Denitrifying Dephosphatation via Nitrite under Anoxic Conditions

Denitrifying dephosphatation is a cost-effective method for simultaneously removing nitrogen and phosphorus due to its lower chemical oxygen demand (COD) requirement, reduced aeration, and decreased sludge production. In batch experiments, denitrifying dephosphatation of 20 ± 1 mg PO4-P/l using nitr...

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Main Authors: Andrijana Brozinčević, Dijana Grgas, Irena Landeka Jurčević, Mirna Habuda-Stanić, Bruno Zelić, Tibela Landeka Dragičević
Format: Article
Language:English
Published: Croatian Society of Chemical Engineers 2025-03-01
Series:Kemija u Industriji
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Online Access:http://silverstripe.fkit.hr/kui/assets/Uploads/3-109-116-KUI-3-4-2025.pdf
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author Andrijana Brozinčević
Dijana Grgas
Irena Landeka Jurčević
Mirna Habuda-Stanić
Bruno Zelić
Tibela Landeka Dragičević
author_facet Andrijana Brozinčević
Dijana Grgas
Irena Landeka Jurčević
Mirna Habuda-Stanić
Bruno Zelić
Tibela Landeka Dragičević
author_sort Andrijana Brozinčević
collection DOAJ
description Denitrifying dephosphatation is a cost-effective method for simultaneously removing nitrogen and phosphorus due to its lower chemical oxygen demand (COD) requirement, reduced aeration, and decreased sludge production. In batch experiments, denitrifying dephosphatation of 20 ± 1 mg PO4-P/l using nitrite was studied at N/P ratios of 2, 3, and 4. The effects of both limiting and non-limiting COD, provided by sodium acetate as a carbon source, on the activity of denitrifying phosphorus-accumulating organisms (DPAOs) were investigated. In all batch tests, at ratios N/P of 2, 3, 4, increasing the C/N ratio led to higher NO2-N and P removal rates and efficiencies. In all batch tests, at ratios N/P of 2, 3, 4, the highest denitrification and anoxic phosphorus uptake rates were recorded under the following conditions: at C/N ratio of 4 and N/P ratio of 2, C/N ratio of 3 and N/P ratio of 3, and C/N ratio of 2 and N/P ratio of 4. In tests with limited carbon sources (N/P of 2 and C/N < 4, N/P of 3 and C/N < 3, and N/P of 4 and C/N < 2), both denitrification and P uptake rates and efficiencies increased with rising C/N ratios. However, the batch tests with COD overdose resulted in increased denitrification rate but deteriorated P removal rate and efficiency. In the tests with a C/N ratio of 7, the P removal efficiencies were 75 ± 3 %, 68 ± 2 %, and 62 ± 4 % at N/P ratios of 2, 3, and 4, respectively.
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spelling doaj-art-edfee46bd5ff4b468eefffb771bdafeb2025-08-20T02:56:32ZengCroatian Society of Chemical EngineersKemija u Industriji0022-98301334-90902025-03-01743-410911610.15255/KUI.2024.034Denitrifying Dephosphatation via Nitrite under Anoxic ConditionsAndrijana Brozinčević0Dijana Grgas1Irena Landeka Jurčević2Mirna Habuda-Stanić3Bruno Zelić4Tibela Landeka Dragičević5Plitvice Lakes National Park, Dr. Ivo Pevalek Scientific Research Centre, Josipa Jovića 19, 53 231 Plitvička Jezera, CroatiaUniversity of Zagreb, Faculty of Food Technology and Biotechnology, Pierottijeva 6, 10 000 Zagreb, CroatiaUniversity of Zagreb, Faculty of Food Technology and Biotechnology, Pierottijeva 6, 10 000 Zagreb, CroatiaJ. J. Strossmayer University of Osijek, Faculty of Food Technology Osijek, F. Kuhača 18, 31 000 Osijek, CroatiaUniversity of Zagreb Faculty of Chemical Engineering and Technology, Trg Marka Marulića 19, 10 000 Zagreb, CroatiaUniversity of Zagreb, Faculty of Food Technology and Biotechnology, Pierottijeva 6, 10 000 Zagreb, CroatiaDenitrifying dephosphatation is a cost-effective method for simultaneously removing nitrogen and phosphorus due to its lower chemical oxygen demand (COD) requirement, reduced aeration, and decreased sludge production. In batch experiments, denitrifying dephosphatation of 20 ± 1 mg PO4-P/l using nitrite was studied at N/P ratios of 2, 3, and 4. The effects of both limiting and non-limiting COD, provided by sodium acetate as a carbon source, on the activity of denitrifying phosphorus-accumulating organisms (DPAOs) were investigated. In all batch tests, at ratios N/P of 2, 3, 4, increasing the C/N ratio led to higher NO2-N and P removal rates and efficiencies. In all batch tests, at ratios N/P of 2, 3, 4, the highest denitrification and anoxic phosphorus uptake rates were recorded under the following conditions: at C/N ratio of 4 and N/P ratio of 2, C/N ratio of 3 and N/P ratio of 3, and C/N ratio of 2 and N/P ratio of 4. In tests with limited carbon sources (N/P of 2 and C/N < 4, N/P of 3 and C/N < 3, and N/P of 4 and C/N < 2), both denitrification and P uptake rates and efficiencies increased with rising C/N ratios. However, the batch tests with COD overdose resulted in increased denitrification rate but deteriorated P removal rate and efficiency. In the tests with a C/N ratio of 7, the P removal efficiencies were 75 ± 3 %, 68 ± 2 %, and 62 ± 4 % at N/P ratios of 2, 3, and 4, respectively.http://silverstripe.fkit.hr/kui/assets/Uploads/3-109-116-KUI-3-4-2025.pdfdenitrificationnitritephosphate uptakeanoxic conditionsdenitrifying phosphorus-accumulating organisms
spellingShingle Andrijana Brozinčević
Dijana Grgas
Irena Landeka Jurčević
Mirna Habuda-Stanić
Bruno Zelić
Tibela Landeka Dragičević
Denitrifying Dephosphatation via Nitrite under Anoxic Conditions
Kemija u Industriji
denitrification
nitrite
phosphate uptake
anoxic conditions
denitrifying phosphorus-accumulating organisms
title Denitrifying Dephosphatation via Nitrite under Anoxic Conditions
title_full Denitrifying Dephosphatation via Nitrite under Anoxic Conditions
title_fullStr Denitrifying Dephosphatation via Nitrite under Anoxic Conditions
title_full_unstemmed Denitrifying Dephosphatation via Nitrite under Anoxic Conditions
title_short Denitrifying Dephosphatation via Nitrite under Anoxic Conditions
title_sort denitrifying dephosphatation via nitrite under anoxic conditions
topic denitrification
nitrite
phosphate uptake
anoxic conditions
denitrifying phosphorus-accumulating organisms
url http://silverstripe.fkit.hr/kui/assets/Uploads/3-109-116-KUI-3-4-2025.pdf
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