Expression of linoleate isomerase gene from Lactobacillus reuteri PYR8 in Pichia pastoris
Linoleate isomerase gene was amplified by PCR from chromosome of Lactobacillus reuteri PYR8, then the gene was cloned into Pichia pastoris expression vector pPIC9K. The recombinant plasmid pPIC9K-LI was transformed into P. pastoris GS115 by electroporation and was induced with 1% methanol, it was de...
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| Main Authors: | ZHANG Yan-he, ZHANG Lan-wei, HU Sen, BU Zhi-gao |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2006-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2006.05.0505 |
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