Effects of Dietary Pretreatment with All-<i>trans</i> Lycopene on Lipopolysaccharide-Induced Jejunal Inflammation: A Multi-Pathway Phenomenon
This study was conducted to investigate the effects and mechanisms of all-<i>trans</i> lycopene on intestinal health by establishing lipopolysaccharide-induced (LPS-induced) jejunal inflammation model. Dietary lycopene supplementation enhanced serum and jejunum antioxidant capacity. Lyco...
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MDPI AG
2025-02-01
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| Online Access: | https://www.mdpi.com/2304-8158/14/5/794 |
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| author | Daolin Mou Dajiang Ding Junning Pu Pan Zhou Enming Cao Xueyan Zhang Junrong Lan Lu Ye Wanxue Wen |
| author_facet | Daolin Mou Dajiang Ding Junning Pu Pan Zhou Enming Cao Xueyan Zhang Junrong Lan Lu Ye Wanxue Wen |
| author_sort | Daolin Mou |
| collection | DOAJ |
| description | This study was conducted to investigate the effects and mechanisms of all-<i>trans</i> lycopene on intestinal health by establishing lipopolysaccharide-induced (LPS-induced) jejunal inflammation model. Dietary lycopene supplementation enhanced serum and jejunum antioxidant capacity. Lycopene significantly reduced LPS-induced upregulation of toll-like receptor-4 (TLR-4) and nuclear factor kappa-B (NF-κB), suggesting that lycopene reduced the activation of TLR-4/NF-κB signaling pathway induced by LPS challenge, and further protected mice from LPS induced jejunal inflammation. Furthermore, lycopene increased jejunal zonula occludens-1 (ZO-1) protein expression that was reduced by LPS challenge, and increased abundance of <i>Rikenella</i>, <i>Lachnospiraceae_NK4A136_group</i> and <i>Mucispirillum</i> potentially associated with reducing gut inflammation. Overall, these results showed that pretreatment with lycopene can improve jejunal inflammation and ensure intestinal health in mice by improving antioxidant capacity, intestinal barrier function, microorganisms potentially associated with anti-inflammatory effects and reducing the activation of TLR-4/NF-κB signaling pathway by LPS. We provided a new insight into lycopene prevented LPS-induced jejunal inflammation by corresponding alterations in serum metabolites and gut microbiota, improving antioxidant capacity and regulating the TLR-4/NF-κB signaling pathway in mice. |
| format | Article |
| id | doaj-art-edf19eb0248b4bd1a34f8c729c32e4bb |
| institution | DOAJ |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-edf19eb0248b4bd1a34f8c729c32e4bb2025-08-20T02:52:38ZengMDPI AGFoods2304-81582025-02-0114579410.3390/foods14050794Effects of Dietary Pretreatment with All-<i>trans</i> Lycopene on Lipopolysaccharide-Induced Jejunal Inflammation: A Multi-Pathway PhenomenonDaolin Mou0Dajiang Ding1Junning Pu2Pan Zhou3Enming Cao4Xueyan Zhang5Junrong Lan6Lu Ye7Wanxue Wen8College of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, ChinaInstitute of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai 200031, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, ChinaThis study was conducted to investigate the effects and mechanisms of all-<i>trans</i> lycopene on intestinal health by establishing lipopolysaccharide-induced (LPS-induced) jejunal inflammation model. Dietary lycopene supplementation enhanced serum and jejunum antioxidant capacity. Lycopene significantly reduced LPS-induced upregulation of toll-like receptor-4 (TLR-4) and nuclear factor kappa-B (NF-κB), suggesting that lycopene reduced the activation of TLR-4/NF-κB signaling pathway induced by LPS challenge, and further protected mice from LPS induced jejunal inflammation. Furthermore, lycopene increased jejunal zonula occludens-1 (ZO-1) protein expression that was reduced by LPS challenge, and increased abundance of <i>Rikenella</i>, <i>Lachnospiraceae_NK4A136_group</i> and <i>Mucispirillum</i> potentially associated with reducing gut inflammation. Overall, these results showed that pretreatment with lycopene can improve jejunal inflammation and ensure intestinal health in mice by improving antioxidant capacity, intestinal barrier function, microorganisms potentially associated with anti-inflammatory effects and reducing the activation of TLR-4/NF-κB signaling pathway by LPS. We provided a new insight into lycopene prevented LPS-induced jejunal inflammation by corresponding alterations in serum metabolites and gut microbiota, improving antioxidant capacity and regulating the TLR-4/NF-κB signaling pathway in mice.https://www.mdpi.com/2304-8158/14/5/794lycopeneLPSintestinal inflammationantioxidant capacitymicrobiome and metabolomicsTLR4/NF-?B |
| spellingShingle | Daolin Mou Dajiang Ding Junning Pu Pan Zhou Enming Cao Xueyan Zhang Junrong Lan Lu Ye Wanxue Wen Effects of Dietary Pretreatment with All-<i>trans</i> Lycopene on Lipopolysaccharide-Induced Jejunal Inflammation: A Multi-Pathway Phenomenon Foods lycopene LPS intestinal inflammation antioxidant capacity microbiome and metabolomics TLR4/NF-?B |
| title | Effects of Dietary Pretreatment with All-<i>trans</i> Lycopene on Lipopolysaccharide-Induced Jejunal Inflammation: A Multi-Pathway Phenomenon |
| title_full | Effects of Dietary Pretreatment with All-<i>trans</i> Lycopene on Lipopolysaccharide-Induced Jejunal Inflammation: A Multi-Pathway Phenomenon |
| title_fullStr | Effects of Dietary Pretreatment with All-<i>trans</i> Lycopene on Lipopolysaccharide-Induced Jejunal Inflammation: A Multi-Pathway Phenomenon |
| title_full_unstemmed | Effects of Dietary Pretreatment with All-<i>trans</i> Lycopene on Lipopolysaccharide-Induced Jejunal Inflammation: A Multi-Pathway Phenomenon |
| title_short | Effects of Dietary Pretreatment with All-<i>trans</i> Lycopene on Lipopolysaccharide-Induced Jejunal Inflammation: A Multi-Pathway Phenomenon |
| title_sort | effects of dietary pretreatment with all i trans i lycopene on lipopolysaccharide induced jejunal inflammation a multi pathway phenomenon |
| topic | lycopene LPS intestinal inflammation antioxidant capacity microbiome and metabolomics TLR4/NF-?B |
| url | https://www.mdpi.com/2304-8158/14/5/794 |
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