Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7
Objective To investigate the effects of miR-143-3p and MAPK7 on the proliferation, migration, and invasion of U2OS human osteosarcoma (OS) cells.Methods The expression of miR-143-3p and MAPK7 in U2OS cells were detected by qRT-PCR, and the protein level of MAPK7 was measured by western blot assay. T...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1620252 |
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| author | Yu Hou Helin Feng Jianbao Jiao Ligang Qian Bo Sun Pingtao Chen Qinggui Li Zhixing Liang |
| author_facet | Yu Hou Helin Feng Jianbao Jiao Ligang Qian Bo Sun Pingtao Chen Qinggui Li Zhixing Liang |
| author_sort | Yu Hou |
| collection | DOAJ |
| description | Objective To investigate the effects of miR-143-3p and MAPK7 on the proliferation, migration, and invasion of U2OS human osteosarcoma (OS) cells.Methods The expression of miR-143-3p and MAPK7 in U2OS cells were detected by qRT-PCR, and the protein level of MAPK7 was measured by western blot assay. The targeting relationship between miR-143-3p and MAPK7 was predicted by TargetScan and verified by dual luciferase reporter assay. MTT and Transwell assays were used to detect cell viability, migrated cells and invaded cells of U2OS cells.Results Compared with hFOB1.19 cells, the expression of miR-143-3p was down-regulated and MAPK7 was up-regulated in U2OS cells. Cell viability, migration and invasion ability significantly decreased induced by miR-143-3p overexpression or MAPK7 knockdown in U2OS cells. The results of dual luciferase reporter assay indicated that miR-143-3p interacted with MAPK7. Furthermore, overexpression of MAPK7 could reverse the inhibitory effects on cell proliferation, migration and invasion in U2OS cells induced by miR-143-3p mimics.Conclusion miR-143-3p could inhibit proliferation, migration and invasion of U2OS cells by targeting MAPK7. |
| format | Article |
| id | doaj-art-edca9afa1c194a7cb8e220cbe67e885e |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-edca9afa1c194a7cb8e220cbe67e885e2025-08-20T03:51:24ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014712065207110.1080/21691401.2019.1620252Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7Yu Hou0Helin Feng1Jianbao Jiao2Ligang Qian3Bo Sun4Pingtao Chen5Qinggui Li6Zhixing Liang7Department of Orthopedics, Affiliated Hospital of Hebei University, Baoding, Hebei, ChinaDepartment of Orthopedics, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaDepartment of Orthopedics, Affiliated Hospital of Hebei University, Baoding, Hebei, ChinaDepartment of Orthopedics, Affiliated Hospital of Hebei University, Baoding, Hebei, ChinaDepartment of Orthopedics, Affiliated Hospital of Hebei University, Baoding, Hebei, ChinaDepartment of Orthopedics, Affiliated Hospital of Hebei University, Baoding, Hebei, ChinaDepartment of Orthopedics, Affiliated Hospital of Hebei University, Baoding, Hebei, ChinaDepartment of Orthopedics, Affiliated Hospital of Hebei University, Baoding, Hebei, ChinaObjective To investigate the effects of miR-143-3p and MAPK7 on the proliferation, migration, and invasion of U2OS human osteosarcoma (OS) cells.Methods The expression of miR-143-3p and MAPK7 in U2OS cells were detected by qRT-PCR, and the protein level of MAPK7 was measured by western blot assay. The targeting relationship between miR-143-3p and MAPK7 was predicted by TargetScan and verified by dual luciferase reporter assay. MTT and Transwell assays were used to detect cell viability, migrated cells and invaded cells of U2OS cells.Results Compared with hFOB1.19 cells, the expression of miR-143-3p was down-regulated and MAPK7 was up-regulated in U2OS cells. Cell viability, migration and invasion ability significantly decreased induced by miR-143-3p overexpression or MAPK7 knockdown in U2OS cells. The results of dual luciferase reporter assay indicated that miR-143-3p interacted with MAPK7. Furthermore, overexpression of MAPK7 could reverse the inhibitory effects on cell proliferation, migration and invasion in U2OS cells induced by miR-143-3p mimics.Conclusion miR-143-3p could inhibit proliferation, migration and invasion of U2OS cells by targeting MAPK7.https://www.tandfonline.com/doi/10.1080/21691401.2019.1620252MiR-143-3pmitogen-activated protein kinase 7OSmigrationinvasion |
| spellingShingle | Yu Hou Helin Feng Jianbao Jiao Ligang Qian Bo Sun Pingtao Chen Qinggui Li Zhixing Liang Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7 Artificial Cells, Nanomedicine, and Biotechnology MiR-143-3p mitogen-activated protein kinase 7 OS migration invasion |
| title | Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7 |
| title_full | Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7 |
| title_fullStr | Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7 |
| title_full_unstemmed | Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7 |
| title_short | Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7 |
| title_sort | mechanism of mir 143 3p inhibiting proliferation migration and invasion of osteosarcoma cells by targeting mapk7 |
| topic | MiR-143-3p mitogen-activated protein kinase 7 OS migration invasion |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1620252 |
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