Phylogenetic analysis of the microbial mat in the hot spring Garga (Baikal rift zone) and the diversity of natural peptidases
Hydrolytic bacteria (in particular, proteolytics) are the primary destructors in hot springs. The proteolytic bacteria are able to secrete enzymes that are active in wide ranges of pH and temperature. The aim of this work was to study the taxonomic composition, the structure of the bacterial microbi...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2018-01-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/1274 |
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| author | E. V. Lavrentyeva A. A. Radnagurueva T. G. Banzaraktsaeva S. M. Bazarov D. D. Barkhutova I. D. Ulzetueva M. K. Chernyavsky M. R. Kabilov V. V. Khakhinov |
| author_facet | E. V. Lavrentyeva A. A. Radnagurueva T. G. Banzaraktsaeva S. M. Bazarov D. D. Barkhutova I. D. Ulzetueva M. K. Chernyavsky M. R. Kabilov V. V. Khakhinov |
| author_sort | E. V. Lavrentyeva |
| collection | DOAJ |
| description | Hydrolytic bacteria (in particular, proteolytics) are the primary destructors in hot springs. The proteolytic bacteria are able to secrete enzymes that are active in wide ranges of pH and temperature. The aim of this work was to study the taxonomic composition, the structure of the bacterial microbial mat, and to study the distribution of peptidases in the thermophilic microbial Garga community. For the study, we sampled the microbial mat at a water temperature of 54.2 °C and a pH of 8.3. Hydrochemical analysis of water showed a high content of sulfates, 390 mg/dm3. The microelement composition of water showed that the Garga water had increased concentrations of B, Rb, Li, Ba, Sr. We analyzed the taxonomic diversity of the microbial community in the hot spring Garga at a temperature zone of 54 °C. The structure of the microbial mat is represented by various phylogenetic groups of mesophilic and thermophilic bacteria, with various metabolic and ecological functions. The dominant group in this community was the phylum Firmicutes (64 %). The analysis of the collected metagenomic sequences of the microbial community allowed the detected peptidases in the microbial community in the hot spring Garga to be for the first time systematized and characterized. Comparisons of metagenomic sequences of representative data showed a dominance of serine peptidase class enzymes. Natural peptidases in the investigated microbial community ensure the hydrolysis of biopolymers at the first stages of the destruction of organic matter and may have biotechnological relevance. |
| format | Article |
| id | doaj-art-ee0a93ea2d6d46bcb607aa57ea478d47 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-ee0a93ea2d6d46bcb607aa57ea478d472025-02-01T09:58:05ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592018-01-0121895996310.18699/VJ17.319708Phylogenetic analysis of the microbial mat in the hot spring Garga (Baikal rift zone) and the diversity of natural peptidasesE. V. Lavrentyeva0A. A. Radnagurueva1T. G. Banzaraktsaeva2S. M. Bazarov3D. D. Barkhutova4I. D. Ulzetueva5M. K. Chernyavsky6M. R. Kabilov7V. V. Khakhinov8Institute of General and Experimental Biology SB RAS; Buryat State University.Institute of General and Experimental Biology SB RAS.Institute of General and Experimental Biology SB RAS.Institute of General and Experimental Biology SB RAS.Institute of General and Experimental Biology SB RAS.Baikal Institute of Nature Management SB RAS.Geological Institute SB RAS.Institute of Chemical Biology and Fundamental Medicine SB RAS.Buryat State University.Hydrolytic bacteria (in particular, proteolytics) are the primary destructors in hot springs. The proteolytic bacteria are able to secrete enzymes that are active in wide ranges of pH and temperature. The aim of this work was to study the taxonomic composition, the structure of the bacterial microbial mat, and to study the distribution of peptidases in the thermophilic microbial Garga community. For the study, we sampled the microbial mat at a water temperature of 54.2 °C and a pH of 8.3. Hydrochemical analysis of water showed a high content of sulfates, 390 mg/dm3. The microelement composition of water showed that the Garga water had increased concentrations of B, Rb, Li, Ba, Sr. We analyzed the taxonomic diversity of the microbial community in the hot spring Garga at a temperature zone of 54 °C. The structure of the microbial mat is represented by various phylogenetic groups of mesophilic and thermophilic bacteria, with various metabolic and ecological functions. The dominant group in this community was the phylum Firmicutes (64 %). The analysis of the collected metagenomic sequences of the microbial community allowed the detected peptidases in the microbial community in the hot spring Garga to be for the first time systematized and characterized. Comparisons of metagenomic sequences of representative data showed a dominance of serine peptidase class enzymes. Natural peptidases in the investigated microbial community ensure the hydrolysis of biopolymers at the first stages of the destruction of organic matter and may have biotechnological relevance.https://vavilov.elpub.ru/jour/article/view/1274phylogenetic analysismicrobial communitymetagenomic analysispeptidasesbaikal rift zone |
| spellingShingle | E. V. Lavrentyeva A. A. Radnagurueva T. G. Banzaraktsaeva S. M. Bazarov D. D. Barkhutova I. D. Ulzetueva M. K. Chernyavsky M. R. Kabilov V. V. Khakhinov Phylogenetic analysis of the microbial mat in the hot spring Garga (Baikal rift zone) and the diversity of natural peptidases Вавиловский журнал генетики и селекции phylogenetic analysis microbial community metagenomic analysis peptidases baikal rift zone |
| title | Phylogenetic analysis of the microbial mat in the hot spring Garga (Baikal rift zone) and the diversity of natural peptidases |
| title_full | Phylogenetic analysis of the microbial mat in the hot spring Garga (Baikal rift zone) and the diversity of natural peptidases |
| title_fullStr | Phylogenetic analysis of the microbial mat in the hot spring Garga (Baikal rift zone) and the diversity of natural peptidases |
| title_full_unstemmed | Phylogenetic analysis of the microbial mat in the hot spring Garga (Baikal rift zone) and the diversity of natural peptidases |
| title_short | Phylogenetic analysis of the microbial mat in the hot spring Garga (Baikal rift zone) and the diversity of natural peptidases |
| title_sort | phylogenetic analysis of the microbial mat in the hot spring garga baikal rift zone and the diversity of natural peptidases |
| topic | phylogenetic analysis microbial community metagenomic analysis peptidases baikal rift zone |
| url | https://vavilov.elpub.ru/jour/article/view/1274 |
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