Tight coupling of metabolic oscillations and intracellular water dynamics in Saccharomyces cerevisiae.
We detected very strong coupling between the oscillating concentration of ATP and the dynamics of intracellular water during glycolysis in Saccharomyces cerevisiae. Our results indicate that: i) dipolar relaxation of intracellular water is heterogeneous within the cell and different from dilute cond...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117308&type=printable |
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| author | Henrik Seir Thoke Asger Tobiesen Jonathan Brewer Per Lyngs Hansen Roberto P Stock Lars F Olsen Luis A Bagatolli |
| author_facet | Henrik Seir Thoke Asger Tobiesen Jonathan Brewer Per Lyngs Hansen Roberto P Stock Lars F Olsen Luis A Bagatolli |
| author_sort | Henrik Seir Thoke |
| collection | DOAJ |
| description | We detected very strong coupling between the oscillating concentration of ATP and the dynamics of intracellular water during glycolysis in Saccharomyces cerevisiae. Our results indicate that: i) dipolar relaxation of intracellular water is heterogeneous within the cell and different from dilute conditions, ii) water dipolar relaxation oscillates with glycolysis and in phase with ATP concentration, iii) this phenomenon is scale-invariant from the subcellular to the ensemble of synchronized cells and, iv) the periodicity of both glycolytic oscillations and dipolar relaxation are equally affected by D2O in a dose-dependent manner. These results offer a new insight into the coupling of an emergent intensive physicochemical property of the cell, i.e. cell-wide water dipolar relaxation, and a central metabolite (ATP) produced by a robustly oscillating metabolic process. |
| format | Article |
| id | doaj-art-edfb4d577a5642818d75f9ce41bf375a |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-edfb4d577a5642818d75f9ce41bf375a2025-08-20T03:01:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011730810.1371/journal.pone.0117308Tight coupling of metabolic oscillations and intracellular water dynamics in Saccharomyces cerevisiae.Henrik Seir ThokeAsger TobiesenJonathan BrewerPer Lyngs HansenRoberto P StockLars F OlsenLuis A BagatolliWe detected very strong coupling between the oscillating concentration of ATP and the dynamics of intracellular water during glycolysis in Saccharomyces cerevisiae. Our results indicate that: i) dipolar relaxation of intracellular water is heterogeneous within the cell and different from dilute conditions, ii) water dipolar relaxation oscillates with glycolysis and in phase with ATP concentration, iii) this phenomenon is scale-invariant from the subcellular to the ensemble of synchronized cells and, iv) the periodicity of both glycolytic oscillations and dipolar relaxation are equally affected by D2O in a dose-dependent manner. These results offer a new insight into the coupling of an emergent intensive physicochemical property of the cell, i.e. cell-wide water dipolar relaxation, and a central metabolite (ATP) produced by a robustly oscillating metabolic process.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117308&type=printable |
| spellingShingle | Henrik Seir Thoke Asger Tobiesen Jonathan Brewer Per Lyngs Hansen Roberto P Stock Lars F Olsen Luis A Bagatolli Tight coupling of metabolic oscillations and intracellular water dynamics in Saccharomyces cerevisiae. PLoS ONE |
| title | Tight coupling of metabolic oscillations and intracellular water dynamics in Saccharomyces cerevisiae. |
| title_full | Tight coupling of metabolic oscillations and intracellular water dynamics in Saccharomyces cerevisiae. |
| title_fullStr | Tight coupling of metabolic oscillations and intracellular water dynamics in Saccharomyces cerevisiae. |
| title_full_unstemmed | Tight coupling of metabolic oscillations and intracellular water dynamics in Saccharomyces cerevisiae. |
| title_short | Tight coupling of metabolic oscillations and intracellular water dynamics in Saccharomyces cerevisiae. |
| title_sort | tight coupling of metabolic oscillations and intracellular water dynamics in saccharomyces cerevisiae |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117308&type=printable |
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