Multi-omics analysis elucidates phased defense and resource allocation trade-offs in fusarium resistance of maize

Fusarium graminearum-induced ear rot may lead to severe yield losses and mycotoxin contamination, which threaten global maize production. To dissect resistance mechanisms, we integrated a genome-wide association study (GWAS) of 420 maize inbred lines across five environments with a time-resolved tra...

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Main Authors: Aiguo Su, Senlin Xiao, Zhiyong Li, Sairu Duan, Shuaishuai Wang, Haixia Zhang, Ruyang Zhang, Jinfeng Xing, Chunhui Li, Xiaqing Wang, Yanxin Zhao, Shuai Wang, Xuan Sun, Fengge Wang, Yang Yang, Yuandong Wang, Tianjun Xu, Xueyuan Zhang, Ronghuan Wang, Wei Song, Jiuran Zhao
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Plant Stress
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Online Access:http://www.sciencedirect.com/science/article/pii/S2667064X25002453
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author Aiguo Su
Senlin Xiao
Zhiyong Li
Sairu Duan
Shuaishuai Wang
Haixia Zhang
Ruyang Zhang
Jinfeng Xing
Chunhui Li
Xiaqing Wang
Yanxin Zhao
Shuai Wang
Xuan Sun
Fengge Wang
Yang Yang
Yuandong Wang
Tianjun Xu
Xueyuan Zhang
Ronghuan Wang
Wei Song
Jiuran Zhao
author_facet Aiguo Su
Senlin Xiao
Zhiyong Li
Sairu Duan
Shuaishuai Wang
Haixia Zhang
Ruyang Zhang
Jinfeng Xing
Chunhui Li
Xiaqing Wang
Yanxin Zhao
Shuai Wang
Xuan Sun
Fengge Wang
Yang Yang
Yuandong Wang
Tianjun Xu
Xueyuan Zhang
Ronghuan Wang
Wei Song
Jiuran Zhao
author_sort Aiguo Su
collection DOAJ
description Fusarium graminearum-induced ear rot may lead to severe yield losses and mycotoxin contamination, which threaten global maize production. To dissect resistance mechanisms, we integrated a genome-wide association study (GWAS) of 420 maize inbred lines across five environments with a time-resolved transcriptomics analysis of resistant and susceptible genotypes. On the basis of GWAS, 151 significant single nucleotide polymorphisms (SNP) were identified, including novel loci in bin 7.04 and known resistance hotspots in bin 3.04. By comparing the transcriptomes of resistant (X178) and susceptible (B73) lines during early infection phases, we detected 1537 differentially expressed genes associated with pathways related to plant immune responses (e.g., defense signaling, secondary metabolism, redox homeostasis, and cytoskeletal reorganization). Additionally, 32 potential resistance genes were differentially expressed according to our transcriptome analysis, which enabled the prioritization of candidate genes, including ZmTRX (thioredoxin), ZmGuLO (ascorbate biosynthesis), and ZmVOZ1 (transcription factor). We propose that resistant maize lines have phased defense responses that transiently suppress the synthesis of storage-related proteins (e.g., α-zein), reallocate resources for immunity-related activities, and balance stress response-associated trade-offs via dynamic regulation.
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spelling doaj-art-ee2aed6f6e514e5588e798d6ed3b641a2025-08-23T04:49:44ZengElsevierPlant Stress2667-064X2025-09-011710097710.1016/j.stress.2025.100977Multi-omics analysis elucidates phased defense and resource allocation trade-offs in fusarium resistance of maizeAiguo Su0Senlin Xiao1Zhiyong Li2Sairu Duan3Shuaishuai Wang4Haixia Zhang5Ruyang Zhang6Jinfeng Xing7Chunhui Li8Xiaqing Wang9Yanxin Zhao10Shuai Wang11Xuan Sun12Fengge Wang13Yang Yang14Yuandong Wang15Tianjun Xu16Xueyuan Zhang17Ronghuan Wang18Wei Song19Jiuran Zhao20Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaBeijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaCorresponding authors.; Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaCorresponding authors.; Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaCorresponding authors.; Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Maize Research Institute, No. 11, Middle Shuguang Garden Road, Haidian District, Beijing, 100097, ChinaFusarium graminearum-induced ear rot may lead to severe yield losses and mycotoxin contamination, which threaten global maize production. To dissect resistance mechanisms, we integrated a genome-wide association study (GWAS) of 420 maize inbred lines across five environments with a time-resolved transcriptomics analysis of resistant and susceptible genotypes. On the basis of GWAS, 151 significant single nucleotide polymorphisms (SNP) were identified, including novel loci in bin 7.04 and known resistance hotspots in bin 3.04. By comparing the transcriptomes of resistant (X178) and susceptible (B73) lines during early infection phases, we detected 1537 differentially expressed genes associated with pathways related to plant immune responses (e.g., defense signaling, secondary metabolism, redox homeostasis, and cytoskeletal reorganization). Additionally, 32 potential resistance genes were differentially expressed according to our transcriptome analysis, which enabled the prioritization of candidate genes, including ZmTRX (thioredoxin), ZmGuLO (ascorbate biosynthesis), and ZmVOZ1 (transcription factor). We propose that resistant maize lines have phased defense responses that transiently suppress the synthesis of storage-related proteins (e.g., α-zein), reallocate resources for immunity-related activities, and balance stress response-associated trade-offs via dynamic regulation.http://www.sciencedirect.com/science/article/pii/S2667064X25002453MaizeEar rotFusarium graminearumResistance genesDefense response
spellingShingle Aiguo Su
Senlin Xiao
Zhiyong Li
Sairu Duan
Shuaishuai Wang
Haixia Zhang
Ruyang Zhang
Jinfeng Xing
Chunhui Li
Xiaqing Wang
Yanxin Zhao
Shuai Wang
Xuan Sun
Fengge Wang
Yang Yang
Yuandong Wang
Tianjun Xu
Xueyuan Zhang
Ronghuan Wang
Wei Song
Jiuran Zhao
Multi-omics analysis elucidates phased defense and resource allocation trade-offs in fusarium resistance of maize
Plant Stress
Maize
Ear rot
Fusarium graminearum
Resistance genes
Defense response
title Multi-omics analysis elucidates phased defense and resource allocation trade-offs in fusarium resistance of maize
title_full Multi-omics analysis elucidates phased defense and resource allocation trade-offs in fusarium resistance of maize
title_fullStr Multi-omics analysis elucidates phased defense and resource allocation trade-offs in fusarium resistance of maize
title_full_unstemmed Multi-omics analysis elucidates phased defense and resource allocation trade-offs in fusarium resistance of maize
title_short Multi-omics analysis elucidates phased defense and resource allocation trade-offs in fusarium resistance of maize
title_sort multi omics analysis elucidates phased defense and resource allocation trade offs in fusarium resistance of maize
topic Maize
Ear rot
Fusarium graminearum
Resistance genes
Defense response
url http://www.sciencedirect.com/science/article/pii/S2667064X25002453
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