 4236, GhanaDepartment of Medical Microbiology, University of Ghana Medical School, Korle Bu, Accra P.O. Box KB 4236, GhanaDepartment of Medical Microbiology, University of Ghana Medical School, Korle Bu, Accra P.O. Box KB 4236, GhanaRadiation Technology Centre, Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, Accra P.O. Box LG 80, GhanaDepartment of Nutrition and Food Science, University of Ghana, Accra P.O. Box LG 134, GhanaFoodborne illnesses caused by microorganisms pose a significant threat to public health. Understanding the survival and persistence of these microorganisms in various food matrices is crucial for developing effective control strategies. This systematic review aims to address the current knowledge gaps related to the duration of survival and persistence of microbial pathogens in food, as well as the impact of external environmental conditions on their viability. A comprehensive search was conducted across major databases, including studies published until 3 June 2024. The PRISMA guidelines were followed to ensure a systematic and transparent approach. Foodborne bacteria, such as <i>Salmonella</i> spp., <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i> O157:H7, were found to persist for extended durations, ranging from days to over a year. The mean duration of persistence for all of the bacteria was 246 days, whereas the survival duration was 16 days. Bacterial survival and persistence were significantly influenced by temperature, with warmer conditions (>25 °C) generally supporting longer persistence. Relative humidity also played a role, with low-humidity environments (<50% RH) favouring the survival of pathogens like <i>Listeria monocytogenes</i> and <i>Escherichia coli</i>. In contrast, viruses, such as hepatitis A virus and Human norovirus, showed only survival patterns, with average durations of 21 days and temperature being the primary environmental factor influencing their survival. Overall, this review provides evidence that a wide range of microbial pathogens, including <i>Escherichia coli</i> O157:H7, <i>Salmonella</i> spp., <i>Listeria monocytogenes</i>, and the hepatitis A virus, can survive and persist in food for prolonged periods, leading to potential harm. These insights underscore the necessity of stringent food safety measures and continuous monitoring to mitigate the risks posed by these resilient pathogens, contributing to a safer and more secure food supply chain.https://www.mdpi.com/2076-2607/13/4/901persistencesurvivalpathogensenvironmental factorsfood supply chainfood safety
spellingShingle Eric S. Donkor
Famous K. Sosah
Alex Odoom
Bernard T. Odai
Angela Parry-Hanson Kunadu
How Long Do Microorganisms Survive and Persist in Food? A Systematic Review
Microorganisms
persistence
survival
pathogens
environmental factors
food supply chain
food safety
title How Long Do Microorganisms Survive and Persist in Food? A Systematic Review
title_full How Long Do Microorganisms Survive and Persist in Food? A Systematic Review
title_fullStr How Long Do Microorganisms Survive and Persist in Food? A Systematic Review
title_full_unstemmed How Long Do Microorganisms Survive and Persist in Food? A Systematic Review
title_short How Long Do Microorganisms Survive and Persist in Food? A Systematic Review
title_sort how long do microorganisms survive and persist in food a systematic review
topic persistence
survival
pathogens
environmental factors
food supply chain
food safety
url https://www.mdpi.com/2076-2607/13/4/901
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