The simultaneous determination of lycopene and β-carotene in fruit vegetables and plasma by HPLC
To establish a rapid determination method of lycopene and β-carotene in fruit vegetables and plasma, different pretreatments were used for different samples. Separation was achieved by the use of ODS-C<sub>18</sub> column, acetonitrile: tetrathydrofuran (60∶40, V/V) as mobile phase, a fl...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2000-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2000.04.0444 |
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| Summary: | To establish a rapid determination method of lycopene and β-carotene in fruit vegetables and plasma, different pretreatments were used for different samples. Separation was achieved by the use of ODS-C<sub>18</sub> column, acetonitrile: tetrathydrofuran (60∶40, V/V) as mobile phase, a flow rate of 1.0 mL/min and detector at 445 nm was used. The recoveries of lycopene and β-carotene were 89.54%-93.71% and 90.29%-96.43% respectively, the coefficients of variation were 3.9%-5.68%. The results showed that this method was simple, rapid and accurate. |
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| ISSN: | 1008-9209 2097-5155 |