Calpastatin and myostatin genes polymorphism and their association with slaughter traits in the Gashun Soviet Merino breed grown in southern Russia
The study aimed to investigate the genetic variability of the Gashun Soviet Merino sheep breed, determining myostatin (MSTN) and calpastatin (CAST) gene polymorphism by the PCR-RFLP method. Earmark samples were collected from 200 animals, and DNA was isolated using a commercial kit. The MSTN gene ha...
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| Format: | Article |
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University of Mosul, College of Veterinary Medicine
2025-04-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_186960_14c6f1bc6ecdeb57d9be67d3149767c3.pdf |
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| author | Nadezhda V. Shirokova Vladimir K. Fiodorov Izabella G. Kazarova Ivan F. Gorlov Marina I. Slozhenkina Elena Y. Anisimova Ekaterina V. Karpenko |
| author_facet | Nadezhda V. Shirokova Vladimir K. Fiodorov Izabella G. Kazarova Ivan F. Gorlov Marina I. Slozhenkina Elena Y. Anisimova Ekaterina V. Karpenko |
| author_sort | Nadezhda V. Shirokova |
| collection | DOAJ |
| description | The study aimed to investigate the genetic variability of the Gashun Soviet Merino sheep breed, determining myostatin (MSTN) and calpastatin (CAST) gene polymorphism by the PCR-RFLP method. Earmark samples were collected from 200 animals, and DNA was isolated using a commercial kit. The MSTN gene had A and G allele frequencies of 0.33 and 0.67, and the CAST gene had M and N allele frequencies of 0.83 and 0.17, respectively. The frequencies of the AG and GG genotypes for MSTN and MM and MN for CAST were 0.66 and 0.34, respectively, in both. Genetic equilibrium was maintained for the CAST gene (χ²=4.1951). For the MSTN gene, the Hardy-Weinberg equilibrium was violated (χ²=24.2). The pre-slaughter body weight, hot carcass weight and slaughter weight were higher in CAST_MN genotype than CAST _MM by 4.8%, 6.5%, and 6.4%. The hot carcass and slaughter weights of lambs with the MSTN_AG genotype were higher than MSTN _GG by 4.7% in both. However, the pre-slaughter weight in the animals with the MSTN _GG genotype was higher by 3.4%. Data on the CAST and MSTN gene polymorphism and the genetic structure of the studied sheep population were obtained for the first time, their association with slaughter traits was revealed, and desirable genotypes CAST_MN and MSTN_AG were determined. The Na, Ne, Ho, He, FIS and χ2 values obtained and the absence of individuals with CAST_NN and MSTN_AA genotypes substantiated the prospect of further use of these genes in programs of genetic livestock improvement. |
| format | Article |
| id | doaj-art-edfaf7eaba67458e9550ea80b58c6468 |
| institution | Kabale University |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| publishDate | 2025-04-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj-art-edfaf7eaba67458e9550ea80b58c64682025-08-20T03:48:31ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552025-04-0139223324110.33899/ijvs.2024.154790.4000186960Calpastatin and myostatin genes polymorphism and their association with slaughter traits in the Gashun Soviet Merino breed grown in southern RussiaNadezhda V. Shirokova0Vladimir K. Fiodorov1Izabella G. Kazarova2Ivan F. Gorlov3Marina I. Slozhenkina4Elena Y. Anisimova5Ekaterina V. Karpenko6Laboratory of Molecular-Genetic Diagnostics, Don State Agrarian University, Rostov-on-Don, Russian FederationLaboratory of Molecular-Genetic Diagnostics, Don State Agrarian University, Rostov-on-Don, Russian FederationLaboratory of Molecular-Genetic Diagnostics, Don State Agrarian University, Rostov-on-Don, Russian FederationVolgograd State Agrarian University, Volgograd, Russian FederationPovolzhsky Research Institute of Manufacture and Processing of Meat and Dairy Product, Volgograd, Russian FederationPovolzhsky Research Institute of Manufacture and Processing of Meat and Dairy Product, Volgograd, Russian FederationPovolzhsky Research Institute of Manufacture and Processing of Meat and Dairy Product, Volgograd, Russian FederationThe study aimed to investigate the genetic variability of the Gashun Soviet Merino sheep breed, determining myostatin (MSTN) and calpastatin (CAST) gene polymorphism by the PCR-RFLP method. Earmark samples were collected from 200 animals, and DNA was isolated using a commercial kit. The MSTN gene had A and G allele frequencies of 0.33 and 0.67, and the CAST gene had M and N allele frequencies of 0.83 and 0.17, respectively. The frequencies of the AG and GG genotypes for MSTN and MM and MN for CAST were 0.66 and 0.34, respectively, in both. Genetic equilibrium was maintained for the CAST gene (χ²=4.1951). For the MSTN gene, the Hardy-Weinberg equilibrium was violated (χ²=24.2). The pre-slaughter body weight, hot carcass weight and slaughter weight were higher in CAST_MN genotype than CAST _MM by 4.8%, 6.5%, and 6.4%. The hot carcass and slaughter weights of lambs with the MSTN_AG genotype were higher than MSTN _GG by 4.7% in both. However, the pre-slaughter weight in the animals with the MSTN _GG genotype was higher by 3.4%. Data on the CAST and MSTN gene polymorphism and the genetic structure of the studied sheep population were obtained for the first time, their association with slaughter traits was revealed, and desirable genotypes CAST_MN and MSTN_AG were determined. The Na, Ne, Ho, He, FIS and χ2 values obtained and the absence of individuals with CAST_NN and MSTN_AA genotypes substantiated the prospect of further use of these genes in programs of genetic livestock improvement.https://www.vetmedmosul.com/article_186960_14c6f1bc6ecdeb57d9be67d3149767c3.pdfgenetic variationmeat productivitypcr-rflpsheep breedingsnp |
| spellingShingle | Nadezhda V. Shirokova Vladimir K. Fiodorov Izabella G. Kazarova Ivan F. Gorlov Marina I. Slozhenkina Elena Y. Anisimova Ekaterina V. Karpenko Calpastatin and myostatin genes polymorphism and their association with slaughter traits in the Gashun Soviet Merino breed grown in southern Russia Iraqi Journal of Veterinary Sciences genetic variation meat productivity pcr-rflp sheep breeding snp |
| title | Calpastatin and myostatin genes polymorphism and their association with slaughter traits in the Gashun Soviet Merino breed grown in southern Russia |
| title_full | Calpastatin and myostatin genes polymorphism and their association with slaughter traits in the Gashun Soviet Merino breed grown in southern Russia |
| title_fullStr | Calpastatin and myostatin genes polymorphism and their association with slaughter traits in the Gashun Soviet Merino breed grown in southern Russia |
| title_full_unstemmed | Calpastatin and myostatin genes polymorphism and their association with slaughter traits in the Gashun Soviet Merino breed grown in southern Russia |
| title_short | Calpastatin and myostatin genes polymorphism and their association with slaughter traits in the Gashun Soviet Merino breed grown in southern Russia |
| title_sort | calpastatin and myostatin genes polymorphism and their association with slaughter traits in the gashun soviet merino breed grown in southern russia |
| topic | genetic variation meat productivity pcr-rflp sheep breeding snp |
| url | https://www.vetmedmosul.com/article_186960_14c6f1bc6ecdeb57d9be67d3149767c3.pdf |
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