Antimicrobial Resistance Properties, Biofilm, and mecA Gene Presence in Staphylococcus Aureus Isolated from Raw Milk Sold in Van, Türkiye
Staphylococcus aureus can cause foodborne poisoning and can form biofilms, reducing enterotoxin production and the penetration rate of antibiotics. Therefore, infections and poisonings caused by S. aureus can be difficult to treat. The aim of this study was to investigate the antibiotic resistance l...
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Hasan Eleroğlu
2023-02-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/5817 |
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| author | Rabia Mehtap Tuncay Yakup Can Sancak |
| author_facet | Rabia Mehtap Tuncay Yakup Can Sancak |
| author_sort | Rabia Mehtap Tuncay |
| collection | DOAJ |
| description | Staphylococcus aureus can cause foodborne poisoning and can form biofilms, reducing enterotoxin production and the penetration rate of antibiotics. Therefore, infections and poisonings caused by S. aureus can be difficult to treat. The aim of this study was to investigate the antibiotic resistance levels of S. aureus isolates obtained from raw milk and the presence of biofilm and mecA gene and to reveal the risk to public health. S. aureus was isolated in 30 (30%) of 100 raw milk samples obtained from Van province. A total of 48 S. aureus isolates were obtained from 30 samples. All 48 isolates (100%) obtained were resistant to penicillin G and cefoxitin, 4 (8.33%) to sulfamethoxazole-trimethoprim and chloramphenicol, and 25 (52.08%) to erythromycin. All of the isolates (100%) were found to be susceptible to ceftriaxone. In addition, 26 (54.16%) of the obtained isolates were found to be resistant to at least 3 antibiotics. The strains found to be resistant to penicillin and cefoxitin were also intermediate to at least one of the antibiotics. Biofilm genes were detected in 18 of the S. aureus isolates (37.5%). Twelve of the biofilm-forming isolates contain icaA (66.6%), 3 contain icaD (16.6%) and the other 3 contain bap genes (16.6%). Three of the isolates contain icaA and icaD genes and the other three isolates contain icaA and bap genes together. It was determined that only 2 of the isolates contained the mecA gene. The isolates containing the mecA gene also contained the icaA and icaD genes. In conclusion, the fact that S. aureus isolates had high antibiotic resistance, biofilm-forming genes, and methicillin resistance genes showed that raw milk may be a serious public health problem. |
| format | Article |
| id | doaj-art-ee29cd699b4f488e9c64c298d10235e7 |
| institution | OA Journals |
| issn | 2148-127X |
| language | English |
| publishDate | 2023-02-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-ee29cd699b4f488e9c64c298d10235e72025-08-20T02:04:31ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2023-02-0111235536210.24925/turjaf.v11i2.355-362.58174562Antimicrobial Resistance Properties, Biofilm, and mecA Gene Presence in Staphylococcus Aureus Isolated from Raw Milk Sold in Van, TürkiyeRabia Mehtap Tuncay0https://orcid.org/0000-0002-3510-5369Yakup Can Sancak1https://orcid.org/0000-0003-4490-9606Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Van Yüzüncü Yıl University, 65080 VanDepartment of Food Hygiene and Technology, Faculty of Veterinary Medicine, Van Yüzüncü Yıl University, 65080 VanStaphylococcus aureus can cause foodborne poisoning and can form biofilms, reducing enterotoxin production and the penetration rate of antibiotics. Therefore, infections and poisonings caused by S. aureus can be difficult to treat. The aim of this study was to investigate the antibiotic resistance levels of S. aureus isolates obtained from raw milk and the presence of biofilm and mecA gene and to reveal the risk to public health. S. aureus was isolated in 30 (30%) of 100 raw milk samples obtained from Van province. A total of 48 S. aureus isolates were obtained from 30 samples. All 48 isolates (100%) obtained were resistant to penicillin G and cefoxitin, 4 (8.33%) to sulfamethoxazole-trimethoprim and chloramphenicol, and 25 (52.08%) to erythromycin. All of the isolates (100%) were found to be susceptible to ceftriaxone. In addition, 26 (54.16%) of the obtained isolates were found to be resistant to at least 3 antibiotics. The strains found to be resistant to penicillin and cefoxitin were also intermediate to at least one of the antibiotics. Biofilm genes were detected in 18 of the S. aureus isolates (37.5%). Twelve of the biofilm-forming isolates contain icaA (66.6%), 3 contain icaD (16.6%) and the other 3 contain bap genes (16.6%). Three of the isolates contain icaA and icaD genes and the other three isolates contain icaA and bap genes together. It was determined that only 2 of the isolates contained the mecA gene. The isolates containing the mecA gene also contained the icaA and icaD genes. In conclusion, the fact that S. aureus isolates had high antibiotic resistance, biofilm-forming genes, and methicillin resistance genes showed that raw milk may be a serious public health problem.http://www.agrifoodscience.com/index.php/TURJAF/article/view/5817biofilm genesmecastaphylococcus aureus |
| spellingShingle | Rabia Mehtap Tuncay Yakup Can Sancak Antimicrobial Resistance Properties, Biofilm, and mecA Gene Presence in Staphylococcus Aureus Isolated from Raw Milk Sold in Van, Türkiye Turkish Journal of Agriculture: Food Science and Technology biofilm genes meca staphylococcus aureus |
| title | Antimicrobial Resistance Properties, Biofilm, and mecA Gene Presence in Staphylococcus Aureus Isolated from Raw Milk Sold in Van, Türkiye |
| title_full | Antimicrobial Resistance Properties, Biofilm, and mecA Gene Presence in Staphylococcus Aureus Isolated from Raw Milk Sold in Van, Türkiye |
| title_fullStr | Antimicrobial Resistance Properties, Biofilm, and mecA Gene Presence in Staphylococcus Aureus Isolated from Raw Milk Sold in Van, Türkiye |
| title_full_unstemmed | Antimicrobial Resistance Properties, Biofilm, and mecA Gene Presence in Staphylococcus Aureus Isolated from Raw Milk Sold in Van, Türkiye |
| title_short | Antimicrobial Resistance Properties, Biofilm, and mecA Gene Presence in Staphylococcus Aureus Isolated from Raw Milk Sold in Van, Türkiye |
| title_sort | antimicrobial resistance properties biofilm and meca gene presence in staphylococcus aureus isolated from raw milk sold in van turkiye |
| topic | biofilm genes meca staphylococcus aureus |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/5817 |
| work_keys_str_mv | AT rabiamehtaptuncay antimicrobialresistancepropertiesbiofilmandmecagenepresenceinstaphylococcusaureusisolatedfromrawmilksoldinvanturkiye AT yakupcansancak antimicrobialresistancepropertiesbiofilmandmecagenepresenceinstaphylococcusaureusisolatedfromrawmilksoldinvanturkiye |