A Promoter Collection for Cell‐Targeted Analysis Within the Stomatal Complex
ABSTRACT Stomatal aperture is driven by changes in turgor of the guard cells that surround the stomatal pore. Epidermal cells immediately surrounding the guard cells are thought to contribute to the kinetics of aperture changes through changes in their turgor that opposes the guard cells and thought...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2025-02-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.70045 |
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| _version_ | 1849467792902848512 |
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| author | Thanh‐Hao Nguyen Jovaras Krasauskas Thu Binh‐Anh Nguyen Azka Noureen Mark Smedley John M. Christie Wendy Harwood Michael R. Blatt Penny Hundleby |
| author_facet | Thanh‐Hao Nguyen Jovaras Krasauskas Thu Binh‐Anh Nguyen Azka Noureen Mark Smedley John M. Christie Wendy Harwood Michael R. Blatt Penny Hundleby |
| author_sort | Thanh‐Hao Nguyen |
| collection | DOAJ |
| description | ABSTRACT Stomatal aperture is driven by changes in turgor of the guard cells that surround the stomatal pore. Epidermal cells immediately surrounding the guard cells are thought to contribute to the kinetics of aperture changes through changes in their turgor that opposes the guard cells and thought their putative roles in solute storage for use by the guard cells. Nonetheless, our knowledge remains fragmentary of surrounding cell mechanics, in large part because the tools and strategies needed to target the surrounding cells independent of the guard cells are limited. Here, we have analyzed sets of promoters for Arabidopsis, Brassica, and barley that will allow physiological studies of the roles of epidermal cells and also surrounding cells in the case of barley in stomatal behavior. These tissue‐specific promoters offer distinct advantages over widely used, constitutive promoters by enabling precise and targeted gene expression within guard cells and the adjacent epidermal cells. As genetic tools, the promoters will have applications in strategies centered on physiological analyses and differential comparisons following expression targeted between the guard cells and the foliar epidermis as a whole. As such, they are well suited to questions around the mechanics of solute and water flux that will advance an understanding of the stomatal complex in these model species. |
| format | Article |
| id | doaj-art-ede4bbb69260405a93fb447eb4f2a28c |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-ede4bbb69260405a93fb447eb4f2a28c2025-08-20T03:26:04ZengWileyPlant Direct2475-44552025-02-0192n/an/a10.1002/pld3.70045A Promoter Collection for Cell‐Targeted Analysis Within the Stomatal ComplexThanh‐Hao Nguyen0Jovaras Krasauskas1Thu Binh‐Anh Nguyen2Azka Noureen3Mark Smedley4John M. Christie5Wendy Harwood6Michael R. Blatt7Penny Hundleby8Laboratory of Plant Physiology and Biophysics and the Plant Science Group, School of Molecular Biosciences, Bower Building University of Glasgow Glasgow UKJohn Innes Institute Norwich UKLaboratory of Plant Physiology and Biophysics and the Plant Science Group, School of Molecular Biosciences, Bower Building University of Glasgow Glasgow UKJohn Innes Institute Norwich UKJohn Innes Institute Norwich UKLaboratory of Plant Physiology and Biophysics and the Plant Science Group, School of Molecular Biosciences, Bower Building University of Glasgow Glasgow UKJohn Innes Institute Norwich UKLaboratory of Plant Physiology and Biophysics and the Plant Science Group, School of Molecular Biosciences, Bower Building University of Glasgow Glasgow UKJohn Innes Institute Norwich UKABSTRACT Stomatal aperture is driven by changes in turgor of the guard cells that surround the stomatal pore. Epidermal cells immediately surrounding the guard cells are thought to contribute to the kinetics of aperture changes through changes in their turgor that opposes the guard cells and thought their putative roles in solute storage for use by the guard cells. Nonetheless, our knowledge remains fragmentary of surrounding cell mechanics, in large part because the tools and strategies needed to target the surrounding cells independent of the guard cells are limited. Here, we have analyzed sets of promoters for Arabidopsis, Brassica, and barley that will allow physiological studies of the roles of epidermal cells and also surrounding cells in the case of barley in stomatal behavior. These tissue‐specific promoters offer distinct advantages over widely used, constitutive promoters by enabling precise and targeted gene expression within guard cells and the adjacent epidermal cells. As genetic tools, the promoters will have applications in strategies centered on physiological analyses and differential comparisons following expression targeted between the guard cells and the foliar epidermis as a whole. As such, they are well suited to questions around the mechanics of solute and water flux that will advance an understanding of the stomatal complex in these model species.https://doi.org/10.1002/pld3.70045guard cellfoliar epidermistissue‐specific expressionArabidopsisBrassicaHordeum |
| spellingShingle | Thanh‐Hao Nguyen Jovaras Krasauskas Thu Binh‐Anh Nguyen Azka Noureen Mark Smedley John M. Christie Wendy Harwood Michael R. Blatt Penny Hundleby A Promoter Collection for Cell‐Targeted Analysis Within the Stomatal Complex Plant Direct guard cell foliar epidermis tissue‐specific expression Arabidopsis Brassica Hordeum |
| title | A Promoter Collection for Cell‐Targeted Analysis Within the Stomatal Complex |
| title_full | A Promoter Collection for Cell‐Targeted Analysis Within the Stomatal Complex |
| title_fullStr | A Promoter Collection for Cell‐Targeted Analysis Within the Stomatal Complex |
| title_full_unstemmed | A Promoter Collection for Cell‐Targeted Analysis Within the Stomatal Complex |
| title_short | A Promoter Collection for Cell‐Targeted Analysis Within the Stomatal Complex |
| title_sort | promoter collection for cell targeted analysis within the stomatal complex |
| topic | guard cell foliar epidermis tissue‐specific expression Arabidopsis Brassica Hordeum |
| url | https://doi.org/10.1002/pld3.70045 |
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