Protocol for cryopreserving neural organoids and human living brain tissue using MEDY
Summary: Cryopreservation of neural organoids and human living brain tissue faces huge challenges. Here, we present a cryopreservation protocol for the reliable storage of diverse neural organoids and human living brain tissue using methylcellulose, ethylene glycol, DMSO, and Y27632, termed MEDY. We...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724006312 |
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| author | Weiwei Xue Jiacheng Du Yunteng Li Zhicheng Shao |
| author_facet | Weiwei Xue Jiacheng Du Yunteng Li Zhicheng Shao |
| author_sort | Weiwei Xue |
| collection | DOAJ |
| description | Summary: Cryopreservation of neural organoids and human living brain tissue faces huge challenges. Here, we present a cryopreservation protocol for the reliable storage of diverse neural organoids and human living brain tissue using methylcellulose, ethylene glycol, DMSO, and Y27632, termed MEDY. We describe steps for preparing cryopreservation solution, preparing cortical organoids, and surgical removal of human brain tissue. We then detail procedures for cryopreservation, resuscitation, and detection assays. This protocol can serve as a model for studying human neurological diseases.For complete details on the use and execution of this protocol, please refer to Xue et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-edc8037b5cc54b13b00fdb847d2a5b0a |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-edc8037b5cc54b13b00fdb847d2a5b0a2025-08-20T01:54:15ZengElsevierSTAR Protocols2666-16672024-12-015410346610.1016/j.xpro.2024.103466Protocol for cryopreserving neural organoids and human living brain tissue using MEDYWeiwei Xue0Jiacheng Du1Yunteng Li2Zhicheng Shao3Jinshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institute of Pediatrics, National Children’s Medical Center, Children’s Hospital, Fudan University, Shanghai 200032, China; Corresponding authorJinshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institute of Pediatrics, National Children’s Medical Center, Children’s Hospital, Fudan University, Shanghai 200032, ChinaJinshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institute of Pediatrics, National Children’s Medical Center, Children’s Hospital, Fudan University, Shanghai 200032, ChinaJinshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institute of Pediatrics, National Children’s Medical Center, Children’s Hospital, Fudan University, Shanghai 200032, China; Department of Neurology, Zhongshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan University, Shanghai 200032, China; Corresponding authorSummary: Cryopreservation of neural organoids and human living brain tissue faces huge challenges. Here, we present a cryopreservation protocol for the reliable storage of diverse neural organoids and human living brain tissue using methylcellulose, ethylene glycol, DMSO, and Y27632, termed MEDY. We describe steps for preparing cryopreservation solution, preparing cortical organoids, and surgical removal of human brain tissue. We then detail procedures for cryopreservation, resuscitation, and detection assays. This protocol can serve as a model for studying human neurological diseases.For complete details on the use and execution of this protocol, please refer to Xue et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724006312neuroscienceorganoidsstem cells |
| spellingShingle | Weiwei Xue Jiacheng Du Yunteng Li Zhicheng Shao Protocol for cryopreserving neural organoids and human living brain tissue using MEDY STAR Protocols neuroscience organoids stem cells |
| title | Protocol for cryopreserving neural organoids and human living brain tissue using MEDY |
| title_full | Protocol for cryopreserving neural organoids and human living brain tissue using MEDY |
| title_fullStr | Protocol for cryopreserving neural organoids and human living brain tissue using MEDY |
| title_full_unstemmed | Protocol for cryopreserving neural organoids and human living brain tissue using MEDY |
| title_short | Protocol for cryopreserving neural organoids and human living brain tissue using MEDY |
| title_sort | protocol for cryopreserving neural organoids and human living brain tissue using medy |
| topic | neuroscience organoids stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724006312 |
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